Cryobiotechnology of tropical seeds – scale, scope and hope

Seed storage of Brazilian cacti species in different threat categories

“…The acceptable moisture limit for the cryopreservation technique is specific for each species and requires individual evaluation (Salomão ; Pritchard et al . ). Nevertheless, for most cacti species the ideal storage conditions remain unknown (Rojas‐Aréchiga & Vázquez‐Yanes ; Veiga‐Barbosa et al .…”

“…Reduction of the germinability after cryopreservation can be attributed to the contamination by fungi during the germination evaluation process or to a high initial moisture of the seeds (Salomão 2002;Marchi et al 2013), which facilities the formation of intracellular ice crystals that cause ruptures in the endomembrane system and compromise cellular compartmentalization (Goldfarb et al 2010;Wesley-Smith et al 2015). The acceptable moisture limit for the cryopreservation technique is specific for each species and requires individual evaluation (Salomão 2002;Pritchard et al 2017). Nevertheless, for most cacti species the ideal storage conditions remain unknown (Rojas-Aréchiga & Vázquez-Yanes 2000; Veiga- Barbosa et al 2010;Meiado 2014;Meiado et al 2010;Nascimento et al 2015).…”

“…; Engelmann ; Pritchard et al . ). This technique is considered the most promising way to preserve the cells, tissue and vegetal organs for a long time (Carvalho & Vidal ; Walters et al .…”

“…; Pritchard et al . ). Cryopreservation can stop all cell metabolism without the danger of genetic variation in the preserved material, and it can be regenerated at any time (Walters et al .…”

“…Cryopreservation is one example of the techniques used in the formation of seed banks, and is defined as conservation of biological material in liquid nitrogen at a temperature of −196 C (Engelmann 1991;González-Benito et al 2004;Walters et al 2004;Engelmann 2011;Pritchard et al 2017). This technique is considered the most promising way to preserve the cells, tissue and vegetal organs for a long time (Carvalho & Vidal 2003;Walters et al 2004;Pritchard et al 2017). Cryopreservation can stop all cell metabolism without the danger of genetic variation in the preserved material, and it can be regenerated at any time (Walters et al 2004;Cruz-Cruz et al 2013).…”