Crystallization, Characterization and Metabolic Regulation of Two Types of Pyruvate Kinase Isolated from Rat Tissues*

Tanaka, Takehiko; Harano, Yutaka; Sue, Fumiaki; Morimura, Hiroko

doi:10.1093/oxfordjournals.jbchem.a128639

Cited by 449 publications

(174 citation statements)

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“…2. The pyruvate kinase mobilities observed are in agreement with previous observations (Tanaka et al, 1967a;Pogson, 1968b). Jacob & Bhargava (1962) or Branster & Morton (1957) bear out the conclusions of Suzangar & Dickson (1970) in that very little, if any, cytoplasmic enzyme activity is retained.…”

Section: Enzyme Assayssupporting

confidence: 93%

“…Several workers (Tanaka et Vol. 126 al., 1965;Passeron et al, 1967;Tanaka et al, 1967a;Taylor & Bailey, 1967;Susor & Rutter, 1968) have shown the existence in liver of at least two distinct activities, one apparently similar to, although not identical with (Jimenez de Asua et al, 1971), muscle 'M-type' pyruvate kinase, the other exhibiting both a sigmoid rate response towards the substrate phosphoenolpyruvate and a sensitivity to activation by fructose 1,6-diphosphate (Tanaka et al, 1967b;Taylor & Bailey, 1967;Carminatti et al, 1968). The presence of the latter type of pyruvate kinase in tissues has been correlated with the presence of an active dicarboxylate shuttle and gluconeogenic or glyceroneogenic potential (Pogson, 1968a;Llorente et al, 1970).…”

mentioning

confidence: 99%

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Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

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Parenchymal cells contain both glucose 6-phosphatase and fructose 1,6-diphosphatase. The non-parenchymal fraction appears to contain fructose 1,6-diphosphatase, but is devoid of glucose 6-phosphatase. 8. No aldolase A was detectable in the whole liver. Aldolase B occurs in both parenchymal and non-parenchymal tissue. 9. Parenchymal cells prepared by mechanical disruption ofmouse liver with 20 % polyvinyl alcohol exhibit a similar enzyme profile to those prepared enzymically. 10. The methodology involved in the preparation of isolated liver cells is discussed. The importance of the measurement of several parameters as criteria for establishing the viability of parenchymal cells is stressed. 11. The metabolic implications of the results in the present study are discussed.

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Section: Enzyme Assayssupporting

confidence: 93%

mentioning

confidence: 99%

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

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“…Pyruvate kinase was assayed by a method based on that of Tanaka et al [2]. Liver was homogenised in 9 vol of 0.15 M KC1 containing 5 mM MgS04, 10 mM mercaptoethanol, 5 mM EDTA and 2 rng/ml bovine plasma albumin, final pH 7.3.…”

Section: Methodsmentioning

confidence: 99%

Comparison of ability of Mg and Mn to activate the key enzymes of glycolysis

Wimhurst

Manchester

1972

FEBS Letters

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“…The three main isozymes, i.e., type L, type Mr and type Ms, have been highly purified and their molecular natures have been investigated. Type L differs from type M1 or type Mz in immunological properties [ 1,2] and amino acid composition ]3,4] , and thus considered to be a product of separate gene. On the other hand, type Mr and type Mz have very similar in ~unolo~c~ properties [S] and amino acid compositions [3,4], and thus it still uncertain whether they are different gene products f6 ,7] or not [8].…”

Section: In~du~tionmentioning

confidence: 99%