Current state and latest advances in the concept, production and functionality of prebiotic oligosaccharides

Moreno, F. Javier; Corzo, Nieves; Montilla, Antonia; Villamiel, Mar; Olano, Agustı́n

doi:10.1016/j.cofs.2017.02.009

Cited by 82 publications

(39 citation statements)

References 45 publications

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“…There have been two methods for lactosucrose production [8]. One is to transfer the β-galactosyl group produced by the decomposition of lactose to the C4 hydroxyl group of glucosyl in sucrose by the catalysis of β-galactosidase (EC 3.2.1.23); The other is to transfer the fructose group produced by the decomposition of sucrose to the C1 hydroxyl group at the reducing end of the lactose by the catalysis of βfructofuranosidase (EC 3.2.1.26) or levansucrase (EC 2.4.1.10).…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Chen

Deng

et al. 2020

Bioengineered

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As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six βfructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant βfructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable βfructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Chen

Deng

et al. 2020

Bioengineered

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“…One way to achieve this is by improving the nutritional quality of food and increase the spread of functional foods, nutraceuticals and dietary Correspondence: Veselin Bivolarski (morydin@abv.bg), Department of Biochemistry and Microbiology, Plovdiv University "Paisii Hilendarski", 24 Tzar Assen Str., 4000 Plovdiv, Bulgaria Abbreviations: DP, degree of polymerization; GH70, glycoside hydrolase family 70; GOSs, glucooligosaccharides; M/S, maltose / sucrose; L/S, lactose / sucrose; R/S, raffinose / sucrose; L., Leuconostoc; Lb., Lactobacillus; LAB, lactic acid bacteria; μ, specific growth rate; mMRS, modified medium of de Man, Rogosa and Sharpe supplements [1]. In this regard, the concept of prebiotics is gaining an ever wider realization in the practice of producing healthy foods [2,3]. On the basis of the scientific knowledge that has accumulated up to now, it is clear that oligosaccharides and polysaccharides of microbial origin have proven prebiotic properties, and are particularly desirable sweeteners in the low-calorie foods [4,5].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Bivolarski

Vasileva

Gabriel³

et al. 2018

Engineering in Life Sciences

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In the present work, we report an efficient synthesis of glucooligosaccharides (GOSs) with prebiotic potential by novel glucansucrase URE 13–300 from Leuconostoc mesenteroides URE 13 strain. The highest total yield of GOSs with degree of polymerization (DP) from 3 to 6 was obtained with maltose as an acceptor and maltose/sucrose (M/S) ratio 1–136 g/L. An efficient modulation of GOSs composition is achieved by varying the M/S ratio. At M/S = 1, 2, 4 and 7 the content of DP3 products gradually increase from 54.50 to 91.70%. When the M/S ratio was decreased the synthesis of DP>3 GOSs is predominant and reaches 75.60% (M/S = 0.25). In addition, the maltose derived GOSs with DP>3, as well as raffinose and lactose glucosylation products have a branched structure which is prerequisite for increased prebiotic potential. The synthesized GOSs were efficiently metabolized by probiotic strains of Lb. plantarum S26, Lb. brevis S27 and Lb. sakei S16, and the calculated values of specific growth rate (μ) were nearly identical to this on glucose media, when maltose derived GOSs were used as a carbohydrate source. Strain specific features were observed in the utilization of the synthesized GOSs, as well as in the production of lactic acid and acetic acid.

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“…is also a recognized prebiotic for the treatment of constipation and systemic portal encephalopathy (Corzo-Martínez et al, 2013). Given the huge interest in recent years towards the gastrointestinal function and new structures with improved properties, new routes to obtain a second-generation of prebiotic oligosaccharides are being explored (Moreno et al, 2017). This is the case of the oligosaccharides derived from lactulose (OsLu).…”

Section: Introductionmentioning

confidence: 99%

Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model

Ferreira-Lazarte

Montilla

Mulet-Cabero

et al. 2017

Journal of Functional Foods

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Current state and latest advances in the concept, production and functionality of prebiotic oligosaccharides

Cited by 82 publications

References 45 publications

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model

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