Cu–hemin metal-organic frameworks with peroxidase-like activity as peroxidase mimics for colorimetric sensing of glucose

Liu, Fenfen; He, Juan; Zeng, Mulang; Hao, Jie; Guo, Qiaohui; Song, Yonghai; Wang, Li

doi:10.1007/s11051-016-3416-z

Cited by 80 publications

(28 citation statements)

References 46 publications

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“…The pores of MOFs provide incorporating of GOD molecules into Cu-hemin MOFs which effectively avoided the aggregation of enzyme on the surface of electrode. In addition, Cu-hemin MOFs can be employ to colorimetric detection of H 2 O 2 and glucose [ 26 ]. Nevertheless, there have been a few studies on usage of the intrinsic enzyme-like activities of MOF for biomedical applications such as cancer diagnostic [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Ni-hemin metal–organic framework with highly efficient peroxidase catalytic activity: toward colorimetric cancer cell detection and targeted therapeutics

et al. 2018

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BackgroundGiven the great benefits of artificial enzymes, a simple approach is proposed via assembling of Ni2+ with hemin for synthesis of Ni-hemin metal–organic-frameworks (Ni-hemin MOFs) mimic enzyme. The formation of the Ni-hemin MOFs was verified by scanning electron microscopy, Transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Energy-dispersive X-ray spectroscopy and UV–vis absorption spectroscopy. This novel nanocomposite exhibited surprising peroxidase like activity monitored by catalytic oxidation of a typical peroxidase substrate, 3,3,5,5′-tetramethylbenzidine, in the presence of H2O2. By using folic acid conjugated MOF nanocomposite as a recognition element, we develop a colorimetric assay for the direct detection of cancer cells.ResultsThe proposed sensor presented high sensitivity and selectivity for the detection of human breast cancer cells (MCF-7) and Human Caucasian gastric adenocarcinoma. By measuring UV–vis absorbance response, a wide detection range from 50 to 105 cells/mL with a detection limit as low as 10 cells/mLwas reached for MCF-7 cells. We further discuss therapeutics efficiency of Ni-hemin MOFs in the presence of H2O2 and ascorbic acid. Peroxidase-mimic Ni-hemin MOFs as reactive oxygen species which could damage MCF-7 cancer cells, however for normal cells (human embryonic kidney HEK 293 cells) killing effect was negligible.ConclusionsBased on these behaviors, the developed method offers a fast, easy and cheap assay for the interest in future diagnostic and treatment application.

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Section: Introductionmentioning

confidence: 99%

Ni-hemin metal–organic framework with highly efficient peroxidase catalytic activity: toward colorimetric cancer cell detection and targeted therapeutics

et al. 2018

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“…As mentioned above, MOFs that possess catalytically active metal sites and high porosity have exhibited enzyme‐mimetic activities, such as oxidase (OXD), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). [ 67–69 ] To date, a variety of transition‐metal active centers, such as Fe, Cu, and Zr, as well as organic ligands such as terephthalic acid (H 2 BDC) and 1,3,5‐benzenetricarboxylic acid (H 3 BTC) have been used to fabricate diverse structural and nanosized Enz‐Cats with the desired catalytic activity. Featuring the POD‐like activities, Materials Institute Lavoisier (MIL)‐type MOFs, including MIL‐53, MIL‐88, MIL‐100, and MIL‐101, were used for the detection of H 2 O 2 , [ 70 ] uric acid, [ 71 ] glucose, [ 72,73 ] dopamine, [ 74 ] biomolecules, [ 75 ] and cancer cells, [ 76 ] which could be attributed to hydroxyl radical (·OH)‐induced color changes via catalytic activation of H 2 O 2 .…”

Section: Engineering Enz‐cats From Pristine Mofs and Modified Mofsmentioning

confidence: 99%

Metal–Organic‐Framework‐Engineered Enzyme‐Mimetic Catalysts

et al. 2020

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high sensitivity to surroundings, and difficulties in recycling and reuse, limit their further applications. Thus, this situation has promoted the rapid exploration and development of various artificial enzyme mimics, including fullerenes, porphyrins, biomolecules, metal complexes, polymers, and functional nanomaterials. [3-6] Among these enzyme-mimetic catalysts (Enz-Cats, also defined as nanozymes in some systems), [4,5,7,8] alkaline metals, transition metals, and lanthanoid components [9-11] have been devoted to extending their applications in the fields of disease diagnosis, [12,13] wound disinfection, [14] and tumor treatments. [15-17] In particular, synthesizing metal-based nanomaterials to engineer Enz-Cats by precisely modulating their catalytic metal centers, sizes, structures, porosities, and compositions has been a long-standing objective, which provides tremendous opportunities to explore highly efficient Enz-Cats and reveal the essential catalytic mechanisms. [18-21] Although some developed Enz-Cats have exhibited efficient in vitro activities, the catalytic performances and selectivity in diverse physiological environments are still vital considerations during the exploration of their further application in biomedical areas. [22-24] Furthermore, the inherent physicochemical characteristics of these Enz-Cats may yield multiple catalytic reactions, such as generating or scavenging reactive oxygen species (ROS) under internal microenvironments Nanomaterial-based enzyme-mimetic catalysts (Enz-Cats) have received considerable attention because of their optimized and enhanced catalytic performances and selectivities in diverse physiological environments compared with natural enzymes. Recently, owing to their molecular/atomic-level catalytic centers, high porosity, large surface area, high loading capacity, and homogeneous structure, metal-organic frameworks (MOFs) have emerged as one of the most promising materials in engineering Enz-Cats. Here, the recent advances in the design of MOF-engineered Enz-Cats, including their preparation methods, composite constructions, structural characterizations, and biomedical applications, are highlighted and commented upon. In particular, the performance, selectivities, essential mechanisms, and potential structure-property relations of these MOF-engineered Enz-Cats in accelerating catalytic reactions are discussed. Some potential biomedical applications of these MOF-engineered Enz-Cats are also breifly proposed. These applications include, for example, tumor therapies, bacterial disinfection, tissue regeneration, and biosensors. Finally, the future opportunities and challenges in emerging research frontiers are thoroughly discussed. Thereby, potential pathways and perspectives for designing future state-of-the-art Enz-Cats in biomedical sciences are offered.

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“…Also, peroxidases (enzymes that catalyze oxidation reactions) are popular detection tools because they simplify colour changes in the presence of certain dyes. For example, immune-absorbent assay ELIZA tests [55]. They are also useful in the treatment of waste water for oxidizing organic substances [56].…”

Section: Iron Oxide-based Nanomaterialsmentioning

confidence: 99%

Nanozyme applications in biology and medicine: an overview

Golchin

Alidadian

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

117

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Nanozymes, in nature, are artificial enzymes. Innovated by Ronald Breslow to mimic enzymes. Nanozymes have widespread applications including targeted cancer therapy, diagnostic medicine and bio-sensing even environmental toxicology. However, these applications are a novel research field in biomedicine, but are growing fast. Enzyme-based applications such as immune-absorbent assay (ELIZA) are expensive because of the complexity of producing enzymes and antibodies. Not only, some nanoparticles can mimic these enzymes such as superoxides, but also they can manipulate biological pathways directly like autophagy. These abilities make them a suitable alternative for both therapy and diagnosis. In this review, we opted on metal nanoparticles and application of this cutting edged technology into modern medicine.

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Cu–hemin metal-organic frameworks with peroxidase-like activity as peroxidase mimics for colorimetric sensing of glucose

Cited by 80 publications

References 46 publications

Ni-hemin metal–organic framework with highly efficient peroxidase catalytic activity: toward colorimetric cancer cell detection and targeted therapeutics

Ni-hemin metal–organic framework with highly efficient peroxidase catalytic activity: toward colorimetric cancer cell detection and targeted therapeutics

Metal–Organic‐Framework‐Engineered Enzyme‐Mimetic Catalysts

Nanozyme applications in biology and medicine: an overview

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