Curcumin in dimethyl sulfoxide: Stability, spectral, luminescent and acid-base properties

Tsaplev, Yu. B.; Lapina, V. A.; Trofimov, Aleksei V.

doi:10.1016/j.dyepig.2020.108327

Cited by 22 publications

(26 citation statements)

References 26 publications

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“…Cancer cells express more AQPs in their cytoplasmic membrane than normal cells; thus, combination therapy of CAP and CUR has more effect on B16-F10 when compared to the L929 cell line. Previous studies reported the solvent can affect the physicochemical properties of CUR [66][67][68]. Therefore, DMSO and culture medium may have a role in the interactions between CUR and AQP channels.…”

Section: Discussionmentioning

confidence: 98%

Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Yazdani

Mehrabanjoubani

Rafiei

et al. 2021

BioMed Research International

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Curcumin (CUR) has interesting properties to cure cancer. Cold atmospheric plasma (CAP) is also an emerging biomedical technique that has great potential for cancer treatment. Therefore, the combined effect of CAP and CUR on inducing cytotoxicity and apoptosis of melanoma cancer cells might be promising. Here, we investigated the combined effects of CAP and CUR on cytotoxicity and apoptosis in B16-F10 melanoma cancer cells compared to L929 normal cells using MTT method, acridine orange/ethidium bromide fluorescence microscopic assay, and Annexin V/PI flow cytometry. In addition, the activation of apoptosis pathways was evaluated using BCL2, BAX, and Caspase-3 (CASP3) gene expression and ratio of BAX to BCL2 (BAX/BCL2). Finally, in silico study was performed to suggest the molecular mechanism of this combination therapy on melanoma cancer. Results showed that although combination therapy with CUR and CAP has cytotoxic and apoptotic effects on cancer cells, it did not improve apoptosis rate in melanoma B16-F10 cancer cells compared to monotherapy with CAP or CUR. In addition, evaluation of gene expression in cancer cell line confirmed that CUR and CAP concomitant treatment did not enhance the expression of apoptotic genes. In silico analysis of docked model suggested that CUR blocks aquaporin- (AQP-) 1 channel and prevents penetration of CAP-induced ROS into the cells. In conclusion, combination therapy with CAP and CUR does not improve the anticancer effect of each alone.

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Section: Discussionmentioning

confidence: 98%

Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Yazdani

Mehrabanjoubani

Rafiei

et al. 2021

BioMed Research International

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“…The main challenge with CUR is its instability at neutral or basic pH and also its poor absorption when taken orally or applied topically. [ 40 ] CUR exists in the forms of diketone and keto-enol and thereby has three acidic protons, two phenolic ones and one enolic proton[ 41 ], Fig. 1 .…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

et al. 2022

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Curcumin (CUR), a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, is used as a pharmaceutical agent, spice in food, and as a dye. Currently, CUR is being investigated for cancer treatment in Phase-II clinical trials. CUR also possesses excellent activities like anti-inflammatory, anti-microbial, and anti-oxidant, therefore quality control is crucial. The present research work was to develop a new, simple, validated and time-saving rapid 96-well plate spectrofluorimetric method for the determination of CUR. The developed method was compared with routinely used high performance liquid chromatography (HPLC) technique. The developed method were found to be linear in the concentration range of 15 to 3900 ng/mL with R2 ≥ 0.9983 for spectrofluorimetric and 50-7500 ng/mL with R2 ≥ 0.9999 for HPLC method. Accuracy, intraday and interday precision was adequate, with RSD lower than the suggested limits. The limits for the detection and the quantification of CUR were 7 and 15 ng/mL for spectrofluorimetric, and 25 and 50 ng/mL for HPLC respectively. The Bland-Altman analysis demonstrated the similarities between the two methods. The 96-well plate method was successfully applied to determine CUR in solid lipid nanoparticles (SLNs) and chitosan nanoparticles (Chi-NPs). The developed spectrofluorimetric method can hence serve as a possible replacement for the HPLC method for the quantification of CUR in healthcare and food products.

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“…The clinical use of curcumin is limited because of its low bioavailability, hydrophobic nature of the molecule, poor water solubility, low oral absorbability, and rapid metabolic rate. Concerning the persistence of curcumin towards oxidation, a recent study is noteworthy [ 17 ]. It has been found that curcumin is essentially inert towards autooxidation in aerated alkaline DMSO at room temperature.…”

Section: Introductionmentioning

confidence: 99%

“…It has been found that curcumin is essentially inert towards autooxidation in aerated alkaline DMSO at room temperature. This is highly surprising, since curcumin is prone to rapid autooxidation in basic aqueous solutions and, furthermore, this is astonishing because in alkaline DMSO the autooxidation of an appreciable number of substances proceeds particularly fast [ 17 ]. Besides this unprecedented behavior, in such a medium curcumin exhibits also rather unusual spectral and luminescent properties [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Natural Chain-Breaking Antioxidants and Their Synthetic Analogs as Modulators of Oxidative Stress

et al. 2021

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Oxidative stress is associated with the increased production of reactive oxygen species or with a significant decrease in the effectiveness of antioxidant enzymes and nonenzymatic defense. The penetration of oxygen and free radicals in the hydrophobic interior of biological membranes initiates radical disintegration of the hydrocarbon “tails” of the lipids. This process is known as “lipid peroxidation”, and the accumulation of the oxidation products as peroxides and the aldehydes and acids derived from them are often used as a measure of oxidative stress levels. In total, 40 phenolic antioxidants were selected for a comparative study and analysis of their chain-breaking antioxidant activity, and thus as modulators of oxidative stress. This included natural and natural-like ortho-methoxy and ortho-hydroxy phenols, nine of them newly synthesized. Applied experimental and theoretical methods (bulk lipid autoxidation, chemiluminescence, in silico methods such as density functional theory (DFT) and quantitative structure–activity relationship ((Q)SAR) modeling) were used to clarify their structure–activity relationship. Kinetics of non-inhibited and inhibited lipid oxidation in close connection with inhibitor transformation under oxidative stress is considered. Special attention has been paid to chemical reactions resulting in the initiation of free radicals, a key stage of oxidative stress. Effects of substituents in the side chains and in the phenolic ring of hydroxylated phenols and biphenols, and the concentration were discussed.

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Curcumin in dimethyl sulfoxide: Stability, spectral, luminescent and acid-base properties

Cited by 22 publications

References 26 publications

Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

Natural Chain-Breaking Antioxidants and Their Synthetic Analogs as Modulators of Oxidative Stress

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