Cycloheximide-induced phenolic burst in roots of Pisum sativum L.

Tarchevsky, I. A.; Ageeva, Marina; Petrova, N. V.; Akulov, A. N.; Egorova, A. M.

doi:10.1134/s0003683817050155

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…41) The increase of enzymes involved in defense metabolism and cell wall formation is similarly accelerated by cycloheximide in Pisum sativum. 42) In Arabidopsis thaliana, it has been shown that the production of cell walls also promotes the defensive response and slows plant development. 43) The combination of those factors may clarify the processes by which cycloheximide inhibits seed germination in O. minor, while it is still important to confirm if similar effects are present in Orobanche.…”

Section: Discussionmentioning

confidence: 99%

Cycloheximide in the nanomolar range inhibits seed germination of <i>Orobanche minor</i>

Nogami,

Nagata,

Imada

et al. 2024

J. Pestic. Sci.

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From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of Streptomyces sp. no. 226 inhibited Orobanche minor seed germination without significantly affecting the seed germination of Trifolium pratense and the growth of Aspergillus oryzae and Escherichia coli . Using ESI-MS, 1 H-NMR, and 13 C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of O. minor and 2.5 µg/mL for that of the conidial germination of A . oryzae . Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of O. minor plays a critical role in the inhibition of O. minor seed germination. Our data suggested that O. minor RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing S. cerevisiae RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders O. minor seed germination.

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Section: Discussionmentioning

confidence: 99%