Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model

Ferreira-Lazarte, Álvaro; Montilla, Antonia; Mulet-Cabero, Ana-Isabel; Rigby, Neil M.; Olano, Agustı́n; Mackie, Alan; Villamiel, Mar

doi:10.1016/j.jff.2017.03.031

Cited by 24 publications

(21 citation statements)

References 21 publications

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“…However, there are some in vivo studies performed with neonatal and growing rats showing that GOS are selectively digested in the small intestine 7,8 , challenging the assumption that GOS reach the colon with their original structure fully intact. Similar findings have been recently reported with in vitro digestion models using a rat small intestinal extract 9,10 .…”

Section: Introductionsupporting

confidence: 91%

Trans-β-galactosidase activity of pig enzymes embedded in the small intestinal brush border membrane vesicles

Julio-González

Hernández‐Hernández

Moreno

et al. 2019

Sci Rep

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This work highlights the utility of brush border membrane vesicles (BBMV) from the pig small intestine as a reliable model for gathering information about the reaction mechanisms involved in the human digestion of dietary carbohydrates. Concretely, the elucidation of the transgalactosylation mechanism of pig BBMV to synthesize prebiotic galacto-oligosaccharides (GOS) is provided, unravelling the catalytic activity of mammalian small intestinal β-galactosidase towards the hydrolysis of GOS. This study reveals that pig BBMV preferably synthesizes GOS linked by β-(1 → 3) bonds, since major tri- and disaccharide were produced by the transfer of a galactose unit to the C-3 of the non-reducing moiety of lactose and to the C-3 of glucose, respectively. Therefore, these results point out that dietary GOS having β-(1 → 3) as predominant glycosidic linkages could be more prone to hydrolysis by mammalian intestinal digestive enzymes as compared to those linked by β-(1 → 2), β-(1 → 4), β-(1 ↔ 1) or β-(1 → 6). Given that these data are the first evidence on the transglycosylation activity of mammalian small intestinal glycosidases, findings contained in this work could be crucial for future studies investigating the structure-small intestinal digestibility relationship of a great variety of available prebiotics, as well as for designing tailored fully non-digestible GOS.

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Section: Introductionsupporting

confidence: 91%

Trans-β-galactosidase activity of pig enzymes embedded in the small intestinal brush border membrane vesicles

Julio-González

Hernández‐Hernández

Moreno

et al. 2019

Sci Rep

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“…Similar behaviour was obtained in a previous work comparing the digestibility of prebiotics added to milk in an in vitro study with a rat extract. 20…”

Section: Tables 2 and 3 Show The Quantitative Determination Of Indivimentioning

confidence: 99%

“…17,18,19 Recently, the use of mammalian intestinal enzymes has been reported as an excellent alternative method to determine carbohydrate digestion. 20,21,22 In vivo and in vitro studies have reported considerable digestion rates in the small intestine of different types of GOS in rats 20,23,14,24,25 questioning the general acceptance that these compounds reach intact the colon. These authors also have reported a different resistance to the upper gastrointestinal tract conditions as well as a different effect on microbiota depending on the main β-linkage in the mixture.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation

Ferreira-Lazarte

Gallego-Lobillo

Moreno

et al. 2019

J. Agric. Food Chem.

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Small intestinal brush border membrane vesicles (BBMV) from pig were used to evaluate the digestibility of different mixtures of galactooligosaccharides from lactose (GOS), differing in the predominant glycosidic linkage, and from lactulose (OsLu). Dissimilar hydrolysis rates were detected after BBMV digestion. Predominant glycosidic linkages and monomeric composition showed to play a key role in the resistance to intestinal mammalian digestive enzymes. β(1→3) GOS mixture was the most susceptible to hydrolysis with 50.2 % of degradation after digestion, followed by β(1→4) with 34.9 % hydrolysis, whereas β(1→6) linkages showed to be highly resistant to digestion (27.1 %). Monomeric composition seems to provide a better resistance in β(1→6) oligosaccharides from lactulose (22.8 %) as compared to β(1→6)-GOS (27.1 %). This was also observed in β-galactosyl-fructoses and β-galactosyl-glucoses disaccharides where the presence of fructose provided a higher resistance to digestion. Thus, the resistance to small intestinal digestive enzymes highly depends on structural characteristic and composition of prebiotic ingredients. Increasing knowledge on this regard could contribute to the future synthesis of new tailored prebiotic with specific functional properties.

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“…12,13 Recently, a promising in vitro digestibility method of dietary carbohydrates using rat small intestinal extract (RSIE) has questioned the belief that recognized prebiotics oligosaccharides derived from lactose (GOS) and fructooligosaccharides (FOS) reach the distal portions of colon without alterations. [14][15][16] Therefore, with the aim of gain more insight on the benefits of non-digestible carbohydrates, in this work the digestibility of commercial functional fiber, such as α-galactooligosaccharides derived from peas and different types of fructans have been tested using a rat small intestine extract.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

et al. 2020

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Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model

Abstract: This is a repository copy of Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model.

Cited by 24 publications

References 21 publications

Trans-β-galactosidase activity of pig enzymes embedded in the small intestinal brush border membrane vesicles

Trans-β-galactosidase activity of pig enzymes embedded in the small intestinal brush border membrane vesicles

In Vitro Digestibility of Galactooligosaccharides: Effect of the Structural Features on Their Intestinal Degradation

Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers

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