The age of knowledge has brought new values, new ways of relating, doing business, learning. Public organizations need to adapt to this new reality and review their processes. Thus, this article aims to analyze the factors that influence the creation of organizational knowledge and innovation in the public sector. For that, it was adopted the methodology of a case study in a federal teaching institution. As a research tool, it was used a semi-structured interview script. The results of this study showed that the factors of commitment and teamwork are evaluated positively. In addition, there are flaws in factors such as communication and interorganizational network. Because it is a case study, this research presents as a limitation the impossibility of the results generalization, nevertheless it can serve as an input for other institutions whose focus is the progress of knowledge, creation and innovation. This article aims to contribute to scientific knowledge in the field of innovation in public administration, highlighting the importance of continuous improvement.