“…Halevy and Guggenheim (1953) autoclaved wheat gluten-glucose mixtures and found that the in vitro digestibility of the essential amino acids was reduced by the autoclaving. In a study on the chemistry of the spontaneous heating of stored alfalfa pellets, Ruliffson et al (1956) concluded that the heating was initiated and sustained by sugar-protein interactions of the Maillard type, resulting in reduction of the free amino acid content. Beauchene and Mitchell (1957) found no difference in the total nitrogen content of alfalfa meal dehydrated at 50°C from that which was dehydrated commercially.…”