Cyanidin-3-glucoside prevents hydrogen peroxide (H2O2)-induced oxidative damage in HepG2 cells

Tan, Jiaqi; Li, Pengcheng; Xue, Hongkun; Li, Qian

doi:10.1007/s10529-020-02982-2

Cited by 23 publications

(22 citation statements)

References 39 publications

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“…As a key proinflammatory cytokine, TNF‐a might participate in the lymphocyte‐mediated adaptive immune response by initiating apoptosis (Li et al., 2021 ). C3G may inhibit apoptosis by suppressing the activation of cleaved caspase‐9/3 or p53 related protein and regulating the expression of apoptosis genes in hydrogen peroxide‐induced HepG2 cells (Tan et al., 2020 ). Phenolic compounds carvacrol could reduce LPS‐induced inflammation by inhibiting the transcriptional activation of NF‐κB in macrophages, and exhibited antiapoptotic activity (Somensi et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

Hao

Guan

Huang

et al. 2021

Food Science & Nutrition

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Inflammation is a response of the body to stimulation, which may be beneficial or harmful. Inflammation may either be acute or chronic, and is related to the homeostasis of physiological systems in vivo. Inflammation is associated with many diseases and is the basis of most pathological processes. The main causes of inflammation are infection and tissue damage, which can lead to the concentration of leukocytes and plasma proteins (Medzhitov, 2008).Chronic inflammation is involved in many diseases, including diabetes, obesity, atherosclerosis, and cancer (Coussens & Werb, 2002;

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Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

Hao

Guan

Huang

et al. 2021

Food Science & Nutrition

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“…The production and clearance of ROS in normal cells are in a dynamic equilibrium. The oxidative injury environment can lead to an overexpression of intracellular ROS, 54 followed by excessive ROS accumulation, inducing severe cell injuries, such as apoptosis, necrosis, and DNA damage. 55,56 The intracellular ROS production was measured to evaluate the cellular oxidative stress generated by H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

“…58,63,64 Furthermore, ROS and lipid peroxidation are the most typical indicators of oxidative damage. 54 An essential step in cell membrane degradation is the ROS reaction with the double bonds of polyunsaturated fatty acids to yield lipid hydroperoxides. 53 SSeCAHK may inhibit lipid peroxidation by reducing ROS production, decreasing the MDA concentration.…”

Section: Mda Levelsmentioning

confidence: 99%

Antioxidant activity of SSeCAHK in HepG2 cells: a selenopeptide identified from selenium-enriched soybean protein hydrolysates

Zhang

et al. 2021

RSC Adv.

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“…Due to the excellent antioxidant and anti‐inflammatory activities, C3G has been found to play a pivotal role in various chronic oxidative/inflammation‐related diseases, including colitis (Gan et al, 2020), arthritis (Sun & Li, 2018), atherosclerosis (Wang et al, 2020), neurodegenerative disorders (Sukprasansap et al, 2020), allergic asthma (Ma et al, 2019), bacterial keratitis (Li et al, 2020), diabetic complications (Zheng et al, 2020), alcoholic/nonalcoholic fatty liver disease (Tan et al, 2020; Zhou et al, 2020), and cancer (Baster et al, 2020). IDD has been found to be associated with chronic oxidative stress and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Cyanidin‐3‐glucoside protects against high glucose‐induced injury in human nucleus pulposus cells by regulating the Nrf2/HO‐1 signaling

Bai

Lian

et al. 2022

J of Applied Toxicology

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Cyanidin-3-glucoside (C3G) is a well-known natural anthocyanin with antioxidant and anti-inflammatory properties. In this study, we explored the role and action mechanism of C3G in high glucose (HG)-induced damage of human nucleus pulposus cells (HNPCs). Cell viability was assessed by CCK-8 assay. TUNEL assay was performed for detecting apoptotic rate. Western blot was performed to determine the expression levels of cl-caspase-3, caspase-3, Bax, Bim, collagen II, aggrecan, MMP-3, MMP-13, and ADAMTS5. Reactive oxygen species (ROS) generation was analyzed using DCFH-DA staining. The Nrf2 was knocked down or overexpressed in HNPCs through transfection with si-Nrf2 or pcDNA3.0-Nrf2. C3G treatment (12.5, 25, and 50 μM) improved cell viability of HNPCs under HG condition. HG-induced cell apoptosis of HNPCs was attenuated by C3G with decreased apoptotic rate and relative levels of cl-caspase-3/caspase-3, Bax, and Bim. C3G treatment caused significant increase in expression levels of collagen II and aggrecan and decrease in the relative levels of MMP-3, MMP-13, and ADAMTS5. After treatment with C3G, ROS generation in HNPCs was markedly reduced. Treatment with N-acetylcysteine (NAC) reversed HG-induced cell apoptosis and extracellular matrix (ECM) degradation. C3G treatment induced the expression of Nrf2 and HO-1 in HG-induced HNPCs. Moreover, knockdown of Nrf2 reversed the inhibitory effect of C3G on ROS production. Summarily, C3G exerted a protective effect on ROS-mediated cellular damage in HNPCs under HG condition, which was attributed to the induction of the Nrf2/ HO-1 signaling pathway. K E Y W O R D S cyanidin-3-glucoside (C3G), high glucose, human nucleus pulposus cells (HNPCs), intervertebral disc degeneration (IDD), Nrf2/HO-1 pathway 1 | INTRODUCTION Intervertebral disc (IVD) degeneration (IDD) is a natural process coming with aging. However, aberrant degeneration of IVD in young population is a pathological event associated with low back pain. IDD is characterized by progressive structural failure in IVD that alters disc height and spine mechanics, which leads to chronic segmental spinal instability (Stergar et al., 2019). Thus, IDD affects the patient's life quality and causes economic and social burden. Conservative and invasive approaches are commonly used for the treatment of IDD in

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Cyanidin-3-glucoside prevents hydrogen peroxide (H2O2)-induced oxidative damage in HepG2 cells

Cited by 23 publications

References 39 publications

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

Antioxidant activity of SSeCAHK in HepG2 cells: a selenopeptide identified from selenium-enriched soybean protein hydrolysates

Cyanidin‐3‐glucoside protects against high glucose‐induced injury in human nucleus pulposus cells by regulating the Nrf2/HO‐1 signaling

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