This study evaluated the effect of air-particle abrasion protocols on the biaxial flexural strength, surface characteristics and phase transformation of zirconia after cyclic loading. Disc-shaped zirconia specimens (Ø: 15mm, thickness: 1.2mm) (N=32) were submitted to one of the air-particle abrasion protocols (n=8 per group): (a) 50 m Al2O3 particles, (b) 110 m Al2O3 particles coated with silica (Rocatec Plus), (c) 30 m Al2O3 particles coated with silica (CoJet Sand) for 20s at 2.8bar pressure. Control group received no air-abrasion. All specimens were initially cyclic loaded (×20,000, 50N, 1Hz) in water at 37°C and then subjected to biaxial flexural strength testing where the conditioned surface was under tension. Zirconia surfaces were characterized and roughness was measured with 3D surface profilometer. Phase transformation from tetragonal to monoclinic was determined by Raman spectroscopy. The relative amount of transformed monoclinic zirconia (FM) and transformed zone depth (TZD) were measured using XRD. The data (MPa) were analyzed using ANOVA, Tukey's tests and Weibull modulus (m) were calculated for each group (95% CI). The biaxial flexural strength (MPa) of CoJet treated group (1266.3±158(A)) was not significantly different than that of Rocatec Plus group (1179±216.4(A,B)) but was significantly higher than the other groups (Control: 942.3±74.6(C); 50 m Al2O3: 915.2±185.7(B,C)). Weibull modulus was higher for control (m=13.79) than those of other groups (m=4.95, m=5.64, m=9.13 for group a, b and c, respectively). Surface roughness (Ra) was the highest with 50 m Al2O3 (0.261 m) than those of other groups (0.15-0.195 m). After all air-abrasion protocols, FM increased (15.02%-19.25%) compared to control group (11.12%). TZD also showed increase after air-abrasion protocols (0.83-1.07 m) compared to control group (0.59 m). Air-abrasion protocols increased the roughness and monoclinic phase but in turn abrasion with 30 m Al2O3 particles coated with silica has increased the biaxial flexural strength of the tested zirconia. 942.3±74.6 C ; 50 µm Al 2 O 3 : 915.2±185.7 B,C ). Weibull modulus was higher for control (m=13.79) than those of other groups (m=4. 95, m=5.64, m=9.13 for group a, b and c, respectively). Surface roughness (R a ) was the highest with 50 µm Al 2 O 3 (0.261 µm) than those of other groups (0.15-0.195 µm). After all air-abrasion protocols, F M increased (15.02-19.25%) compared to control group (11.12%). TZD also showed increase after air-abrasion protocols (0.83-1.07 µm) compared to control group (0.59 µm). Air-abrasion protocols increased the roughness and monoclinic phase but in turn abrasion with 30 µm Al 2 O 3 particles coated with silica has increased the biaxial flexural strength of the tested zirconia.