Cryopreserved human blastocysts after vitrification result in excellent implantation and clinical pregnancy rates

Hong, Seung Wook; Sepilian, Vicken; Chung, Hyung Min; Kim, Thomas Jin

doi:10.1016/j.fertnstert.2009.06.008

Cited by 26 publications

(17 citation statements)

References 17 publications

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“…Moreover, some authors declare that cohort cryopreservation might convey an a priori greater chance of success [24]. At the same time, the development of vitrification and improved long-term in vitro culture systems lead to the better survival rate of frozen-thawed embryos, limiting cellular damage and enabling treated embryos to maintain the reproductive potential, comparable to fresh ones [43,50,51].…”

Section: Discussionmentioning

confidence: 99%

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

Greco

Litwicka

Arrivi

et al. 2016

J Assist Reprod Genet

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Purpose The aim of the study was to evaluate two methods of endometrial preparation for frozen-thawed single euploid blastocyst transfer: modified natural and artificial cycle with GnRH-agonist pituitary suppression. Methods In this prospective, controlled randomized trial, a total of 236 patients undergoing infertility treatment were randomized in 1:1 ratio; 118 received a frozen-thawed single euploid blastocyst transfer in a modified natural cycle and 118 in an artificial cycle with GnRH-agonist pituitary suppression. In the artificial protocol, GnRH-agonist combined with estradiol valerate was administered. In the natural protocol, only final oocyte maturation was induced using human chorionic gonadotropin administration. The primary end-points were the clinical pregnancy and implantation rates; the secondary end-points were the cost-benefit in terms of drug cost and the number of visits and the woman psychological distress caused by the treatment. Results No significant differences were found in clinical pregnancy, implantation, and miscarriage rates between protocols. The number of clinical and ultrasound controls and the number of laboratory dosages and venous samplings were similar in both study groups. No significant differences were found between the groups in the anxiety and depression values before the start of treatment, on the days of progesterone administration, the blastocyst transfer, and pregnancy test. Conclusions The findings of this study evidence that in case of frozen-thawed single euploid blastocyst transfer, both protocols are equally effective in terms of clinical outcomes, costbenefit, and patient compliance. The choice of endometrial preparation protocol should be based on women menstrual and ovulatory characteristics or otherwise on patient need for cycle planning. Trial registration www.clinicaltrials.gov with number NCT02378584

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Section: Discussionmentioning

confidence: 99%

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

Greco

Litwicka

Arrivi

et al. 2016

J Assist Reprod Genet

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“…Hong et al reported improved clinical results between two cohorts in the same laboratory. They modified the protocol due to the finding that the permeation of the cryoprotectant into the embryo was faster at a higher temperature [6]. Minimum vitrification solution is used in Cryotop method.…”

Section: Discussionmentioning

confidence: 99%

“…The vitrified blastocysts retained good developmental competency after thawing. High pregnancy and implantation rates can be expected once the blastocysts go through vitrification and thawing because they have the potential to develop as fresh blastocysts [6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the clinical outcomes between fresh blastocyst and vitrified-thawed blastocyst transfer

Lee

Lin

et al. 2012

J Assist Reprod Genet

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“…S ince initial reports on pregnancies were achieved from frozen and thawed human embryos (1,2), effective, clinically applicable methods have been developed to cryopreserve human gametes (3)(4)(5) and embryos at different developmental stages: pronuclear (6,7), cleavage (8), morula (9), and blastocyst (10)(11)(12). Presently, cryopreserved-thawed or warmed embryos constitute about 20% of all transfers performed in assisted reproduction clinics worldwide (13).…”

mentioning

confidence: 99%

Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

Valbuena

Póo

Aguilar-Gallardo

et al. 2012

Fertility and Sterility

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Cryopreserved human blastocysts after vitrification result in excellent implantation and clinical pregnancy rates

Cited by 26 publications

References 17 publications

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist

Comparison of the clinical outcomes between fresh blastocyst and vitrified-thawed blastocyst transfer

Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

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