Cystic fibrosis and control nasal epithelial cells harvested by a brushing procedure

Bridges, Michael A.; Walker, David C.; Davidson, A. G. F.

doi:10.1007/bf02633211

Cited by 30 publications

(24 citation statements)

References 10 publications

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“…1C). With results similar to ours, other authors described the percentages of epithelial cells in nasal brushings of non-CF control subjects and patients with CF to be approximately 70% to 87% (Bridges et al, 1991), but this decreased to 65% in patients with chronic rhinitis (Chapelin et al, 1996). With regard to specific cell types in nasal brush samples, our results revealed a similar distribution among non-CF subjects, carriers, and patients with CF, ie, nonsignificant differences in all comparisons of specific cell types among the three groups analyzed (see Fig.…”

Section: Characterization Of Cell Population In Nasal Brushing Samplessupporting

confidence: 87%

“…This is mainly because of the invasiveness and risk of most techniques aimed at collecting human cells (eg, biopsies), the small number of cells thus collected, or the limited number, poor quality, and nonrepresentative nature of samples resulting from surgery (such as nasal polypectomies or lung transplants). Brushing of the respiratory tract, however, a noninvasive method originally proposed for ciliary studies (Kelsen et al, 1992;Rutland and Cole, 1980;Rutland et al, 1982), allows the easy sampling of numerous, representative, wellpreserved, and dissociated cells from the superficial mucosa (Bridges et al, 1991;Chapelin et al, 1996;Danel et al, 1996). The present study was thus performed on cell samples obtained by nasal brushings of F508del homozygous patients with CF, F508del carriers, and non-CF control subjects.…”

Section: Discussionmentioning

confidence: 99%

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Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Penque

Mendes

Beck

et al. 2000

Laboratory Investigation

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SUMMARY:Present state of knowledge, mostly based on heterologous expression studies, indicates that the cystic fibrosis transmembrane conductance regulator (CFTR) protein bearing the F508del mutation is misprocessed and mislocalized in the cytoplasm, unable to reach the cell surface. Recently, however, it was described that protein levels and localization are similar between F508del and wild-type CFTR in airway and intestinal tissues, but not in the sweat glands. In this study, we used immunocytochemistry with three different anti-CFTR antibodies to investigate endogenous CFTR expression and localization in nasal epithelial cells from F508del homozygous patients, F508del carriers, and non-CF individuals. On average, 300 cells were observed per individual. No significant differences were observed for cell type distributions among CF, carrier, and non-CF samples; epithelial cells made up approximately 80% to 95% of all cells present. CFTR was detected mostly in the apical region (AR) of the tall columnar epithelial (TCE) cells, ciliated or nonciliated. By confocal microscopy analysis, we show that the CFTR apical region-staining does not overlap with either anti-calnexin (endoplasmic reticulum), anti-p58 (Golgi), or anti-tubulin (cilia) stainings. The median from results with three antibodies indicate that the apical localization of CFTR happens in 22% of TCE cells from F508del homozygous patients with CF (n ϭ 12), in 42% of cells from F508del carriers (n ϭ 20), and in 56% of cells from healthy individuals (n ϭ 12). Statistical analysis indicates that differences are significant among all groups studied and for the three antibodies (p Ͻ 0.05). These results confirm the presence of CFTR in the apical region of airway cells from F508del homozygous patients; however, they also reveal that the number of cells in which this occurs is significantly lower than in F508del carriers and much lower than in healthy individuals. These findings may have an impact on the design of novel pharmacological strategies aimed at circumventing the CF defect caused by the F508del mutation. (Lab Invest 2000, 80:857-868).

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Section: Characterization Of Cell Population In Nasal Brushing Samplessupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Penque

Mendes

Beck

et al. 2000

Laboratory Investigation

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“…The nasal brushing and culture technique has been introduced for cystic fibrosis patients instead of bronchial lavage and bronchial brushing. 8 That technique is less invasive to patients, less expensive, and easier to perform than others. Also, intranasal brushing and direct cytology sampling are used to confirm the pathology of upper airway diseases and provide the ability to analyze the functional properties of airway cells.…”

Section: Alternative Methods For Primary Nasal Epithelial Cell Culturementioning

confidence: 99%

Alternative Method for Primary Nasal Epithelial Cell Culture Using Intranasal Brushing and Feasibility for the Study of Epithelial Functions in Allergic Rhinitis

Park

Kim

et al. 2016

Allergy Asthma Immunol Res

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Purpose: Although differentiated normal human nasal epithelial (NHNE) cells can be used to study the role of human nasal epithelium, there is a need for effective culture models of nasal epithelium in sinonasal disease status, including allergic rhinitis (AR). We aimed to examine the feasibility of intranasal brushing for culture of nasal epithelial cells in AR patients and to verify the hypothesis that allergic nasal epithelial (ARNE) cells differ in histologic and physiologic characteristics. Methods: We established a system for isolating (via intranasal brushing) and culturing (with air-liquid interface, ALI) nasal epithelial cells from healthy volunteers (n=8) and AR patients (n=8). We used this system to compare the histologic findings and physiologic characteristics of NHNE and ARNE. Results: The histology results showed that fully differentiated ALI culture was obtained at least 14 days after confluence and that both ciliated and secretory cells were well differentiated in ALI culture using nasal brushing. The histology results of ARNE culture were significantly different from NHNE. The number of ciliated cells was lower, and secretory cells were more dominant in ARNE cell culture compared to NHNE cells. We also observed, by electron microscopy, loose tight junctions and short cilia in cultured ARNE cells. In addition, the mRNA level of TSLP which was one of the epithelial-derived allergic cytokines was significantly higher, and the expressions of genes involved in ciliogenesis were lower in cultured ARNE cells without allergen stimulation. Conclusions: Our findings suggest that ALI culture of ARNE cells using intranasal brushing may be an alternative method for epithelial cell culture in AR patients and that cultured ARNE cells will be useful for in vitro studies of the mechanisms at play during AR because they maintain unique allergic characteristics.

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“…Hull and Harris, it should be stated that some problems they encountered might have been avoided if my original method had been published in extensio, instead of in the truncated Letter to the Editor format (1). Although my method was completely accurate as published, it would appear that it was not recounted in sufficient detail to permit successful repetition, despite these workers' obvious skill in the art of tissue culture.…”

mentioning

confidence: 94%

“…Hull and Harris (5) regarding their experience using my novel brushed human nasal epithelial (BHNE) cell culture method (1). Examination of their published manuscript (5) reveals that Drs.…”

mentioning

confidence: 97%

Culture of airway epithelial cells collected by a nasal brushing technique

Bridges

1997

In Vitro Cell.Dev.Biol.-Animal

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Cystic fibrosis and control nasal epithelial cells harvested by a brushing procedure

Cited by 30 publications

References 10 publications

Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Alternative Method for Primary Nasal Epithelial Cell Culture Using Intranasal Brushing and Feasibility for the Study of Epithelial Functions in Allergic Rhinitis

Culture of airway epithelial cells collected by a nasal brushing technique

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