Critical Role of COI1-Dependent Jasmonate Pathway in AAL toxin induced PCD in Tomato Revealed by Comparative Proteomics

Abstract

Alternaria alternata f.sp. Lycopersici (AAL) toxin induces programmed cell death (PCD) in susceptible tomato (Solanum lycopersicum) leaves. Jasmonate (JA) promotes AAL toxin induced PCD in a COI1 (coronatine insensitive 1, JA receptor)-dependent manner by enhancement of reactive oxygen species (ROS) production. To further elucidate the underlying mechanisms of this process, we performed a comparative proteomic analysis using tomato jasmonic acid insensitive1 ( jai1), the receptor mutant of JA, and its wild type (WT) after AAL toxin treatment with or without JA treatment. A total of 10367 proteins were identified in tomato leaves using isobaric tags for relative and absolute quantitation (iTRAQ) quantitative proteomics approach. 2670 proteins were determined to be differentially expressed in response to AAL toxin and JA. Comparison between AAL toxin treated jai1 and its WT revealed the COI1-dependent JA pathway regulated proteins, including pathways related to redox response, ceramide synthesis, JA, ethylene (ET), salicylic acid (SA) and abscisic acid (ABA) signaling. Autophagy, PCD and DNA damage related proteins were also identified. Our data suggest that COI1-dependent JA pathway enhances AAL toxin induced PCD through regulating the redox status of the leaves, other phytohormone pathways and/or important PCD components.

In‐depth characterization of the tomato fruit pericarp proteome

“…To date, the most recent high-throughput proteomics analyses were reported by Martin et al [5] and Kilambi et al [6], who identified 3625 and 5404 proteins, respectively. Zhang et al [7] recently reported the identification of more than 10 000 proteins from tomato leaves with a global FDR of 5%. In this work, we present an in-depth characterization of the tomato fruit proteome through an extensive fractionation of tomato pericarp protein extracts.…”

“…As our dataset represents one of the largest described for S. lycopersicum L. fruit, to evaluate the stringency of our analysis workflow we compared the proteins identified in our dataset to the data from Zhang et al who recently reported the identification of more than 10 000 proteins from tomato leaves [7]. However, to get an accurate comparison, we reanalyze Zhang et al data with our search parameters.…”

“…Zhang et al. recently reported the identification of more than 10 000 proteins from tomato leaves with a global FDR of 5%. In this work, we present an in‐depth characterization of the tomato fruit proteome through an extensive fractionation of tomato pericarp protein extracts.…”

Identifikasi Ketahanan Plasma Nutfah Karet Irrdb 1981 Terpilih Terhadap Penyakit Gugur Daun Corynespora Berdasarkan Aktivitas Toksin Cassicolin

“…Detoksifikasi toksin yang masuk ke jaringan tanaman terjadi melalui reduksi kelompok karbonil penting pada toksin oleh enzim ANDPH-dependent reductase yang diekspresikan oleh tanaman yang tahan sehingga toksin jadi kehilangan kemampuan aktifitasnya. Mekanisme detoksifikasi ini mirip dengan ketahanan berbagai tanaman seperti jagung, padi, sorgum dan tebu terhadap toksin Helmithosporium Carbonum (HC) yang dihasilkan oleh Cochliobolus carbonum (Zainudin et al, 2015), tanaman tembakau terhadap toksin Alternaria Alternate (AAL) yang dihasilkan oleh Alternaria alternata (Tsuge et al, 2013;Zhang et al, 2016) dan tanaman oat terhadap toksin Victorin yang dihasilkan oleh Chocliobolus victoriae (Sweat & Wolpert, 2007;Sweat et al, 2008).…”

A COI1 gene in wheat contributes to the early defence response against wheat powdery mildew

“…The hormone jasmonate (JA) is involved with multiple biological functions, such as biotic and abiotic stress, seed germination, pollen development and cell senescence (Chini, Gimenez‐Ibanez, Goossens, & Solano, ; Wang, Liu, Niu, Timko, & Zhang, ; Zhang et al., ). Jasmonyl‐isoleucine (JA‐Ile) receptor COI1 (CORONATINE INSENSITIVE 1), a compound of the Skp‐Cullin‐F‐box (SCF) E3 ubiquitin ligase complex SCF COI1 , is a key regulator of the JA signalling pathway (Chini et al., ; Chung & Howe, ; Thines et al., ; Zhang, Bokowiec, Rushton, Han, & Timko, ).…”