Cyclocephalogenin and cyclogalegigenin from Astragalus caucasicus

Cycloascauloside b from Astragalus caucasicus

“…This was confirmed by C-5 of the aglycon appearing at 54.56 ppm as an isolated resonance that did not experience a β-effect of glycosylation at C-6. The same can be said about the C-7 resonance at 38.58 ppm [6][7][8]. Table 1 shows that C-3 of the aglycon had the greatest chemical shift (88.79 ppm) whereas those of C-6, C-16, and C-24 were unchanged.…”

“…The isolation and separation of triterpenoids from A. caucasicus have been reported [1,2]. PMR and 13 C NMR spectra in C 5 D 5 N were obtained on Bruker AM-400 and DRX-500 instruments with TMS internal standard.…”

“…The CHCl 3 extract was washed with water and evaporated. The solid was chromatographed over a column of silica gel with elution by CHCl 3 :CH 3 OH (15:1) to isolate 2 (30 mg), mp 132-133°C (CH 3 OH), which was identified by direct comparison with an authentic sample on TLC [8]. The aqueous part of the hydrolysate was neutralized, condensed, and chromatographed on paper using C 5 D 5 N:C 6 H 6 :BuOH:H 2 O (3:5:1:3).…”