Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos

Linhart, Otomar; Rodina, Marek; Cosson, Jacky

doi:10.1006/cryo.2000.2284

Cited by 187 publications

(90 citation statements)

References 18 publications

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“…This may be due to differences in extender, cryoprotectant, equilibration, egg quality, or protocol. In the present study, the interaction between the percentage of motile post-thaw sperm and fertilizing capacity was highly positive, similar to results in common carp (Linhart et al, 2000), African catfish (Rurangwa et al, 2001) and grass carp (Bozkurt et al, 2011b).…”

Section: Ornamental Koi Carp (Cyprinus Carpio)supporting

confidence: 90%

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Bozkurt¹,

Yavaş²,

Karaca³

2012

Current Frontiers in Cryopreservation

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Section: Ornamental Koi Carp (Cyprinus Carpio)supporting

confidence: 90%

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Bozkurt¹,

Yavaş²,

Karaca³

2012

Current Frontiers in Cryopreservation

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“…Magyary et al (26) concluded a strong, positive correlation of frozen-thawed carp spermatozoa motility and fertility at 1-1.5x10 5 :1 spermatozoa/egg ratio. In addition, Linhart et al (25) also observed good correlation with fresh sperm between carp spermatozoa motility and fertilization at 2x10 5 :1 sperm/egg ratio. The recommended sperm to egg ratio for trout culture is based on the use of stripped milt (33).…”

Section: Discussionmentioning

confidence: 87%

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Hatipoğlu¹,

Akçay²

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentration and pH), the pooled samples were diluted at a 1:2 ratio with two extenders (0.3 M glucose or Ringer solution). The diluted semen was stored for 48 hours at 4°C. Following the cooled storage, motility of the spermatozoa was evaluated after 24 and 48 hours regarding the post-cooled period. Dry fertilization technique was used for the fertilization trials. Eggs were pooled from 30 females by the hand stripping method. Fertilization took place in dry plastic dishes and 600 eggs were used in each fertilization trial. The sperm-egg ratio was approximately 0.25x10 6 sperm/egg. After swelling, eggs were rinsed with hatchery water (7 o C) and batches were placed into vertical incubation trays. The experimental success was assessed from sperm motility and the percent of eyed-egg 25 days after fertilization. Average fresh semen motility was 81.4 %. According to the results of the experiment, the highest motility (67 % after 24 h, 53 % after 48 h) and movement duration (60 s after 24 h, 42 s after 48 h) were determined by using glucose extender after 24 and 48 hours storage. Fertility rate of fresh semen was 84.4 %. The highest eyed-egg rate (80.4 %) was obtained from semen stored with glucose based extender after 24 h storage. After 48 hour storage fertilization yield for the semen diluted with 0.3 M Glucose decreased to 61.9 % and to 43.8 % for the one diluted with Ringer solution. Our results indicate that glucose based extender is a better preservative than Ringer solution for the short term preservation of Abant trout semen.Key words: Abant trout, semen, short-term preservation, fertilityKısa süreli saklanmış Abant alabalığı spermatozoonlarının fertilizasyon yeteneği Özet: Bu araştırma ile, ülkemize ait endemik bir balık türü olan Abant alabalığının spermatolojik parametrelerinin değerlendirilmesi ve farklı sulandırıcılarda kısa süreli saklanan spermatozoonların fertilizasyon yeteneğinin ortaya konulması amaçlanmıştır. Bu araştırmada, 30 ergin erkek balıktan anestezi yapılmaksızın masaj yoluyla sperma alınmıştır. Birincil spermatolojik parametreler (miktar, motilite, haraket süresi, yoğunluk ve pH) belirlendikten sonra birleştirilen örnekler iki farklı sulandırıcı ile (0,3 M glukoz veya Ringer solusyonu) 1:2 (sperma:sulandırıcı) oranında sulandırılmıştır. Sulandırılan sperma 4°C'de 48 saat saklanmıştır. Saklama işlemi esnasında 24. ve 48. saatlerde motilite değerlendirmesi yapılmıştır. Fertilizasyon denemeleri için kuru fertilizasyon tekniği kullanılmıştır. Yumurtalar 30 ergin dişiden masaj yoluyla alınmıştır. Fertilizasyon kuru plastic kaplarda gerçekleştirilmiş ve her bir fertilizasyon denemesi için 600 yumurta ku...

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“…The subsequent two trials expanded the concentration range further from 5 to 20% for DMSO and glycerol with sperm from two males used for each cryoprotectant. In the third trial, the final concentrations of DMSO were 5,8,10,12,14,17, and 20%, and motility was estimated at 10 min, 4, 16, 28, 40, and 64 h after thawing. In the fourth trial, the final concentrations of glycerol were 5, 8, 11, 14, 17, and 20%, and motility was estimated at 10 min, 4,16,40, 64, 88, 112, 136, 160, 184, and 208 h after thawing.…”

mentioning

confidence: 99%

Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization

et al. 2004

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Sperm cryopreservation for fishes with internal fertilization is essentially unexplored although many species of these fishes are valuable biomedical research models. To explore methods for sperm cryopreservation within the live-bearing genus Xiphophorus, this study used X. helleri to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio. Sperm motility and survival duration after thawing showed significant differences among different cryoprotectants with the highest motility at 10 min after thawing obtained with 14% glycerol. With subsequent use of 14% glycerol as the cryoprotectant, the highest motility after thawing was observed with Hanks' balanced salt solution (HBSS) at 300 mOsmol/kg. Samples cooled from 5 to −80 °C at 20 °C/min yielded the highest post-thaw motility although no significant difference was found in the first 4 h after thawing for cooling rates across the range of 20-35 °C/min. Evaluation of equilibration time revealed no significant difference between 20 min and 2 h, but the highest motility at 10 min after thawing was found with a 20-min equilibration. Dilution ratios of sperm-to-extender at 1:20, 1:60, and 1:120 showed no significant differences in motility and survival duration after thawing, but the dilution of sperm solutions with HBSS (320 mOsmol/kg) immediately after thawing reduced the decline of sperm motility, and significantly prolonged the survival duration. Based on these findings, the highest average sperm motility (77%) at 10 min after thawing was obtained when sperm were suspended in HBSS at 300 mOsmol/kg with 14% glycerol as cryoprotectant, diluted at a ratio of sperm to HBSS-glycerol of 1:20, equilibrated for 10 min, cooled at 20 °C/min from 5 to −80 °C before being plunged in liquid nitrogen, and thawed in a 40 °C water bath for 7 s. If diluted immediately after thawing, sperm frozen by the protocol above retained continuous motility after thawing for more than 8 days when stored at 4 °C. The first studies of fish sperm cryopreservation were published 50 years ago [5], and since then more than 200 fish species have been studied [26,39]. This research effort has yielded techniques that are being applied with varying levels of success for fishes around the world. However, most work has focused on large-bodied culture and sport fishes, such as salmon and trout of the family salmonidae [29], carps of the family cyprinidae [11], and catfishes of the families claridae, ictaluridae, pangasiidae, and siluridae [35]. Small teleost fishes are studied much less except for the research models zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) [1]. Published studies of sperm cryopreservation of small livebearing fish are completely lacking except for our initial research on swordtail sperm [12]. Live-bearing fishes as a group are distinct from other fishes for many reasons, most notably for internal fertilization in which males transfer sperm in packages (spermatophores) to females which store the sp...

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Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos

Cited by 187 publications

References 18 publications

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Cryopreservation of Brown Trout (Salmo trutta macrostigma) and Ornamental Koi Carp (Cyprinus carpio) Sperm

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization

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