Cryomacroscopy in 3D: A Device Prototype for the Study of Cryopreservation

Rabin, Yoed; Feig, Justin S.G.; Williams, Alexander C.; Lin, Christopher C.; Thaokar, Chandrajit

doi:10.1115/sbc2012-80527

Cited by 3 publications

(3 citation statements)

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“…Consistent with recently published studies to explain cryomacroscopy observations [28], the container material properties are assumed similar to polystyrene. Despite all of the work that has been done, some material property data for VS55 is still unavailable.…”

Section: Modelingsupporting

confidence: 53%

On the effects of thermal history on the development and relaxation of thermo-mechanical stress in cryopreservation

2014

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This study investigates the effects of the thermal protocol on the development and relaxation of thermo-mechanical stress in cryopreservation by means of glass formation, also known as vitrification. The cryopreserved medium is modeled as a homogeneous viscoelastic domain, constrained within either a stiff cylindrical container or a highly compliant bag. Annealing effects during the cooling phase of the cryopreservation protocol are analyzed. Results demonstrate that an intermediate temperature-hold period can significantly reduce the maximum tensile stress, thereby decreasing the potential for structural damage. It is also demonstrated that annealing at temperatures close to glass transition significantly weakens the dependency of thermo-mechanical stress on the cooling rate. Furthermore, a slower initial rewarming rate after cryogenic storage may drastically reduce the maximum tensile stress in the material, which supports previous experimental observations on the likelihood of fracture at this stage. This study discusses the dependency of the various stress components on the storage temperature. Finally, it is demonstrated that the stiffness of the container wall can affect the location of maximum stress, with implications on the development of cryopreservation protocols.

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Section: Modelingsupporting

confidence: 53%

On the effects of thermal history on the development and relaxation of thermo-mechanical stress in cryopreservation

2014

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“…Fracture formation is often an indication of structural failure of larger specimens such as heart valves [1], and blood vessels [2,13]. A new device has been developed recently—the cryomacroscope—to investigate fracturing events during the processes of cryopreservation [4,15,20]. Unfortunately, the correlation of structural damage with the development of thermo-mechanical stress in cryopreserved specimens represents a largely unexplored area in cryopreservation research.…”

Section: Introductionmentioning

confidence: 99%

Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators

et al. 2014

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This study provides thermal expansion data for blood vessels permeated with the cryoprotective cocktail DP6, when combined with selected synthetic ice modulators (SIMs): 12% polyethylene glycol 400, 6% 1,3-cyclohexanediol, and 6% 2,3-butanediol. The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. The current study is part of an ongoing effort to characterize thermo-mechanical effects on structural integrity of cryopreserved materials, where thermal expansion is the driving mechanism to thermo-mechanical stress. This study focuses on the lower part of the cryogenic temperature range, where the cryoprotective agent (CPA) behaves as a solid for all practical applications. By combining results obtained in the current study with literature data on the thermal expansion in the upper part of the cryogenic temperature range, unified thermal expansion curves are presented.

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“…The system configuration has been presented elsewhere [4], and is discussed here in brief for the completeness of the report. With reference to Fig.…”

Section: Methodsmentioning

confidence: 99%

Developing the cryomacroscope for cryopreservation applications

Feig

Williams

Lin

et al. 2012

2012 38th Annual Northeast Bioengineering Conference (NEBEC)

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This study concerns the development of a new device prototype to address the unmet need for visualization of physical effects associated with large-scale cryopreservation--the preservation of tissues at very low temperatures. An early prototype of this device has been presented in 2005, and was termed the cryomacroscope. The goal in the current study is to develop an advanced-stage prototype of the cryomacroscope, with the following design objectives: (1) to visualize and record the process of cryopreservation in situ, (2) to design the device as an add-on unit for commercially available controlled-rate freezers, (3) to enable scanning of large samples, (4) to enable identification of physical effects such as ice formation and fracturing, and (5) to integrate data into a single movie, including time, temperature, and camera position. Results of this study demonstrate glass formation, crystallization, and fracture formation during a typical cryopreservation protocol.

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Cryomacroscopy in 3D: A Device Prototype for the Study of Cryopreservation

Cited by 3 publications

References 0 publications

On the effects of thermal history on the development and relaxation of thermo-mechanical stress in cryopreservation

On the effects of thermal history on the development and relaxation of thermo-mechanical stress in cryopreservation

Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators

Developing the cryomacroscope for cryopreservation applications

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