Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability

Basavaraja, N.; Hegde, S.N.

doi:10.1016/j.cryobiol.2004.05.007

Cited by 32 publications

(25 citation statements)

References 7 publications

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“…Theoretically, optimally cryopreserved sperm can be stored indefinitely and viably for many years in liquid nitrogen (À196°C) as metabolism is drastically reduced at subzero temperatures (Ashwood-Smith 1980). However, failures in terms of sperm motility and viability among studies may be partly attributed to cryoinjury and/or oxidative damage to spermatozoa during cryopreservation, the latter, by generation of reactive oxygen species (Basavaraja & Hegde 2004). Oxidative damage during sperm cryopreservation has been known to induce lipid peroxidation, which affected spermatozoa structure and function and DNA damage (Watson 2000;Chen et al 2010;Li, Hulak, Koubek, Sulc, Dzyuba, Boryshpolets, Rodina, Gela, Manaskova-Postlerova, Peknicova & Linhart 2010).…”

Section: Effect Of Storage Period Of Cryopreserved Sperm On Post-thawmentioning

confidence: 99%

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

et al. 2014

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Effectiveness and efficiency of frozen sperm on fertilization and hatching success of eggs from silver barb was examined in relation to cryoprotectants, freezing rate and storage period. Sperm was diluted in calcium-free Hank's balanced salt solution, equilibrated with dimethylsulphoxide (DMSO), propylene glycol, sucrose or methanol at 5%, 10%, 15% or 20% final concentrations, and frozen in 250-lL straws using a one-step freezing procedure (1, 5 and 8°C min À1 from 25 to À40°C). Highest post-thaw sperm motility was found from a treatment using 10% DMSO and 5°C min À1 (82.2 AE 2.1%), similar to that of 10% DMSO and 8°C min À1 (87.8 AE 3.2%). Post-thaw motility of sperm frozen at 5 or 8°C min À1 was significantly higher than 1°C min À1 . Relative sperm motility declined significantly after 10 months of cryostorage while viability did not change during a 12-month cryostorage. Average fertilization rates of sperm after 1 and 4 months of storage were 64.5 AE 4.6% and 61.3 AE 3.4%, respectively, similar to those of fresh sperm (69.6-72.3%). Hatching rates of cryopreserved sperm (45.4-51.2%) were similar to those of fresh sperm (51.8-57.8%). This study developed suitable methods for cryopreservation of silver barb sperm that can be used to facilitate hatchery operation.

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Section: Effect Of Storage Period Of Cryopreserved Sperm On Post-thawmentioning

confidence: 99%

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

et al. 2014

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“…Conservation-oriented methods have been developed for various taxa, including acipenseriform fish (Ciereszko et al, 2006; Horvath et al, 2005), salmonids (Martínez-Páramo et al, 2009b; Nynca et al, 2015c; Sarvi et al, 2006), cyprinids (Basavaraja and Hegde, 2004; Tiersch et al, 2004) and other fish species (Asturiano et al, 2003; Maria et al, 2006; Orfão et al, 2011). In addition, studies have been carried out to assess the effect of cryopreservation on the genetic diversity of some species (Martínez-Páramo et al, 2009b; Van Der Walt et al, 1993).…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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“…Thus, one of the most critical steps in successful cryopreservation of fish semen is the choice of cryoprotectant in the extender during the process. In this concept, methanol has been used as permeable cryoprotectant widely for cryopreservation of sperm from different species such as African catfish (Clarias gariepinus) (Steyn and Van Vuren 1987), tilapia (Sarotherodon mossambicus) (Rana and McAndrew 1989), bagrid catfish (Mystus nemurus) (Muchlisin et al 2004), silver carp (Hypophthalmichtys molitrix) (Alvarez et al 2003), mahseer (Tor khudree) (Basavaraja and Hegde 2004), tench (Tinca tinca) (Rodina et al 2007), and salmonid fish (Lahnsteiner et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Bozkurt

Yavaş

2017

Journal of Limnology and Freshwater Fisheries Research

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Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straw volumes and thawing rates on the postthaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4 °C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120 ºC) and finally stored in liquid nitrogen (-196 ºC) tank. Thawing of cryopreserved semen process was performed at 30 ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using a ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30 ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

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Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability

Cited by 32 publications

References 7 publications

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

Cryobanking of aquatic species

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

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