Crystalline cellulose degradation by a strain of Cellulomonas and its mutant derivatives

Haggett, K. D.; Gray, P. P.; Dunn, Noel W.

doi:10.1007/bf00506182

Cited by 41 publications

(14 citation statements)

References 6 publications

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“…Cellulomonas strains degrade cellulose from various sources, including crystalline cellulose (cotton and A vicel) (Choi et ai., 1978;Haggett et al, 1979;Kim and Wimpenny, 1981;Nakamura and Kitamura, 1983). In addition, it has been demonstrated with strain CS 1-1 that hemicelluloses are hydrolyzed by Cellulomonas species, which contain ,g-glucosidase, ,g-xylosidase, ,g-mannosidase, and ,g-arabinosidase (Rickard and Laughlin, 1980;Ide et al, 1983).…”

Section: Aerobic Cellulolytic Bacteriamentioning

confidence: 94%

Ecology of Microbial Cellulose Degradation

Ljungdahl

Eriksson

1985

Advances in Microbial Ecology

274

139

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Section: Aerobic Cellulolytic Bacteriamentioning

confidence: 94%

Ecology of Microbial Cellulose Degradation

Ljungdahl

Eriksson

1985

Advances in Microbial Ecology

274

139

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“…The isolation and characterization of CSl-1 and the mutant derivative CSl-17 have been described previously (Choi et al, 1978;Haggett et al, 1979;Choudhury et al, 1980). The substrate used in these experiments was the fraction of sugar cane bagasse with a particle size less than 0.7 mm.…”

Section: Materials I! Methodsmentioning

confidence: 99%

Saccharification of sugar cane bagasse by an enzyme preparation fromCellulomonas: Resistance to product inhibition

1980

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“…The activity of carboxymethylcellulase (CMCase) and β-D-mannanase were assayed by determining the reducing sugars produced from CMC and gum locust bean (mannan) as glucose or mannose, respectively (Haggett et al 1979;Araujo and Ward 1990). One unit was defined as enzyme which liberates one p.mol glucose or mannose per minute.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

“…Saccharification activities were determined following determination of avicelase (Haggett et al 1979) using PKC and dry POME as substrates. The incubation time of the reaction was two hours and reducing sugars produced was determined with DNS method (Miller 1959).…”

Section: Saccharification Activity Towards Pkc and Dry Pomementioning

confidence: 99%

Synergistic Activity of Enzymes Produced by Eupenicillium Javanicum and Aspergillus Niger NRRL 337 on Palm Oil Factory Wastes

Purwadaria¹,

Nirwana

Ketaren³

et al. 2003

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The use of palm kernel cake (PKC) and palm oil mill effluent (POME), substances from palm oil factory wastes, for monogastric is limited by their high cellulose and mannan contents. Hydrolytic enzymes have been supplemented to increase the nutrient digestibility. The maximal digestibility was obtained in the synergistic action of all enzyme components including B-D-endoglucanase (CMCase), B-D-glucosidase, B-D-mannanase, p-D-mannosidase, and oc-Dgalactosidase. Two kinds of enzymes produced by Eupenicillium javanicum and Aspergillus niger NRRL 337 on the submerged culture containing 3% coconut meal were selected to hydrolyze PKC or dry POME. Enzyme from E. javanicum contained higher CMCase, B-D-mannanase, and a-D-galactosidase activities, while that from A. niger NRRL 337 contained more p-D-glucosidase and p-D-mannosidase activities. Saccharification (hydrolytic) activities of enzyme mixtures on PKC and POME were determined at pH 5.0, the optimal pH for p-D-mannanase from E. javanicum, and at 5.4 the optimal pH for a-D-galactosidase from E. javanicum and P-D-glucosidase from A. niger NRRL 337. The enzyme proportions of E. javanicum and A. niger NRRL 337 were 100 : 0, 80 : 20, 60 : 40, 40 : 60, and 0 : 100%. The highest Saccharification activity on both substrates was observed on the mixture of 80% A. niger NRRL 337. The pH levels did not significantly affect Saccharification activity. Fiber components in PKC were more digestable than in POME. Further analysis on the reducing sugar components using thin layer chromatography showed that more monomers were produced in the 60 or 80% of A. niger NRRL 337. The glycosidases of A. niger NRRL 337 played more important role in the Saccharification activity.

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Crystalline cellulose degradation by a strain of Cellulomonas and its mutant derivatives

Cited by 41 publications

References 6 publications

Ecology of Microbial Cellulose Degradation

Ecology of Microbial Cellulose Degradation

Saccharification of sugar cane bagasse by an enzyme preparation fromCellulomonas: Resistance to product inhibition

Synergistic Activity of Enzymes Produced by Eupenicillium Javanicum and Aspergillus Niger NRRL 337 on Palm Oil Factory Wastes

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