Cucurbitacins from the Leaves of  Citrullus colocynthis (L.) Schrad

Chawech, Rachid; Jarraya, Raoudha; Girardi, Cynthia; Vansteelandt, Marieke; Marti, Guillaume; Nasri, Imen; Racaud‐Sultan, Claire; Fabre, Nicolás

doi:10.3390/molecules201018001

Cited by 37 publications

(28 citation statements)

References 38 publications

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“…A detailed phytochemical characterization of the leaves, stems, and roots of C. colocynthis revealed the presence of different classes of metabolites—such as coumarins, hydroxycinnamic acid derivatives, flavan-3-ols glycosides, flavone glycosides, and tetracyclic triterpenes (Table 1)—as according to previous investigations [42,43,44]. These compounds, which have been indicated as responsible for antifungal activity against Aspergillus strains [38], were abundant in the analyzed extracts (Table 1).…”

Section: Resultssupporting

confidence: 79%

Aspergillus flavus as a Model System to Test the Biological Activity of Botanicals: An Example on Citrullus colocynthis L. Schrad. Organic Extracts

Degola

Marzouk

Gori

et al. 2019

Toxins

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Citrullus colocynthis L. Schrader is an annual plant belonging to the Cucurbitaceae family, widely distributed in the desert areas of the Mediterranean basin. Many pharmacological properties (anti-inflammatory, anti-diabetic, analgesic, anti-epileptic) are ascribed to different organs of this plant; extracts and derivatives of C. colocynthis are used in folk Berber medicine for the treatment of numerous diseases—such as rheumatism arthritis, hypertension bronchitis, mastitis, and even cancer. Clinical studies aimed at confirming the chemical and biological bases of pharmacological activity assigned to many plant/herb extracts used in folk medicine often rely on results obtained from laboratory preliminary tests. We investigated the biological activity of some C. colocynthis stem, leaf, and root extracts on the mycotoxigenic and phytopathogenic fungus Aspergillus flavus, testing a possible correlation between the inhibitory effect on aflatoxin biosynthesis, the phytochemical composition of extracts, and their in vitro antioxidant capacities.

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Section: Resultssupporting

confidence: 79%

Aspergillus flavus as a Model System to Test the Biological Activity of Botanicals: An Example on Citrullus colocynthis L. Schrad. Organic Extracts

Degola

Marzouk

Gori

et al. 2019

Toxins

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show abstract

“…These results were quite close and within the range of the values specified by the suppliers [37,38]. [40][41][42][43][44][45]. The HPLC method was used to quantitatively determine the amount of Cu D and Cu I isolated from the fruit juice.…”

Section: Resultssupporting

confidence: 71%

Simultaneous isolation and purification of cucurbitacin D and I from Ecballium elaterium (l.) A. Rich fruit juice

Tosun

Baysar

2019

Maced. J. Chem. Chem. Eng.

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The objective of this study was to develop a rapid, economic, and efficient method for simultaneous selective isolation, separation, and purification of cucurbitacin D and I from Ecballium elaterium (L.) A. Rich fruit juice via reversed-phase flash chromatography combined with HPLC. The chloroform extract of the fruit juice was fractionated with flash chromatography using a chloroform, acetone and methanol solvent combination at a 5 ml/min flow rate. Then, a validated HPLC method was utilized for purification of the two targeted cucurbitacins. Cucurbitacin D and I were collected automatically by the fraction collector. The fractions containing the same compounds were pooled and lyophilized. The purified cucurbitacin D and I compounds were identified by NMR, LC-MS, and UV spectra analysis. The results suggest that the applied procedure is simple, quick, and highly efficient. The HPLC method was found to be linear, accurate, precise and rugged for the quantification of the cucurbitacins studied.

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“…Studies have shown that cucurbitacins causes marked changes in the actin cytoskeleton. Cucurbitacin is a class of tetracyclic triterpenoids extracted from the cucurbitaceae that have a wide range of pharmacological effects, such as anti-inflammatory, anti-cancer, and liver protective effects ( Chen et al, 2012 ; Recio et al, 2012 ; Cai et al, 2015 ; Chawech et al, 2015 ). Among the numerous known family members of cucurbitacin, CuE is one of the more important members.…”

Section: Discussionmentioning

confidence: 99%

Cucurbitacin E Inhibits Proliferation and Migration of Intestinal Epithelial Cells via Activating Cofilin

Song

Wang

et al. 2018

Front. Physiol.

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The proliferation and migration of intestinal epithelial cell is important to the barrier integrity of intestinal epithelium. Cucurbitacin E (CuE) is one of the tetracyclic triterpenoids extracted from the cucurbitaceae that has been shown to inhibit cancer cell growth, tumor angiogenesis and inflammatory response. Nevertheless, the role of Cucurbitacin E in regulating the proliferation and migration of intestinal epithelial cells remain unclear. In this study, the human intestinal epithelial cell line Caco-2 was treated with CuE and the effects of CuE on cell cycle, proliferation, migration and actin dynamics in Caco-2 cells were investigated successively. We found that CuE significantly inhibited the cell proliferation and migration, inducing the cell cycle arrest in G2/M phase and disrupting the actin dynamic balance in Caco-2 cells. Finally, we showed that CuE inhibited cofilin phosphorylation by suppressing the phosphorylation of both LIM kinase (LIMK)1 and LIMK2 in vitro, resulting in the activation of cofilin, which is closely associated with cell proliferation and migration. Therefore, our studies provided the first evidence that CuE inhibited the proliferation and migration of intestinal epithelial cells via activating cofilin, and CuE is a potential candidate in intestinal disease therapy.

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Cucurbitacins from the Leaves of Citrullus colocynthis (L.) Schrad

Cited by 37 publications

References 38 publications

Aspergillus flavus as a Model System to Test the Biological Activity of Botanicals: An Example on Citrullus colocynthis L. Schrad. Organic Extracts

Aspergillus flavus as a Model System to Test the Biological Activity of Botanicals: An Example on Citrullus colocynthis L. Schrad. Organic Extracts

Simultaneous isolation and purification of cucurbitacin D and I from Ecballium elaterium (l.) A. Rich fruit juice

Cucurbitacin E Inhibits Proliferation and Migration of Intestinal Epithelial Cells via Activating Cofilin

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