CSN1S2 protein of goat milk inhibits the decrease of viability and increases the proliferation of MC3T3E1 pre-osteoblast cell in methyl glyoxal exposure

Chotimah, Choirunil; Ciptadi, Gatot; Setiawan, Bambang; Fatchiyah, Fatchiyah

doi:10.1016/s2222-1808(14)60657-5

Cited by 13 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a growing interest in unraveling the intracellular signaling pathways by RAGE controls these disease-related processes ( 19 ). In vitro study showed high dose of CSN1S2 protein of goat milk (0.100 mg/L) in high methyl glyoxal environment inhibits precisely decreasing the mitogenic activity due to increasing the proliferation of MC3T3E1 pre-osteoblast cell ( 25 ). It seems caprine alpha-S2 casein protein have potency for bone loss therapeutic agent and prevent inflammation.…”

Section: Discussionmentioning

confidence: 99%

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

Fatchiyah¹,

Hardiyanti²,

Widodo³

2015

Acta Inform Med

Self Cite

View full text Add to dashboard Cite

Background:Advanced Glycation End Products (AGE) play a pivotal role in the development various degenerative diseases such as diabetes, cardiovascular disease, stroke, neuropathy, and nephropathy. Different studies have been done to employ AGEs as drug targets for the diseases therapy. In previous study, we have found bioactive peptide from Ethawah goat milk for anti-diabetic that may work through inhibition of AGE receptor function. However, the mechanism of bioactive peptides inhibits AGE- AGE receptor (RAGE) bonding still not clear yet. Therefore we investigated the inhibition mechanism by calculate the potential energy binding among the peptides, AGEs and RAGE using molecular docking system.Methods:Modeling 3D-structure was predicted by SWISS-MODEL web server. The virtual interaction was analyzed by docking system using HEX 8.0, Pymol and Discovery Studio 4.0 software.Results:this study showed that AGEs (Argypirimidine, Imidazole, Pentosidine and Pyrraline) bind to C-domain of RAGE. The total energy binding of RAGE with Argypirimidine, Imidazole, Pentosidine and Pyrraline were 378.35kJ/mol, -74.57kJ/mol, -301.25kJ/mol and -400.72kJ/mol, respectively. We have found three peptides among eight peptides from Ethawah goat milk, which are able bind to C-domain of RAGE, there are CSN1S2 f41-47, CSN1S2 f182-189, and CSN1S2 f214-221. The CSN1S22 f41-47 at arginine residue 47 interacts with proline162, leusine163 and leusine158 of RAGE. The total binding energy between CSN1S2 f41-47, CSN1S2 f182-189, and CSN1S2 f214-221 with RAGE were -378.35 kJ/mol, -359.97kJ/mol, -356.78 kJ/mol, respectively. Total binding energy and binding pattern indicated that RAGE more prefer bind with peptide and block AGE bind to functional site of RAGE. Further analysis showed that complex peptide-RAGE shifted binding site of AGE on function domain RAGE.Conclusion:This study suggested that the peptides from Ethawah goat milk may act as an inhibitor of AGEs-RAGE interaction that impaired signal transduction cascade at the cellular level.

show abstract

Section: Discussionmentioning

confidence: 99%

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

Fatchiyah¹,

Hardiyanti²,

Widodo³

2015

Acta Inform Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Milk and yogurt was taken from Ethawah breed goat milk, at UPTD Indonesian local goat, and Singosari, Malang. Isolation of milk and yogurt goat CSN1S2 protein was performed according to the previous study ( 12 ) with some modifications.…”

Section: Methodsmentioning

confidence: 99%

“…In vivo study was explained that the CSN1S2 protein act as an anti-inflammatory agent that could repair the villi ileum destruction ( 4 ) and reduce the pro-inflammatory cytokine in synovial rheumatoid arthritis rat ( 11 ). In vitro study shows that the CSN1S2 protein of goat milk can increase proliferation MC3T3E1 pre-osteoblast cells due to methylglyoxal exposure ( 12 ). Beside that, the modeling biological study was explained that the CSN1S2 protein act as an inhibitor of AGEs-RAGE interaction at cellular level( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Inhibition on JAK-STAT3 Signaling Transduction Cascade Is Taken by Bioactive Peptide Alpha-S2 Casein Protein from Goat Ethawah Breed Milk

Rohmah¹,

Hardiyanti²,

Fatchiyah³

2015

Acta Inform Med

Self Cite

View full text Add to dashboard Cite

Background:RA is a systemic inflammatory disease that causes developing comorbidity conditions. This condition can cause by overproduction of pro-inflammatory cytokine. In a previous study, we have found bioactive peptide CSN1S2 from Ethawah goat milk for anti-inflammatory for repair the ileum destruction. However, the signaling transduction cascade of bioactive peptides inhibits inflammation still not clear yet. Therefore, we analyzed the signaling transduction cascade via JAK-STAT3 pathway by in vivo and in silico.Methods:The ileum was isolated DNA and amplification with specific primer. The sequence was analyzed using the Sanger sequencing method. Modeling 3D-structure was predicted by SWISS-MODEL and virtual interaction was analyzed by docking system using Pymol and Discovery Studio 4.0 software.Results:This study showed that STAT3 has target gene 480bp. The normal group and normal treating- CSN1S2 of goat milk have similarity from gene bank. Whereas, RA group had transversion mutation that the purine change into pyrimidine even cause frameshift mutation. Interestingly, after treating with the CSN1S2 protein of goat milk shows reverse to the normal acid sequence group. Based on in silico study, from eight peptides, only three peptides of CSN1S2 protein, which carried by PePT1 to enter the small intestine. The fragments are PepT1-41-NMAIHPR-47; PepT1-182-KISQYYQK-189 and PepT1-214-TNAIPYVR-221. We have found just one bioactive peptide of f182-KISQYYQK-189 is able bind to STAT3. The energy binding of f182-KISQYYQK-189 and RA-STAT3 amino acid, it was Σ = -402.43 kJ/mol and the energy binding of f182-KISQYYQK-189 and RAS-STAT3 amino acid is decreasing into Σ = -407.09 kJ/mol.Conclusion:This study suggested that the fragment 182-KISQYYQK-189 peptides from Ethawah goat milk may act as an anti-inflammatory agent via JAK-STAT3 signal transduction cascade at the cellular level.

show abstract

“…This proves that CSN1S2 protein can induce proliferation of microglial cells in the CNS. CSN1S2 is known as an inducible molecule for proliferation 17 of pre-osteoblast cells that form the microstructure of the femur. 18 Protein CSN1S2 has not just the potential for proliferation of immunocompetent cells, however, it could provide potential nutrition for inflammation.…”

Section: -2mentioning

confidence: 99%

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

Rika

Fatchiyah

2017

AIP Conference Proceedings

View full text Add to dashboard Cite

The improvement of the quality of polluted irrigation water through a phytoremediation process in a hydroponic batch culture system AIP Conference Proceedings 1908, 030003 (2017) Abstract. Type-2 diabetes mellitus (T2DM) is a degenerative disease that causes an imbalance in the metabolism. The aim of this research is to determine the influences of CSN1S2 on the structure of microglial cells in T2DM. Rats (Rattus norvegicus) were divided into eight groups of treatment with looping three times each between treatment groups (CM) Control. The control is given a milk treatment with doses of 375 mg/kg (CM375), 750 mg/kg (CM750), and 1500 mg/kg (CM1500), T2DM (DMK), and T2DM with CSN1S2 375 mg/kg dose (DM375), 750mg/kg (DM750), and 1500 mg/kg (DM1500). The animal model T2DM was induced by a high-fat diet in the form of feed followed by injection of STZ (dose of 25 mg/kg of animal treatment) and treatment of CSN1S2 for 28 days. Brain organs were taken and analysed in histopathology stained by Hematoxylin-eosin (HE) and observed using Olympus BX53. Based on the results, it was concluded that CSN1S2 protein is influential for induction of microglial cell proliferation in animal models of T2DM, as immunity responds to the inflammatory condition in T2DM.

show abstract

CSN1S2 protein of goat milk inhibits the decrease of viability and increases the proliferation of MC3T3E1 pre-osteoblast cell in methyl glyoxal exposure

Cited by 13 publications

References 32 publications

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

Inhibition on JAK-STAT3 Signaling Transduction Cascade Is Taken by Bioactive Peptide Alpha-S2 Casein Protein from Goat Ethawah Breed Milk

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

Contact Info

Product

Resources

About