Cultural conditions for the production of bacteriocin by a native isolate of Lactobacillus delbruecki ssp. bulgaricus CFR 2028 in milk medium

Balasubramanyam, B. V.; Varadaraj, M. C.

doi:10.1046/j.1365-2672.1997.00326.x

Cited by 22 publications

(11 citation statements)

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“…Samples were collected at regular intervals to measure the cell growth of the strain (A 600 ), pH change and bacteriocin activity (AU/ml). The influence of growth conditions such as incubation time, medium pH and incubation temperature on the production of bacteriocin was also carried out by the method described by Balasubramanyam & Varadaraj (1998) and Yildirim & Johnson (1998).…”

Section: Effect Of Medium Components and Growth Conditions On Bacterimentioning

confidence: 99%

Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Prema¹,

Bharathy²,

Palavesam³

et al. 2006

World J Microbiol Biotechnol

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Section: Effect Of Medium Components and Growth Conditions On Bacterimentioning

confidence: 99%

Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Prema¹,

Bharathy²,

Palavesam³

et al. 2006

World J Microbiol Biotechnol

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“…and Bifidobacterium sp. [18,20-22]. The use of these organisms offers many advantages, such as bacteriocins [14,17,19,22].…”

Section: Introductionmentioning

confidence: 99%

Effects of the probiotic Lactobacillus animalis in murine Mycobacterium avium subspecies paratuberculosis infection

et al. 2013

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BackgroundMAP is a suspected zoonotic pathogen and the causative agent of Johne’s Disease in cattle and other ruminant animals. With over $1 billion dollars in loss to the dairy industry due to Johne’s Disease, efforts to eliminate or reduce MAP from cattle are of importance. The purpose of this study was to determine if daily intake of probiotics could eliminate or reduce Johne’s Disease associated symptoms and pathogenesis by MAP. Post infection, animals are often asymptomatic carriers with limited shedding of the pathogen, proving early detection to be difficult. Disease and symptoms often appear 3–4 years after infection with antibiotic treatment proving ineffective. Symptoms include chronic gastrointestinal inflammation leading to severe weight-loss from poor feed and water intake cause a wasting disease. These symptoms are similar to those found in individuals with Crohn’s Disease (CD); MAP has been implicated by not proven to be the causative agent of CD. Probiotics administered to livestock animals, including dairy and beef cattle have demonstrated improvements in cattle performance and health. Our objectives included determining the benefits of Lactobacillus animalis (strain name: NP-51) in MAP infected BALB/c mice by evaluating systemic and gastrointestinal response by the host and gut microbiota. Male and female animals were fed 1×106 CFU/g probiotics in sterile, powdered mouse chow daily and infected with 1 × 107 CFU/ml MAP and compared to controls. Animals were evaluated for 180 days to assess acute and chronic stages of disease, with sample collection from animals every 45 days. MAP concentrations from liver and intestinal tissues were examined using real time-PCR methods and the expression of key inflammatory markers were measured during MAP infection (interferon-gamma [IFN-Υ], Interleukin-1α, IL-12, IL-10, IL-6, and Tumor necrosis factor alpha [TNF-α]).ResultsOur results demonstrate administration of probiotics reduces production of IFN-Υ and IL-6 while increasing TNF-α and IL-17 in chronic disease; healthful immune responses that reduce chronic inflammation associated to MAP infection.ConclusionsWe observed that the immune system’s response in the presence of probiotics to MAP contributes towards host health by influencing the activity of the immune system and gut microbial populations.

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“…The predominant species in this study L. delbrueckii subsp. bulgaricus and L. amylovorus, may produce the bacteriocin as described in some strains according to the previous reports (25,26). However, the relationship between probiotic lactobacilli and intestinal indigenous lactobacilli is very complex; we must consider not only bacterial ecology but also physiological responses of the intestine, such as absorption and secretion, digesta kinetics and retention time.…”

Section: Discussionmentioning

confidence: 97%

Stimulation of Indigenous Lactobacilli by Fermented Milk Prepared with Probiotic Bacterium, Lactobacillus delbrueckii subsp. bulgaricus Strain 2038, in the Pigs

Ohashi

Tokunaga

Taketomo

et al. 2007

J Nutr Sci Vitaminol

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SummaryThe aim of this study was to evaluate the effect of feeding yoghurt, prepared with Lactobacillus delbrueckii subsp . bulgaricus strain 2038, on indigenous lactobacilli in the pig cecum. Three female pigs fistulated at the cecum were fed 250 g of this yoghurt that contained over 10 11 colony-forming units of L. delbrueckii subsp . bulgaricus strain 2038 with their daily meal for 2 wk. The relative abundance and the composition of cecal lactobacilli was monitored by analysis of bacterial 16S rDNA with real time PCR and amplified bacterial rDNA restriction analysis using Lactobacillus -group specific primers, respectively, for 2 wk prior to, at the end of 2 wk of and 2 wk after the administration of this yoghurt. The relative abundance of lactobacilli was significantly increased by feeding yoghurt ( p Ͻ 0.01), although the bacterial 16S rDNA matching L. delbrueckii subsp . bulgaricus strain 2038 was not detected by amplified bacterial rDNA restriction analysis during this study. The number of operational taxonomic units (OTUs) detected was increased with feeding of the yoghurt in all pigs. At the same time, the estimated cell number of each OTU was increased with feeding of the yoghurt. It is demonstrated that continuous consumption of the probiotic lactobacilli will stimulate the growth of some indigenous lactobacilli and alter the composition of the lactobacilli.

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Cultural conditions for the production of bacteriocin by a native isolate of Lactobacillus delbruecki ssp. bulgaricus CFR 2028 in milk medium

Cited by 22 publications

References 0 publications

Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Effects of the probiotic Lactobacillus animalis in murine Mycobacterium avium subspecies paratuberculosis infection

Stimulation of Indigenous Lactobacilli by Fermented Milk Prepared with Probiotic Bacterium, Lactobacillus delbrueckii subsp. bulgaricus Strain 2038, in the Pigs

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