Culture of wool follicle dermal papilla cells from two breeds of sheep

Withers, Anne P.; Jahoda, Colin A.B.; Ryder, M. L.; Oliver, R. F.

doi:10.1007/bf00417536

Cited by 18 publications

(3 citation statements)

References 7 publications

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“…We initially acquired DPCs and DFs from goat skins, and exhibited they possess obviously heterogeneous external appearances. This inconformity was identical with human [68], mice [36] and other animals [69, 70]. Previous studies found that skin implantation of cultured DPCs induced new hair growth, whereas DFs can’t [36, 71].…”

Section: Discussionsupporting

confidence: 54%

Synchronous profiling and analysis of mRNAs and ncRNAs in the dermal papilla cells from cashmere goats

Wang

Zhou

et al. 2019

BMC Genomics

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Background Dermal papilla cells (DPCs), the “signaling center” of hair follicle (HF), delicately master continual growth of hair in mammals including cashmere, the fine fiber annually produced by secondary HF embedded in cashmere goat skins. Such unparalleled capacity bases on their exquisite character in instructing the cellular activity of hair-forming keratinocytes via secreting numerous molecular signals. Past studies suggested microRNA (miRNAs) and long non-coding RNAs (lncRNAs) play essential roles in a wide variety of biological process, including HF cycling. However, their roles and related molecular mechanisms in modulating DPCs secretory activities are still poorly understood. Results Here, we separately cultivated DPCs and their functionally and morphologically distinct dermal fibroblasts (DFs) from cashmere goat skins at anagen. With the advantage of high throughput RNA-seq, we synchronously identified 2540 lncRNAs and 536 miRNAs from two types of cellular samples at 4th passages. Compared with DFs, 1286 mRNAs, 18 lncRNAs, and 42 miRNAs were upregulated, while 1254 mRNAs, 53 lncRNAs and 44 miRNAs were downregulated in DPCs. Through overlapping with mice data, we ultimately defined 25 core signatures of DPCs, including HOXC8 and RSPO1 , two crucial activators for hair follicle stem cells (HFSCs). Subsequently, we emphatically investigated the impacts of miRNAs and lncRNAs ( cis - and trans - acting) on the genes, indicating that ncRNAs extensively exert negative and positive effects on their expressions. Furthermore, we screened lncRNAs acting as competing endogenous RNAs (ceRNAs) to sponge miRNAs and relief their repressive effects on targeted genes, and constructed related lncRNAs-miRNAs- HOXC8 / RSPO1 interactive lines using bioinformatic tools. As a result, XR_310320.3-chi-miR-144-5p- HOXC8 , XR_311077.2-novel_624- RSPO1 and others lines appeared, displaying that lncRNAs might serve as ceRNAs to indirectly adjust HFSCs status in hair growth. Conclusion The present study provides an unprecedented inventory of lncRNAs, miRNAs and mRNAs in goat DPCs and DFs. We also exhibit some miRNAs and lncRNAs potentially participate in the modulation of HFSCs activation via delicately adjusting core signatures of DPCs. Our report shines new light on the latent roles and underlying molecular mechanisms of ncRNAs on hair growth. Electronic supplementary material The online version of this article (10.1186/s12864-019-5861-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 54%

Synchronous profiling and analysis of mRNAs and ncRNAs in the dermal papilla cells from cashmere goats

Wang

Zhou

et al. 2019

BMC Genomics

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“…Dermal papilla cells have been cultured from a wide variety of species, including rat, 2 sheep 3 and red deer 4 . They are believed to be a useful model system for studying many aspects of hair growth 5 .…”

Section: Discussionmentioning

confidence: 99%

Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts

Bratka‐Robia¹,

Mitteregger²,

Aichinger³

et al. 2002

Veterinary Dermatology

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Skin biopsies were taken from female dogs, the primary hair follicles isolated and the dermal papilla dissected. After incubation in supplemented Amniomax complete C100 medium in 24-well culture plates, the dermal papilla cells (DPC) grew to confluence within 3 weeks. Thereafter, they were subcultivated every 7 days. Dermal fibroblast (DFB) cultures were established by explant culture of interfollicular dermis in serum-free medium, where they reached confluence in 10 days. They were subcultivated every 5 days. For immunohistochemistry, cells were grown on cover slips for 24 h, fixed and stained with antibodies against collagen IV and laminin. DPC showed an aggregative growth pattern and formation of pseudopapillae. Intensive staining for collagen IV and laminin could be observed until the sixth passage. DFB grew as branching, parallel lines and showed only weak staining for collagen IV and laminin.

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“…A more recent approach, which has exploited the relative ease with which hair follicles can be taken apart, is that of culturing cell populations from specific components-including the dermal papilla of rodents,"J hurnans,ll and sheep. 12 The biological relevance of vibrissa dermal papilla cell cultures was enhanced when a bioassay showed that, even after passaging, these cells were still able to elicit hair growth when reimplanted into inactivated f0llicles. ~3 The interactive roles played by the other major cell populations of the hair follicle remain relatively obscure.…”

Section: Introductionmentioning

confidence: 99%