Cultural and Morphological Characterization of Trichoderma spp. Associated with Green Mold Disease of Pleurotus spp. in Kashmir

Shah, Shaiesta; Nasreen, Sahera; Sheikh, P. A.

doi:10.3923/jm.2012.139.144

Cited by 40 publications

(37 citation statements)

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“…Phialides were hung like banana in the conidiophore, base and apex were more narrow than middle. All these descriptions are in agreement with the findings of earlier investigators (Bisset, 1991aand b, Samuals et al, 1996, Gams and Bissett, 1998, Samuels et al, 2002, Samuels et al, 2002a, Choi et al, 2003, and Shah et al, 2012 who recorded the similar observations.…”

Section: Discussionsupporting

confidence: 82%

Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma

Sharma

Singh

2014

JANS

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Abstract:The genus Trichoderma contains species that are of a great economic importance due to their ability to act as biological control agents against a large variety of fungal plant pathogens. In the present investigation thirty isolates of the Trichoderma sp. were obtained from the rhizosphere soils of different plants at different locations at Nainital, Almora, Udham Singh Nagar, Derhadun, Haridwar and Tehri Garhwal districts of Uttarakhand (India). The isolates were characterized on the basis of their cultural and morphological characteristics. The cultural characteristics included linear growth, colony colour, pigmentation and growth pattern. Morphological characteristics studied were structure, shape and arrangement of conidiophores, phialides and conidia. Out of thirty isolates, 6 isolates namely PB10, PB13, PB23, PB26, PB27 and PB28 were identified as T. virens and remaining 24 isolates as T. harzianum.

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Section: Discussionsupporting

confidence: 82%

Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma

Sharma

Singh

2014

JANS

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“…The antagonist that successfully isolated out was identified as a Trichoderma sp.. The green colony characteristics observed in the present study were in agreement with those described in the studies by Shah et al (2012). The branching conidiophore and round and green colour conidia of the isolated Trichoderma sp.…”

Section: Discussionsupporting

confidence: 91%

“…Identification of the isolated Trichoderma sp. was based on the description by Shah et al (2012) by observing the macro-and microscopic structures of the fungus. The pure culture of Trichoderma sp.…”

Section: Isolation Of Antagonistic Fungusmentioning

confidence: 99%

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Effect of Trichoderma sp. on Anthracnose Disease of Stored Chilli

Yan¹,

Thanh²

2018

BJRST

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Chilli is commonly used as spice in Malaysian culinary, principal ingredients in paste (sambal) and as the raw material in sauce industry. Anthracnose disease caused by Colletotrichum capsici is one of the major causes of economic loss to chilli production especially in Asia. Even a small lesion on chilli might affect the quality, thus the market value of the chilli. Disease symptoms caused by C. capsici include brown, circular and sunken lesion with concentric rings of black acervuli. Chemicals have been used to treat the chilli but they might cause environmental pollution, affect human health and lead to pathogen resistance to the chemicals. Therefore, an alternative method to chemical control is required. In this study, C. capsici was isolated from a naturally infected chilli fruit (Capsicum frutescens), and a species of Trichoderma was isolated from the rhizosphere of grasses. Pure cultures of both fungi were established then used in antagonism studies in in vitro and in vivo. Dual culture of pathogens and Trichoderma sp. indicated that Trichoderma sp. competed with C. capsici for space and nutrients, caused the loss of turgidity of the fungal hyphae, and reduced the fungal growth by producing volatile metabolites. Trichoderma sp. decreased disease severity on chilli artificially inoculated fruits up to 64% when Trichoderma mycelial plug was used and 55% when culture filtrate was applied. Field trials are recommended to examine the antagonism of Trichoderma sp. in real production conditions. Keywords: Anthracnose, biological control, Colletotrichum capsici, Trichoderma sp.

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“…The endophytic Trichoderma ET537 isolate appearance was more cottony than endophytic Trichoderma ET523 and ET501 isolates. The same result was also reported by Shah et al (2012) where some of Trichoderma species showed white cottony mycelium with green conidiation towards the margins. The colour of mature conidia of endophytic Trichoderma ET501 isolate was yellowish green (Figure 2a), while conidia of endophytic Trichoderma ET523 and ET537 were greyish green colour (Figures 2b and 2c, respectively).…”

Section: Poison Food Agar Assaysupporting

confidence: 87%

THE POTENTIAL OF ENDOPHYTIC Trichoderma FROM OIL PALM (Elaeis guineensis Jacq.) ROOTS OF NORTH SUMATRA, INDONESIA AGAINST Ganoderma boninense

Haryadi¹

2019

JOPR

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Utilisation of endophyticTrichoderma increases tremendously as an alternative control against G. boninense, causal pathogen of basal stem rot (BSR) disease of oil palm. However, investigation of endophytic Trichoderma from Indonesia is still very scarce. The aims of this study were to isolate, identify and investigate the potential of endophytic Trichoderma from oil palm roots. Three potential endophytic Trichoderma species were isolated and further identified using macroscopic, microscopic, and molecular methods. Antagonistic activities of endophytic Trichoderma were tested using dual culture agar and poison food agar assay. A molecular approach using DNA sequencing of 5.8S-ITS region successfully identified the endophytic Trichoderma isolate ET501 as Trichoderma reesei strain RHa, while isolates of endophytic ET523 and ET537 were identified as Trichoderma asperellum isolate F1 and Trichoderma asperellum strain Q1, respectively. Trichoderma reesei ET501 was the most aggressive isolate against G. boninense with PIRG of 95.1% compared to T. asperellum ET523 and T. asperellum ET537 with PIRG of 87.1% and 88.9%, respectively. Meanwhile, T. reesei ET501 showed the strongest antibiosis activity with 100% inhibition in 80% concentration, compared to T. asperellum ET523 and T. asperellum ET537 which gave 12.3% and 90.5% of inhibition, respectively.

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Cultural and Morphological Characterization of Trichoderma spp. Associated with Green Mold Disease of Pleurotus spp. in Kashmir

Cited by 40 publications

References 1 publication

Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma

Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma

Effect of Trichoderma sp. on Anthracnose Disease of Stored Chilli

THE POTENTIAL OF ENDOPHYTIC Trichoderma FROM OIL PALM (Elaeis guineensis Jacq.) ROOTS OF NORTH SUMATRA, INDONESIA AGAINST Ganoderma boninense

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