In recent years, commercial glyphosate herbicide formulations have been widely used in agriculture to control aquatic and terrestrial weeds. These pesticides may result in disruption of ecological balance, causing damage to nontarget organisms, including fish. Carassius auratus L. specimenes were exposed to glyphosate herbicide in concentration of 2.5mg/L and, respectively, 5 mg/L for 120 h, in laboratory condition. Thus, the effects of this chemical agent on both hematological parameters (hemoglobin, hematocrit, number of red blood cells) and on oxidative status indices (SOD, CAT, GPX, MDA) at Prussian carp experimental lots were analyzed. Our results showed, in comparison with the reference batch, the influence of glyphosate on the analyzed hematological and biochemical parameters, in close correlation with the administered pesticide dose.