CTX-M-15 Extended-Spectrum β-Lactamase in a Shiga Toxin-Producing Escherichia coli Isolate of Serotype O111:H8

Valat, Charlotte; Haenni, Marisa; Saras, Estelle; Auvray, Frédéric; Forest, Karine; Oswald, Éric; Madec, Jean‐Yves

doi:10.1128/aem.06997-11

Cited by 34 publications

(22 citation statements)

References 22 publications

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“…Subtyping of stx 1 and stx 2 genes was performed as described previously (33). The presence of additional EHEC virulence markers (ehxA gene and OI-122-associated genes, namely, pagC, nleB, and efa1 genes) was screened by PCR as described previously (34,35). The presence of the virulence marker espK was screened by PCR for STEC O26:H11 (36).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Bibbal

Loukiadis

Kérourédan

et al. 2015

Appl Environ Microbiol

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The main pathogenic enterohemorrhagic Escherichia coli (EHEC) strains are defined as Shiga toxin (Stx)-producing E. coli (STEC) belonging to one of the following serotypes: O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28. Each of these five serotypes is known to be associated with a specific subtype of the intimin-encoding gene (eae). The objective of this study was to evaluate the prevalence of bovine carriers of these "top five" STEC in the four adult cattle categories slaughtered in France. Fecal samples were collected from 1,318 cattle, including 291 young dairy bulls, 296 young beef bulls, 337 dairy cows, and 394 beef cows. A total of 96 E. coli isolates, including 33 top five STEC and 63 atypical enteropathogenic E. coli (aEPEC) isolates, with the same genetic characteristics as the top five STEC strains except that they lacked an stx gene, were recovered from these samples. O157:H7 was the most frequently isolated STEC serotype. The prevalence of top five STEC (all serotypes included) was 4.5% in young dairy bulls, 2.4% in young beef bulls, 1.8% in dairy cows, and 1.0% in beef cows. It was significantly higher in young dairy bulls (P < 0.05) than in the other 3 categories. The basis for these differences between categories remains to be elucidated. Moreover, simultaneous carriage of STEC O26:H11 and STEC O103:H2 was detected in one young dairy bull. Lastly, the prevalence of bovine carriers of the top five STEC, evaluated through a weighted arithmetic mean of the prevalence by categories, was estimated to 1.8% in slaughtered adult cattle in France.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Bibbal

Loukiadis

Kérourédan

et al. 2015

Appl Environ Microbiol

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“…The espP, eae, stx 1 , and stx 2 genes were detected as described previously (10,13,22) by using the primers listed in Table 1. eae variants and the genomic island OI-122 were detected by real-time PCR (29,47). A subset of 36 E. coli isolates from the A (13 isolates), B1 (10 isolates), and D (13 isolates) phylogroups that were positive for the eae, stx 1 , and/or espP gene was randomly sampled and was subjected to DNA microarray analysis (Identibac EC, Alere, France) for the presence of stx 1 , stx 2 , eae, and 38 additional virulence factors (VFs) (see Table 4).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Grouping and Virulence Potential of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains in Cattle

Valat¹,

Auvray

Forest³

et al. 2012

Appl Environ Microbiol

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ABSTRACTIn line with recent reports of extended-spectrum beta-lactamases (ESBLs) inEscherichia coliisolates of highly virulent serotypes, such as O104:H4, we investigated the distribution of phylogroups (A, B1, B2, D) and virulence factor (VF)-encoding genes in 204 ESBL-producingE. coliisolates from diarrheic cattle. ESBL genes, VFs, and phylogroups were identified by PCR and a commercial DNA array (Alere, France). ESBL genes belonged mostly to the CTX-M-1 (65.7%) and CTX-M-9 (27.0%) groups, whereas those of the CTX-M-2 and TEM groups were much less represented (3.9% and 3.4%, respectively). One ESBL isolate wasstx1andeaepositive and belonged to a major enterohemorrhagicE. coli(EHEC) serotype (O111:H8). Two other isolates wereeaepositive butstxnegative; one of these had serotype O26:H11. ESBL isolates belonged mainly to phylogroup A (55.4%) and, to lesser extents, to phylogroups D (25.5%) and B1 (15.6%), whereas B2 strains were quasi-absent (1/204). The number of VFs was significantly higher in phylogroup B1 than in phylogroups A (P= 0.04) and D (P= 0.02). Almost all of the VFs detected were found in CTX-M-1 isolates, whereas only 64.3% and 33.3% of them were found in CTX-M-9 and CTX-M-2 isolates, respectively. These results indicated that the widespread dissemination of theblaCTX-Mgenes within theE. colipopulation from cattle still spared the subpopulation of EHEC/Shiga-toxigenicE. coli(STEC) isolates. In contrast to other reports on non-ESBL-producing isolates from domestic animals, B1 was not the main phylogroup identified. However, B1 was found to be the most virulent phylogroup, suggesting host-specific distribution of virulence determinants among phylogenetic groups.

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“…This ␤-lactamase and other CTX-M-type enzymes are also prevalent in other virulent E. coli clones, such as Shiga toxinproducing serotype O111:H8 (27) and O26:H11 (28), and even in E. coli strains isolated from chicken and pig farms in Spain, highlighting the potential risk of zoonotic transmission (29). Thus, virulence factors have been identified in E. coli isolates that produce CTX-M-type ESBL (30), and E. coli ST131, which produces the widespread NDM-1 enzyme, has been found to exhibit a wide array of virulence factors (31).…”

Section: Ribosomal Protection Tet44mentioning

confidence: 99%

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

Beceiro

Tomás²,

Bou³

2013

Clin Microbiol Rev

855

630

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SUMMARY Hosts and bacteria have coevolved over millions of years, during which pathogenic bacteria have modified their virulence mechanisms to adapt to host defense systems. Although the spread of pathogens has been hindered by the discovery and widespread use of antimicrobial agents, antimicrobial resistance has increased globally. The emergence of resistant bacteria has accelerated in recent years, mainly as a result of increased selective pressure. However, although antimicrobial resistance and bacterial virulence have developed on different timescales, they share some common characteristics. This review considers how bacterial virulence and fitness are affected by antibiotic resistance and also how the relationship between virulence and resistance is affected by different genetic mechanisms (e.g., coselection and compensatory mutations) and by the most prevalent global responses. The interplay between these factors and the associated biological costs depend on four main factors: the bacterial species involved, virulence and resistance mechanisms, the ecological niche, and the host. The development of new strategies involving new antimicrobials or nonantimicrobial compounds and of novel diagnostic methods that focus on high-risk clones and rapid tests to detect virulence markers may help to resolve the increasing problem of the association between virulence and resistance, which is becoming more beneficial for pathogenic bacteria.

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CTX-M-15 Extended-Spectrum β-Lactamase in a Shiga Toxin-Producing Escherichia coli Isolate of Serotype O111:H8

Cited by 34 publications

References 22 publications

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Phylogenetic Grouping and Virulence Potential of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains in Cattle

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

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