Culture of Whole Mouse Embryos at Early Postimplantation to Organogenesis Stages

Rivera-Pérez, Jaime A.; Jones, Vanessa; Tam, Patrick

doi:10.1016/s0076-6879(10)76011-0

Cited by 39 publications

(22 citation statements)

References 14 publications

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“…Each of these analyses focused on a single factor or provided limited data for multiple stages of gastrulation. Comparisons of factor expression were further obscured by the combination of the speed of progression through gastrulation (Downs and Davies, 1993;Rivera-Pérez et al, 2010), differences in embryo staging, and different types of expression analyses among the studies. In addition, cell-based experiments demonstrated that relatively small changes in the levels of Oct4, Sox2 and Nanog have a significant influence on the specification of lineages during differentiation in vitro (Chambers et al, 2003;Mitsui et al, 2003;Niwa et al, 2000;Thomson et al, 2011).…”

Section: Dynamic Expression Of Pluripotency Factors During Gastrulationmentioning

confidence: 99%

“…Therefore, examining the onset of mesoderm specification in Tcf7l1 −/− embryos presented the possibility of identifying a novel role in coordinating cell fates with morphogenesis. However, the rapid formation of the PS during gastrulation generates substantial variability in developmental stage between individual embryos in a single litter (Downs and Davies, 1993;Rivera-Pérez et al, 2010), and distinct markers have not been identified in the mouse to separate the specification of mesoderm from the induction of morphological PS formation. In chickens, inhibition of the Wnt planar cell polarity (PCP) pathway blocked cell movement in the PS without inhibiting mesoderm gene expression (Voiculescu et al, 2007).…”

Section: Coupling Of Mesoderm Specification and Primitive Streak Indumentioning

confidence: 99%

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Tcf7l1 prepares epiblast cells in the gastrulating mouse embryo for lineage specification

Hoffman

Merrill

2013

Development

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SUMMARYThe core gene regulatory network (GRN) in embryonic stem cells (ESCs) integrates activities of the pro-self-renewal factors Oct4 (Pou5f1), Sox2 and Nanog with that of an inhibitor of self-renewal, Tcf7l1 (Tcf3). The inhibitor function of Tcf7l1 causes dependence on extracellular Wnt/β-catenin signaling activity, making its embryonic role within the ESC GRN unclear. By analyzing intact mouse embryos, we demonstrate that the function of Tcf7l1 is necessary for specification of cell lineages to occur concomitantly with the elaboration of a three-dimensional body plan during gastrulation. In Tcf7l1 −/− embryos, specification of mesoderm is delayed, effectively uncoupling it from the induction of the primitive streak. Tcf7l1 repressor activity is necessary for a rapid switch in the response of pluripotent cells to Wnt/β-catenin stimulation, from one of self-renewal to a mesoderm specification response. These results identify Tcf7l1 as a unique factor that is necessary in pluripotent cells to prepare them for lineage specification. We suggest that the role of Tcf7l1 in mammals is to inhibit the GRN to ensure the coordination of lineage specification with the dynamic cellular events occurring during gastrulation.

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Section: Dynamic Expression Of Pluripotency Factors During Gastrulationmentioning

confidence: 99%

Section: Coupling Of Mesoderm Specification and Primitive Streak Indumentioning

confidence: 99%

Tcf7l1 prepares epiblast cells in the gastrulating mouse embryo for lineage specification

Hoffman

Merrill

2013

Development

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“…Embryos were staged morphologically as described previously (Downs and Davies, 1993; Rivera-Perez et al, 2010) or were described in terms of dissection time. Noon of the day that a mating plug was observed was considered embryonic day 0.5 of development (E0.5).…”

Section: Methodsmentioning

confidence: 99%

Conditional Aurora A deficiency differentially affects early mouse embryo patterning

Yoon

Cowley

Gallant

et al. 2012

Developmental Biology

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Aurora A is a mitotic kinase involved in centrosome maturation, spindle assembly and chromosome segregation during the cell division cycle. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation of Aurora A in the epiblast or visceral endoderm layers of the conceptus leads to apoptosis and inhibition of embryo growth, causing lethality and resorption at approximately E9.5. The effects on embryo patterning, however, depend on the tissue affected by the mutation. Embryos with an epiblast ablation of Aurora A properly establish the anteroposterior axis but fail to progress through gastrulation. In contrast, mutation of Aurora A in the visceral endoderm, leads to posteriorization of the conceptus or failure to elongate the anteroposterior axis. Injection of ES cells into Aurora A epiblast knockout blastocysts reconstitutes embryonic development to E9.5, indicating that the extra-embryonic tissues in these mutant embryos can sustain development to organogenesis stages. Our results reveal new ways to induce apoptosis and to ablate cells in a tissue-specific manner in vivo. Moreover, they show that epiblast-ablated embryos can be used to test the potency of stem cells.

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“…Embryos were staged based on morphological landmarks as previously described (Downs and Davies, 1993; Rivera-Perez et al, 2010) or described in terms of dissection time. Noon of the day that a mating plug was observed was considered embryonic day 0.5 (E0.5) of gestation.…”

Section: Methodsmentioning

confidence: 99%

Extra-embryonic Wnt3 regulates the establishment of the primitive streak in mice

Yoon

Huang

Tortelote

et al. 2015

Developmental Biology

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The establishment of the head to tail axis at early stages of development is a fundamental aspect of vertebrate embryogenesis. In mice, experimental embryology, genetics and expression studies have suggested that the visceral endoderm, an extra-embryonic tissue, plays an important role in anteroposterior axial development. Here we show that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak. Embryos lacking Wnt3 in the visceral endoderm, however, appear normal by E9.5. Our results suggest a model for axial development in which multiple signals are required for anteroposterior axial development in mammals.

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Culture of Whole Mouse Embryos at Early Postimplantation to Organogenesis Stages

Cited by 39 publications

References 14 publications

Tcf7l1 prepares epiblast cells in the gastrulating mouse embryo for lineage specification

Tcf7l1 prepares epiblast cells in the gastrulating mouse embryo for lineage specification

Conditional Aurora A deficiency differentially affects early mouse embryo patterning

Extra-embryonic Wnt3 regulates the establishment of the primitive streak in mice

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