Cryopreservation of sperm in marine fish

Abstract

Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen–thawed semen was evaluated using previously standardized biotests, such as a two‐step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results. Cooling rates range from 8 °C to 99 °C min−1; the thawing rate is generally high. Compared with freshwater species, a high percentage of spermatozoa survives cryopreservation. Therefore, and because of the simplicity of the techniques, the cryopreservation of marine fish sperm is suited for application in aquaculture.

Rapid assessment of genotype-by-environment interactions and heritability for growth rate in aquaculture species using in vitro fertilisation and DNA tagging

“…Collection of sperm has now been demonstrated in up to 30 marine fish species (Suquet et al, 2000) with egg collection (Sahoo et al, 2008) and artificial fertilisation successful in producing multiple sires (Palmer et al, 1993;Quinton et al, 2007;DupontNivet, 2008). …”

Effect of Different Cryoprotectants and Sperm Densities of Orange Mud Crab, Scylla olivacea (Herbst, 1796) for Long-Term Storage of Spermatozoa

“…Off season spawning can be induced in cultured species, but the techniques are cost intensive. With the availability of cryopreserved mud crab sperm throughout the year, manipulation of the spawning season could be restricted to only female crabs (Suquet et al 2000). Despite the increasing interest in mud crab farming, very little information exists on mud mud crab breeding in detailed (Noorbaiduri et al 2014;Quinitio & Parado-Estapa 2008;Shelley & Lovatelli 2011).…”

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

“…(Suquet et al 2000). For this reason, effective sperm cryopreservation procedure has been adviced to the aquaculture sector to improve their culture techniques (Liu et al 2010).…”