Culex flavivirus infection in a Culex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus

Talavera, Sandra; Birnberg, Lotty; Núñez, Ana Isabel; Muñoz‐Muñoz, Francesc; Vázquez, Ana; Busquets, Núria

doi:10.1186/s13071-018-2887-4

Cited by 36 publications

(23 citation statements)

References 54 publications

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“…In vivo studies showed a delay of dissemination from the midgut by 3 days 2015 [ 31 ] Hall-Mendelin et al PCV No Yes WNV Significantly lower infection rate in PCV-infected Cx. Annulirostris orally exposed to WNV 2016 [ 98 ] Talavera et al CxFV No Yes RFV No effect 2018 [ 94 ] Schultz et al CFAV, PCLV Yes No ZIKV, DENV & LACV 90% reduction of ZIKV & DENV growth. Complete inhibition of LACV at MOI 0.1 2018 [ 104 ] …”

Section: Vector Competence Studiesmentioning

confidence: 99%

Insect-specific virus evolution and potential effects on vector competence

2019

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The advancement in high-throughput sequencing technology and bioinformatics tools has spurred a new age of viral discovery. Arthropods is the largest group of animals and has shown to be a major reservoir of different viruses, including a group known as insect-specific viruses (ISVs). The majority of known ISVs have been isolated from mosquitoes and shown to belong to viral families associated with animal arbovirus pathogens, such as Flaviviridae, Togaviridae and Phenuiviridae . These insect-specific viruses have a strict tropism and are unable to replicate in vertebrate cells, these properties are interesting for many reasons. One is that these viruses could potentially be utilised as biocontrol agents using a similar strategy as for Wolbachia . Mosquitoes infected with the viral agent could have inferior vectorial capacity of arboviruses resulting in a decrease of circulating arboviruses of public health importance. Moreover, insect-specific viruses are thought to be ancestral to arboviruses and could be used to study the evolution of the switch from single-host to dual-host. In this review, we discuss new discoveries and hypothesis in the field of arboviruses and insect-specific viruses.

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Section: Vector Competence Studiesmentioning

confidence: 99%

Insect-specific virus evolution and potential effects on vector competence

2019

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“…Part of the uncovered microbiome is the insect-specific viruses (ISVs), viruses restricted to arthropods that are unable to replicate in vertebrate cells [27]. Insect-specific viruses are highly prevalent in wild mosquito populations [25,[28][29][30][31] and have been reported to suppress [32][33][34][35][36], enhance [37,38], or have no effect [39] on replication of medically important arboviruses, potentially affecting vector competence [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of Phasi Charoen-like virus from wild-caught Aedes aegypti in Grenada, W.I. as revealed by metagenomic analysis

et al. 2020

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Arboviruses cause diseases of significant global health concerns. Interactions between mosquitoes and their microbiota as well as the important role of this interaction in the mosquito's capacity to harbor and transmit pathogens have emerged as important fields of research. Aedes aegypti is one of the most abundant mosquitoes in many geographic locations, a vector capable of transmitting a number of arboviruses such as dengue and Zika. Currently, there are few studies on the metavirome of this mosquito particularly in the Americas. This study analyzes the metavirome of A. aegypti from Grenada, a Caribbean nation with tropical weather, abundant A. aegypti, and both endemic and arboviral pathogens transmitted by this mosquito. Between January and December 2018, 1152 mosquitoes were collected from six semi-rural locations near houses in St. George Parish, Grenada, by weekly trapping using BG-Sentinel traps. From these, 300 A. aegypti were selected for analysis. The metavirome was analyzed using the Illumina HiSeq 1500 for deep sequencing. The generation sequencing library construction protocol used was NuGEN Universal RNA with an average read length of 125 bp. Reads were mapped to the A. aegypti assembly. Non-mosquito reads were analyzed using the tools FastViromeExplorer. The NCBI total virus, RNA virus, and eukaryotic virus databases were used as references. The metagenomic comparison analysis showed that the most abundant virus-related reads among all databases and assemblies was Phasi Charoen-like virus. The Phasi Charoen-like virus results are in agreement to other studies in America, Asia and Australia. Further studies using wild-caught mosquitoes is needed to assess the impact of this insect-specific virus on the A. aegypti lifecycle and vector capacity.

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“…quinquefasciatus co-infected with CxFV Izabal [60] and CxFV did not impact RVFV infection in Cx. pipiens mosquitoes [61]. Co-infection studies using cell-fusing agent virus (CFAV, Flavivirus) and dengue virus (DENV) in Aag2 cells shows mutual interaction through activation of Ribonuclease K (AeRNASEK) expression [14], whereas in Ae.…”

Section: Discussionmentioning

confidence: 99%

Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes

Parry

Naccache

Ndiaye

et al. 2020

Viruses

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The inland floodwater mosquito Aedes vexans (Meigen, 1830) is a competent vector of numerous arthropod-borne viruses such as Rift Valley fever virus (Phenuiviridae) and Zika virus (Flaviviridae). Aedes vexans spp. have widespread Afrotropical distribution and are common European cosmopolitan mosquitoes. We examined the virome of Ae. vexans arabiensis samples from Barkédji village, Senegal, with small RNA sequencing, bioinformatic analysis, and RT-PCR screening. We identified a novel 9494 nt iflavirus (Picornaviridae) designated here as Aedes vexans iflavirus (AvIFV). Annotation of the AvIFV genome reveals a 2782 amino acid polyprotein with iflavirus protein domain architecture and typical iflavirus 5’ internal ribosomal entry site and 3’ poly-A tail. Aedes vexans iflavirus is most closely related to a partial virus sequence from Venturia canescens (a parasitoid wasp) with 56.77% pairwise amino acid identity. Analysis of AvIFV-derived small RNAs suggests that AvIFV is targeted by the exogenous RNA interference pathway but not the PIWI-interacting RNA response, as ~60% of AvIFV reads corresponded to 21 nt Dicer-2 virus-derived small RNAs and the 24–29 nt AvIFV read population did not exhibit a “ping-pong” signature. The RT-PCR screens of archival and current (circa 2011–2020) Ae. vexans arabiensis laboratory samples and wild-caught mosquitoes from Barkédji suggest that AvIFV is ubiquitous in these mosquitoes. Further, we screened wild-caught European Ae. vexans samples from Germany, the United Kingdom, Italy, and Sweden, all of which tested negative for AvIFV RNA. This report provides insight into the diversity of commensal Aedes viruses and the host RNAi response towards iflaviruses.

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Culex flavivirus infection in a Culex pipiens mosquito colony and its effects on vector competence for Rift Valley fever phlebovirus

Cited by 36 publications

References 54 publications

Insect-specific virus evolution and potential effects on vector competence

Insect-specific virus evolution and potential effects on vector competence

High prevalence of Phasi Charoen-like virus from wild-caught Aedes aegypti in Grenada, W.I. as revealed by metagenomic analysis

Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes

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