Culture of airway epithelial cells collected by a nasal brushing technique

Bridges, Michael A.

doi:10.1007/s11626-997-0026-1

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…As advocated by the Cardiff team, we used sterile cytology brushes which are soft and probably easier for subjects to tolerate [21]. Several authors perform nasal brushing under direct visualization of the inferior nasal turbinate [27,29,30,34,38]. However, as the Lisboa group [21], we did not use rhinoscopy which we felt more aggressive to the infant than simply introducing the brush into the nostril.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants

Mosler

Coraux

Fragaki

et al. 2008

Journal of Cystic Fibrosis

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Section: Discussionmentioning

confidence: 99%

Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants

Mosler

Coraux

Fragaki

et al. 2008

Journal of Cystic Fibrosis

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“…As far as T cells are concerned, our understanding of their activity in the nasal mucosa is based on extrapolation, using cell cultures as a starting point, that can be obtained from brushed nasal epithelial cells (23,24). There are also studies on biopsies from nasal polyps showing the role of cytokines, such as tumor necrosis factor-α (TNF-α) (25) or other cytokines (26).…”

Section: Introductionmentioning

confidence: 99%

Differences in the distribution of CD20, CD3, CD34 and CD45RO in nasal mucosa and polyps from patients with chronic rhinosinusitis

et al. 2019

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The present study focused on the assessment of the inflammatory infiltrate that characterizes nasal polyps in patients with chronic rhinosinusitis and nasal polyposis. Inflammatory cell type was determined using specific markers. This evaluation was made possible by determining the expression of the following markers: CD20, a marker of B lymphocytes [using activated T cells (ATC) armed with CD20 antibody]; CD3, a marker of T lymphocytes (using ATC armed with anti-CD3 antibody); CD45, the leukocyte common antigen (using ATC armed with anti-CD45 antibody; and CD34, for the microvasculature of the nasal polyp (using anti-CD34 antibody). The diagnosis of chronic rhinosinusitis with nasal polyps (CRSwNP) was made according to current EPOS guidelines based on patient history, clinical examination and nasal endoscopy. We examined surgically resected nasal polyps from 127 patients diagnosed with CRSwNP, who benefited from surgical procedures at the Department of Otorhinolaryngology of our institution. The polyps were analyzed at the Department of Pathology of our institution utilizing histopathological and immunohistochemical methods as follows: Firstly, the tissues were paraffin-impregnated, sectioned and stained with hematoxylin and eosin. We then examined the expression of CD3, CD20, CD34 and CD45RO by immunohistochemistry with soluble labeled streptavidin biotin (LSAB)/horseradish peroxidase (HRP) complexes. We observed the following histopathological changes: The structure of the epithelium was evidenced by collagenous subjacent stroma with mixed areas, sometimes associated with hyaline zones. In all types of polyps, we also observed a diffuse underlayer or periglandular lymphoplasmacytic in filtrate composed predominantly from T lymphocytes and eosinophils. The histopathological changes suggest the chronic inflammation of the sinus mucosa, which was diffusely distributed in allergic polyps and with nodular distribution in fibro-inflammatory polyps. The number of B lymphocytes was greater in the fibro-inflammatory polyps. On the whole, the findings of this study indicate that the inflammatory infiltrate in nasal polyps from patients with CRSwNP is mainly composed of T cells and eosinophils in all types of polyposis. In addition, a diffuse distribution of allergic polyps and the nodular distribution of fibro-inflammatory polyps, and the hyperplasia of the seromucous glands was observed. The determination of CD20, CD3, CD34 and CD45RO could be used to assess the inflammatory infiltrate of the nasal poplyps in these patients.

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Polarized Cultures of Human Airway Epithelium From Nasal Scrapings and Bronchial Brushings

Lopez-Souza

Avila

Widdicombe

2003

In Vitro Cell Dev Biol Anim

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Airway epithelial cultures are generally derived from tracheas postmortem or from surgical specimens of nasal polyps or turbinates. Scrapings of the mucosal surface have been little used as starting material for cultures because of their low yield of epithelial cells and their contamination with mucous secretions, blood, and underlying connective tissue. For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport.

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Culture of airway epithelial cells collected by a nasal brushing technique

Cited by 3 publications

References 4 publications

Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants

Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants

Differences in the distribution of CD20, CD3, CD34 and CD45RO in nasal mucosa and polyps from patients with chronic rhinosinusitis

Polarized Cultures of Human Airway Epithelium From Nasal Scrapings and Bronchial Brushings

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