Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7

Basak, A.K.; Gouet, Patrice; Grimes, Jonathan M.; Roy, P; Stuart, D.I.

doi:10.1128/jvi.70.6.3797-3806.1996

Cited by 55 publications

(34 citation statements)

References 50 publications

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“…The proliferation of side-to-side P2 interactions favours lateral crystal growth, reflecting the plate-like crystal morphology. Interestingly, this packing appears to recapitulate some of the molecular interactions seen in the virus capsid, an effect previously reported for African horse sickness virus (AHSV; Basak et al, 1996). However, whilst the arrangement of trimeric coat proteins in viruses of the PRD1adenovirus lineage has local p3 symmetry (Abrescia et al, 2004), adjacent rows of trimers in Fig.…”

Section: X-ray Data Analysissupporting

confidence: 63%

Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

et al. 2005

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PM2 (Corticoviridae) is a dsDNA bacteriophage which contains a lipid membrane beneath its icosahedral capsid. In this respect it resembles bacteriophage PRD1 (Tectiviridae), although it is not known whether the similarity extends to the detailed molecular architecture of the virus, for instance the fold of the major coat protein P2. Structural analysis of PM2 has been initiated and virus-derived P2 has been crystallized by sitting-nanodrop vapour diffusion. Crystals of P2 have been obtained in space group P2 1 2 1 2, with two trimers in the asymmetric unit and unit-cell parameters a = 171.1, b = 78.7, c = 130.1 Å . The crystals diffract to 4 Å resolution at the ESRF BM14 beamline (Grenoble, France) and the orientation of the non-crystallographic threefold axes, the spatial relationship between the two trimers and the packing of the trimers within the unit cell have been determined. The trimers form tightly packed layers consistent with the crystal morphology, possibly recapitulating aspects of the arrangement of subunits in the virus.

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Section: X-ray Data Analysissupporting

confidence: 63%

Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

et al. 2005

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“…Earlier studies associated a conserved Arg-Gly-Asp ( 167 RGD 171 ) motif on the T13 protein of BTV with the attachment to Culicoides cells [90,91]. This conserved motif was also found by later studies in some species closely related to BTV [43,48,70], but we did not observed it in LEAV.…”

Section: Discussionsupporting

confidence: 68%

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Tomazatos

Marschang

Maranda

et al. 2020

Viruses

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The discovery and characterization of novel arthropod-borne viruses provide valuable information on their genetic diversity, ecology, evolution and potential to threaten animal or public health. Arbovirus surveillance is not conducted regularly in Romania, being particularly very scarce in the remote and diverse areas like the Danube Delta. Here we describe the detection and genetic characterization of a novel orbivirus (Reoviridae: Orbivirus) designated as Letea virus, which was found in grass snakes (Natrix natrix) during a metagenomic and metatranscriptomic survey conducted between 2014 and 2017. This virus is the first orbivirus discovered in reptiles. Phylogenetic analyses placed Letea virus as a highly divergent species in the Culicoides-/sand fly-borne orbivirus clade. Gene reassortment and intragenic recombination were detected in the majority of the nine Letea virus strains obtained, implying that these mechanisms play important roles in the evolution and diversification of the virus. However, the screening of arthropods, including Culicoides biting midges collected within the same surveillance program, tested negative for Letea virus infection and could not confirm the arthropod vector of the virus. The study provided complete genome sequences for nine Letea virus strains and new information about orbivirus diversity, host range, ecology and evolution. The phylogenetic associations warrant further screening of arthropods, as well as sustained surveillance efforts for elucidation of Letea virus natural cycle and possible implications for animal and human health.

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“…Conserved nt sequences of 6 nt were observed at the ends of the 5′ and 3′ UTRs (5′-GTAAAA A / T and C / A ACTTAC-3′), and 4 nt in the terminal sequences were reverse complementary. The 5′ and 3′ UTRs of DH13C120 comprised 3.80% of the total genome; the G + C content of DH13C120 was 42.59%, similar to Culicoides -borne Orbivirus species; and an Arg–Gly–Asp (RGD) motif [ 15 – 17 ] was observed at position 168–170 in the viral outer core protein T13 (VP7) of DH13C120.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China

Wang

et al. 2017

Virol J

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Background Culicoides-borne orbiviruses, such as bluetongue virus (BTV) and African horse sickness virus (AHSV), are important pathogens that cause animal epidemic diseases leading to significant loss of domestic animals. This study was conducted to identify Culicoides-borne arboviruses and to investigate the associated infections in local livestock in Yunnan, China.Methods Culicoides were collected overnight in Mangshi City using light traps during August 2013. A virus was isolated from the collected Culicoides and grown using baby hamster kidney (BHK-21), Vero, Madin-Darby bovine kidney (MDBK) and Aedes albopictus (C6/36) cells. Preliminary identification of the virus was performed by polyacrylamide gel (PAGE) analysis. A full-length cDNA copy of the genome was amplified and sequenced. Serological investigations were conducted in local cattle, buffalo and goat using plaque-reduction neutralization tests.ResultsWe isolated a viral strain (DH13C120) that caused cytopathogenic effects in BHK-21, Vero, MDBK and C6/36 cells. Suckling mice inoculated intracerebrally with DH13C120 showed signs of fatal neurovirulence. PAGE analysis indicated a genome consisting of 10 segments of double-stranded RNA that demonstrated a 3–3–3–1 pattern, similar to the migrating bands of Tibet orbivirus (TIBOV). Phylogenetic analysis of the viral RNA-dependent RNA polymerase (Pol), sub-core-shell (T2, and outer core (T13) proteins revealed that DH13C120 clustered with TIBOV, and the amino acid sequences of DH13C120 virus shared more than 98% identity with TIBOV XZ0906. However, outer capsid protein VP2 and outer capsid protein VP5 shared only 43.1 and 79.3% identity, respectively, indicating that the DH13C120 virus belongs to TIBOV, and it may represent different serotypes with XZ0906. A serosurvey revealed the presence of neutralizing antibodies with 90% plaque-reduction neutralization against TIBOV DH13C120 in local cattle (44%), buffalo (20%), and goat (4%). Four-fold or higher levels of TIBOV-2-neutralizing antibody titers were detected between the convalescent and acute phases of infection in local livestock.ConclusionsA new strain of TIBOV was isolated from Culicoides. This study provides the first evidence of TIBOV infection in livestock in Yunnan, China, and suggests that TIBOV could be a potential pathogen in livestock.

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Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7

Cited by 55 publications

References 50 publications

Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes (Natrix natrix)

Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China

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