Crystal structure of human anterior gradient protein 3

Nguyen, Van Dat; Biterova, Ekaterina; Salin, Mikko; Wierenga, Rik K.; Ruddock, Lloyd W.

doi:10.1107/s2053230x18009093

Cited by 6 publications

(3 citation statements)

References 35 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compromised mucociliary clearance contributes to changes in relative proportions and viscosity of host mucins, which, in turn, contribute to airway obstruction [ 68 ]. AGR3 was shown to interact with mucins [ 69 ] and this may explain its binding to Tc-MUC-3, which is a mucin-like glycoprotein [ 13 ]. It remains to be confirmed if, and to what extent, this interaction may contribute to the pathology of toxocariasis.…”

Section: Discussionmentioning

confidence: 99%

Identification of Toxocara canis Antigen-Interacting Partners by Yeast Two-Hybrid Assay and a Putative Mechanism of These Host–Parasite Interactions

2021

View full text Add to dashboard Cite

Toxocara canis is a zoonotic roundworm that infects humans and dogs all over the world. Upon infection, larvae migrate to various tissues leading to different clinical syndromes. The host–parasite interactions underlying the process of infection remain poorly understood. Here, we describe the application of a yeast two-hybrid assay to screen a human cDNA library and analyse the interactome of T. canis larval molecules. Our data identifies 16 human proteins that putatively interact with the parasite. These molecules were associated with major biological processes, such as protein processing, transport, cellular component organisation, immune response and cell signalling. Some of these identified interactions are associated with the development of a Th2 response, neutrophil activity and signalling in immune cells. Other interactions may be linked to neurodegenerative processes observed during neurotoxocariasis, and some are associated with lung pathology found in infected hosts. Our results should open new areas of research and provide further data to enable a better understanding of this complex and underestimated disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Toxocara canis Antigen-Interacting Partners by Yeast Two-Hybrid Assay and a Putative Mechanism of These Host–Parasite Interactions

2021

View full text Add to dashboard Cite

show abstract

“…It is expressed in airway epithelium where it is required for muco-ciliary clearance and for regulation of ciliary beat frequency (32). It is also expressed in prostate, as well as stomach and liver tissues of the digestive tract (33).…”

Section: Genes Structures and Regulatorsmentioning

confidence: 99%

Anterior gradient proteins in gastrointestinal cancers: from cell biology to pathophysiology

et al. 2022

View full text Add to dashboard Cite

“…AGR2 and AGR3 expression are coupled in EsR positive breast cancer and the combination of AGR2 and AGR3 as biomarkers resulted in increased sensitivity and specificity thus suggesting that they can be used as a prognostic factor [ 113 ]. The crystal structure of AGR3 (PDB code:3PH9) has revealed that it can form an asymmetric unit consisting of two AGR3 molecules, however, there was no evidence that AGR3 can form a spontaneous dimer in solution [ 114 ].…”

Section: Functions and Mechanisms Of Pdi Members In Cancermentioning

confidence: 99%

Functions and mechanisms of protein disulfide isomerase family in cancer emergence

et al. 2022

View full text Add to dashboard Cite

The endoplasmic reticulum (ER) is a multi-layered organelle that is essential for the synthesis, folding, and structural maturation of almost one-third of the cellular proteome. It houses several resident proteins for these functions including the 21 members of the protein disulfide isomerase (PDI) family. The signature of proteins belonging to this family is the presence of the thioredoxin domain which mediates the formation, and rearrangement of disulfide bonds of substrate proteins in the ER. This process is crucial not only for the proper folding of ER substrates but also for maintaining a balanced ER proteostasis. The inclusion of new PDI members with a wide variety of structural determinants, size and enzymatic activity has brought additional epitomes of how PDI functions. Notably, some of them do not carry the thioredoxin domain and others have roles outside the ER. This also reflects that PDIs may have specialized functions and their functions are not limited within the ER. Large-scale expression datasets of human clinical samples have identified that the expression of PDI members is elevated in pathophysiological states like cancer. Subsequent functional interrogations using structural, molecular, cellular, and animal models suggest that some PDI members support the survival, progression, and metastasis of several cancer types. Herein, we review recent research advances on PDIs, vis-à-vis their expression, functions, and molecular mechanisms in supporting cancer growth with special emphasis on the anterior gradient (AGR) subfamily. Last, we posit the relevance and therapeutic strategies in targeting the PDIs in cancer.

show abstract

Crystal structure of human anterior gradient protein 3

Cited by 6 publications

References 35 publications

Identification of Toxocara canis Antigen-Interacting Partners by Yeast Two-Hybrid Assay and a Putative Mechanism of These Host–Parasite Interactions

Identification of Toxocara canis Antigen-Interacting Partners by Yeast Two-Hybrid Assay and a Putative Mechanism of These Host–Parasite Interactions

Anterior gradient proteins in gastrointestinal cancers: from cell biology to pathophysiology

Functions and mechanisms of protein disulfide isomerase family in cancer emergence

Contact Info

Product

Resources

About