Cubosomes for topical delivery of the antimicrobial peptide LL-37

Boge, Lukas; Hallstensson, K.; Ringstad, Lovisa; Johansson, Jenny; Andersson, Therése; Davoudi, Mina; Larsson, Per Tomas; Mahlapuu, Margit; Håkansson, Joakim; Andersson, Martin

doi:10.1016/j.ejpb.2018.11.009

Cited by 151 publications

(101 citation statements)

References 54 publications

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“…LL-37 loaded into cubosomes was protected against proteolytic degradation by P. aeruginosa elastase while improving AMP bioavailability and efficiency. In an ex vivo wound infection model, LL-37-loaded cubosomes showed the highest killing efficiency of S. aureus and an absence of pig skin irritation [154]. Further molecular dynamic simulations confirmed these experimental results, revealing that charged amine and guanidium groups from LL-37 are responsible for facilitating the interaction with the bacterial membrane.…”

Section: Polymeric Nanoparticles Nanoemulsions and Micellessupporting

confidence: 52%

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

2020

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Bacterial infections occur when wound healing fails to reach the final stage of healing, which is usually hindered by the presence of different pathogens. Different topical antimicrobial agents are used to inhibit bacterial growth due to antibiotic failure in reaching the infected site, which is accompanied very often by increased drug resistance and other side effects. In this review, we focus on antimicrobial peptides (AMPs), especially those with a high potential of efficacy against multidrug-resistant and biofilm-forming bacteria and fungi present in wound infections. Currently, different AMPs undergo preclinical and clinical phase to combat infection-related diseases. AMP dendrimers (AMPDs) have been mentioned as potent microbial agents. Various AMP delivery strategies that are used to combat infection and modulate the healing rate—such as polymers, scaffolds, films and wound dressings, and organic and inorganic nanoparticles—have been discussed as well. New technologies such as Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated protein (CRISPR-Cas) are taken into consideration as potential future tools for AMP delivery in skin therapy.

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Section: Polymeric Nanoparticles Nanoemulsions and Micellessupporting

confidence: 52%

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

2020

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“…Various kinds of self-assembled nonlamellar liquid crystalline nanoparticles have been recently evaluated for their potential as drug delivery systems for antimicrobial molecules [176]. The system consists of amphiphiles that orient themselves into different mesophase structures, for example hexagonal, lamellar, cubic, and the less common sponge structure [177,178]. Advantages of these nanosystems include their amphiphilic nature and their larger interfacial surface areas, which enable them to encapsulate both hydrophilic and hydrophobic drugs, in addition to the ease of production with the ability to scale-up.…”

Section: Nonlamellar Lyotropic Liquid Crystalline Nanoparticlesmentioning

confidence: 99%

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

et al. 2020

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Based on the recent reports of World Health Organization, increased antibiotic resistance prevalence among bacteria represents the greatest challenge to human health. In addition, the poor solubility, stability, and side effects that lead to inefficiency of the current antibacterial therapy prompted the researchers to explore new innovative strategies to overcome such resilient microbes. Hence, novel antibiotic delivery systems are in high demand. Nanotechnology has attracted considerable interest due to their favored physicochemical properties, drug targeting efficiency, enhanced uptake, and biodistribution. The present review focuses on the recent applications of organic (liposomes, lipid-based nanoparticles, polymeric micelles, and polymeric nanoparticles), and inorganic (silver, silica, magnetic, zinc oxide (ZnO), cobalt, selenium, and cadmium) nanosystems in the domain of antibacterial delivery. We provide a concise description of the characteristics of each system that render it suitable as an antibacterial delivery agent. We also highlight the recent promising innovations used to overcome antibacterial resistance, including the use of lipid polymer nanoparticles, nonlamellar liquid crystalline nanoparticles, anti-microbial oligonucleotides, smart responsive materials, cationic peptides, and natural compounds. We further discuss the applications of antimicrobial photodynamic therapy, combination drug therapy, nano antibiotic strategy, and phage therapy, and their impact on evading antibacterial resistance. Finally, we report on the formulations that made their way towards clinical application.

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“…Temporary eradication of S. aureus with antibiotics often leads to clinical improvement. Antimicrobial resistance is one of the biggest challenges in the global health sector [ 20 ]. The high incidence of methicillin resistance in hospitals complicates the prevention and treatment of serious infections due to staphylococci [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing

et al. 2020

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The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2/h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.

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Cubosomes for topical delivery of the antimicrobial peptide LL-37

Cited by 151 publications

References 54 publications

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

Nanomedicine Fight against Antibacterial Resistance: An Overview of the Recent Pharmaceutical Innovations

Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing

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