Cyanobacterial Bioreporters as Sensors of Nutrient Availability

Bullerjahn, George S.; Boyanapalli, Ramakrishna; Rozmarynowycz, Mark J.; McKay, R. Michael L.

doi:10.1007/10_2009_23

Cited by 9 publications

(7 citation statements)

References 122 publications

(194 reference statements)

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“…Thus, these complex biological interactions with Fe chemistry challenge the quantification of Fe bioavailability in marine environments. In this context, whole-cell Fe-dependent cyanobacterial bioreporter represent a promising tool to determine Fe bioavailability and map the extent and severity of Fe limitation in marine systems These genetically modified organisms produce a bioluminescent signal proportional to the concentration of bioavailable Fe in the cell environment (Bullerjahn et al, 2010). Up to date, the sole cyanobacterial Fe bioreporter available for marine systems was constructed by transformation of the cyanobacterium Synechococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Acclimation to Iron Limitation Reveals New Insights in Metabolism Regulation of Synechococcus sp. PCC7002

2017

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In large areas of the ocean phytoplankton growth is limited by the scarcity of iron (Fe), an essential co-factor for multiple enzymes. Phytoplankton has hence developed strategies to survive under Fe limitation. Here, we characterize the response to Fe limitation of the cyanobacterium Synechococcus sp. PCC7002 acclimated to different Fe concentrations in chemically characterized synthetic seawater. The inorganic Fe concentrations used represent levels of Fe limitation relevant for different domains of the contemporary ocean. Combining physiological and transcriptomic approaches, we provide evidence of the progression of the physiological responses to increasing levels of Fe limitation. Our results showed a rising number of significantly regulated genes and the complexity of the response to increasing Fe limitation. Mild Fe limitation induced up-regulation of genes involved in Fe uptake, while genes involved in photosynthesis and respiration were down-regulated. Strong Fe limitation induced up-regulation of genes involved in energy metabolism and concomitant down-regulation of macronutrients uptake. Severe Fe limitation affected fine metabolic regulation of co-factors expression and activation of anti-oxidative stress responses. Our results suggest that homeostasis under long-term Fe limitation put at play dramatically different mechanisms for oxidative stress mitigation and carbon metabolism than those previously reported under Fe stress. Hence, evidence the importance of acclimation processes on the performance of cyanobacteria under Fe limitation conditions.

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Section: Introductionmentioning

confidence: 99%

Long-Term Acclimation to Iron Limitation Reveals New Insights in Metabolism Regulation of Synechococcus sp. PCC7002

2017

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“…Fe-responsive bioreporters are normally constructed using ecologically relevant cyanobacteria species belonging to the Synechococcus and Synechocystis genus (Bullerjahn et al, 2010). These bioreporters usually carry a transcriptional fusion of the Vibrio harveyi luxAB genes and the isiAB promoter responsive to iron deficiency.…”

Section: Bacterial Bioreporter Applications In Environmental Assesmentioning

confidence: 99%

“…Synechococcus sp. strains PCC 7942 and PCC 7002 have been engineered to carry such reporter constructs, and the resulting bioreporters, designated as KAS101 (Durham et al, 2002) and CCMP2669 (Boyanapalli et al, 2007; Bullerjahn et al, 2010), respectively, have been used to assess Fe bioavailability in the Great Lakes (Hassler et al, 2009; McKay et al, 2005; Porta et al, 2005) and marine environments (Boyanapalli et al, 2007). These bioreporters function such that the bioluminescent response increases with reducing concentration of intracellular Fe 3+ , making them suitable for low concentration detection.…”

Section: Bacterial Bioreporter Applications In Environmental Assesmentioning

confidence: 99%

Genetically modified whole-cell bioreporters for environmental assessment

Sayler

et al. 2013

Ecological Indicators

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Living whole-cell bioreporters serve as environmental biosentinels that survey their ecosystems for harmful pollutants and chemical toxicants, and in the process act as human and other higher animal proxies to pre-alert for unfavorable, damaging, or toxic conditions. Endowed with bioluminescent, fluorescent, or colorimetric signaling elements, bioreporters can provide a fast, easily measured link to chemical contaminant presence, bioavailability, and toxicity relative to a living system. Though well tested in the confines of the laboratory, real-world applications of bioreporters are limited. In this review, we will consider bioreporter technologies that have evolved from the laboratory towards true environmental applications, and discuss their merits as well as crucial advancements that still require adoption for more widespread utilization. Although the vast majority of environmental monitoring strategies rely upon bioreporters constructed from bacteria, we will also examine environmental biosensing through the use of less conventional eukaryotic-based bioreporters, whose chemical signaling capacity facilitates a more human-relevant link to toxicity and health-related consequences.

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“…Whole-cell bioreporters are cells genetically engineered to produce a dose-dependent measurable signal in response to chemical or physical agents in their environment (Harms et al, 2006;van der Meer and Belkin, 2010). Due to their ecological relevance, low cost and easy maintenance, cyanobacteria have been used for whole-cell bioreporter development which has demonstrated to be useful to assess toxicity in photosynthetic organisms (Shao et al, 2002;Rodea-Palomares et al, 2009, 2010, nutrient bioavailability in fresh waters (Schreiter et al, 2001;Bullerjahn et al, 2010;Munoz-Martin et al, 2011, 2014a, 2014b as well as specific families of pollutants like heavy metals (Erbe et al, 1996;Peca et al, 2008;Martin-Betancor et al, 2015). At present, there exist whole-cell bioreporters for oxidative stress detection mostly based on recombinant heterotrophic bacteria such as E. coli (Belkin et al, 1996;Gu et al, 2004;Nizai et al, 2007Nizai et al, , 2008Ivask et al, 2010;Woutersen et al, 2011;Ooi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion

et al. 2018

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This work describes the construction of two novel self-luminescent bioreporter strains of the cyanobacterium Nostoc sp. PCC 7120 by fusing the promoter region of the sodA and sodB genes (encoding the superoxide dismutases MnSod and FeSod, respectively) to luxCDABE from Photorhabdus luminescens aimed at detecting pollutants that generate reactive oxygen species (ROS), particularly O. Bioreporters were tested against methyl viologen (MV) as the inducer of superoxide anion (O). Both bioreporters were specific for O and Limits of detection (LODs) and Maximum Permissive Concentrations (MPCs) were calculated: Nostoc sp. PCC 7120 pBG2154 (sodA) had a range of detection from 400 to 1000 pM of MV and for Nostoc sp. PCC 7120 pBG2165 (sodB) the range of detection was from 500 to 1800 pM of MV after 5 h-exposure. To further validate the bioreporters, they were tested with the emerging pollutant Triclosan which induced bioluminescence in both strains. Furthermore, the bioreporters performance was tested in two real environmental samples with different water matrix complexity, spiked with MV. Both bioreporters were induced by O in these environmental samples. In the case of the river water sample, the amount of bioavailable MV as calculated from the bioreporters output was similar to that nominally added. For the waste water sample, the bioavailable MV concentration detected by the bioreporters was one order of magnitude lower than nominal. These differences could be due to MV complexation with organic matter and/or co-occurring organic contaminants. These results confirm their high sensitivity to O and their suitability to detect oxidative stress-generating pollutants in fresh-waters.

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Cyanobacterial Bioreporters as Sensors of Nutrient Availability

Cited by 9 publications

References 122 publications

Long-Term Acclimation to Iron Limitation Reveals New Insights in Metabolism Regulation of Synechococcus sp. PCC7002

Long-Term Acclimation to Iron Limitation Reveals New Insights in Metabolism Regulation of Synechococcus sp. PCC7002

Genetically modified whole-cell bioreporters for environmental assessment

Two novel cyanobacterial bioluminescent whole-cell bioreporters based on superoxide dismutases MnSod and FeSod to detect superoxide anion

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