Curcumin Loaded Fibrinogen Nanoparticles for Cancer Drug Delivery

Abstract

In this work we prepared and evaluated the curcumin loaded fibrinogen nanoparticles (CRC-FNPs) as a novel drug delivery system for cancer therapy. These novel CRC-FNPs were prepared by a two-step co-acervation method using calcium chloride as the cross-linker. The prepared nanoparticles were characterized using dynamic light scattering (DLS), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetry (TG), differential thermal analysis (DTA) and X-ray diffraction (XRD) studies. DLS studies showed that the particle size of CRC-FNPs was in the range of 150-200 nm. The loading efficiency (LE) and in vitro drug release were studied using UV spectrophotometer. The LE was found to be 90%. The cytotoxicity was studied using L929 (mouse fibroblast), PC3 (prostate) and MCF7 (breast) cancer cell lines by MTT assay, which confirmed that CRC-FNPs were comparatively non toxic to L929 cell line while toxic to PC3 and MCF7 cancer cells. Cellular uptake of CRC-FNPs studied using L929, MCF-7 and PC3 cells monitored by fluorescent microscopy, demonstrated significant internalization and retention of nanoparticles inside the cells. The preferential accumulation of curcumin within the cancer cells were also confirmed by flowcytometry based uptake studies. The apoptosis assay showed increased apoptosis on MCF-7 compared to L929 cells. The blood compatibility of CRC-FNPs throws light on the fact that it is possible to administer the prepared nanoformulation intravenously. The results indicated that CRC-FNPs could be a promising therapeutic agent for cancer treatment.

Biocompatible, biodegradable and thermo-sensitive chitosan-g-poly (N-isopropylacrylamide) nanocarrier for curcumin drug delivery

“…The amine peak at 1639 is shifted to 1637 cm −1 due to the interaction with TPP, and with curcumin loading the peak again shifted to 1635 cm −1 . The wavenumber shift from higher frequency region to lower frequency region could be attributed to the fact that as TTP crosslinking reaction proceeds, the bond length would be decreased/disturbed, which ultimately resulted in wavenumber shift as described above [27][28][29][30]. Apart from the amine peak shift, the amide shift was observed from 1560 to 1555 cm −1 which throws light on the possibility of amide interaction with the crosslinking agent.…”

Curcumin-loaded biocompatible thermoresponsive polymeric nanoparticles for cancer drug delivery

“…An exciting potential solution in cancer treatments is to encapsulate the drug in a biocompatible material that can be injected into the blood stream with the intention of delivering drug to a tumor site in response to an external thermal source like radio frequency (RF) generator, which is the source that provides radio waves to heat and kill cancer cells [2]. Chitosan is a well-known biopolymer having many applications in tissue engineering [3][4][5][6], wound healing [7], drug delivery [8][9][10][11], and also in gene delivery [12,13]. In recent years, much interest has been given on stimuli-sensitive polymeric systems that show a phase transition in response to external stimulus such as temperature, pH, specific ion, and electric field [14].…”

Micelles Based on Methoxy Poly(Ethylene Glycol)Cholesterol Conjugate for Controlled and Targeted Drug Delivery of a Poorly Water Soluble Drug

“…[14][15][16] Thereinto, amphiphilic copolymers micellar carriers with hydrophobic inner core has been widely investigated during the last two decades. [17][18] For pharmaceutical applications, biodegradable and biocompatible amphiphilic copolymers are usually used to prepare micelles with relatively high stability because of their low critical micelle concentration (CMC) values.…”