Cytochemical and Histochemical Aspects of the Digestive Gland Cells of the Mussel Mytilus galloprovincialis (L.) in Relation to Function

Abstract

The epithelium of the digestive tubules of the mussel Mytilus galloprovincialis is comprised of two cell types, namely digestive and basophilic cells. In basophilic cells, the secretory granules are beta-glucuronidase immunoreactive, a fact that enhances the hypothesis that beta-glucuronidase is synthesized in basophilic cells. A novel observation at the ultrastructural level is the pinocytic activity associated with the formation of coated pits. This observation constitutes direct evidence for endocytic processes taking place in basophilic cells. The use of cryostat sections from the same digestive tubules reveals, in many instances, a very pronounced neutral lipid accumulation in the same structures giving a positive reaction for N-acetyl-beta-hexosaminidase, indicating the association of those lipids with lysosomes. In some mussels, a high content of lipofuscin was observed in the lysosomes of the digestive cells. In these cases, the lysosomal structures show a limited neutral lipid content, and a weaker N-acetyl-beta-hexosaminidase reaction. In the digestive cells, the carbohydrate content of the lysosomes, and very well-developed canal system in the apical part of cells are discussed in relation to their function.

Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

“…Overall, the results further support the hypothesis that the lysosomal system represents a significant target for estrogenic compounds in molluscan cells (Moore et al, 1978;Canesi et al, 2004aCanesi et al, ,b, 2006Canesi et al, , 2007 like in mammalian cells (Szego et al, 1975). In bivalves, the hepatopancreas plays a central role in metabolism through intracellular digestion of food particles and in distribution of nutrients to reproductive tissues during gamete maturation (Dimitriadis et al, 2004). Therefore, the overall effects induced by BPA in this tissue may result in indirect effects on reproduction.…”

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

“…그러나 NL 의 세포 내 과잉 축적은 세포의 사멸(cell death) 혹은 세 (Moore 1988;Krishnakumar et al 1994;Kagley et al 2003;Dimitriadis et al 2004). 또 한 항구 주변이나 공업단지 주변에 서식하는 담치의 소화 맹낭에도 LF 축적이 높은 것을 세포화학적인 방법을 통해 많은 연구에서 보고된 바 있다 (Lowe 1988;Moore 1988;Viarengo and Nott 1993;Krishnakumar et al 1994;Kagley et al 2003;Moore et al 2004).…”

“…그러나 조직관찰을 위한 파라 핀 블록 제작은 그 준비 기간이 오래 걸리며, 고정과 포매 과정에서 지질성분 혹은 효소의 활성을 잃어버리는 단점 이 있다 (Fink 1986 (Fink 1986;Lowe 1988;Moore 1988;Krishnakumar et al 1994;Dimitriadis et al 2004). 이와 같은 이유로, 대부분의 해산 이매패류 소화맹 낭 리소솜 활성의 연구는 동결절편 된 조직에서 세포화학 적인 방법을 이용하여 이루어지고 있다 (Lowe 1988;Moore 1988;Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003;Dimitriadis et al 2004). 또한 동결절편은 세포막의 지질성분을 파괴하지 않기 때문에 전자현미경 및 면역조직화학기법 등 다양한 각도에서 연 구가 가능하다 (Dimitriadis et al 2004).…”

“…이와 같은 이유로, 대부분의 해산 이매패류 소화맹 낭 리소솜 활성의 연구는 동결절편 된 조직에서 세포화학 적인 방법을 이용하여 이루어지고 있다 (Lowe 1988;Moore 1988;Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003;Dimitriadis et al 2004). 또한 동결절편은 세포막의 지질성분을 파괴하지 않기 때문에 전자현미경 및 면역조직화학기법 등 다양한 각도에서 연 구가 가능하다 (Dimitriadis et al 2004).…”

Ultrastructure of the Digestive Diverticulum of Saxidomus purpuratus (Bivalvia: Veneridae)

“…(Owen, 1972;Morton, 1983;Henry et al, 1991;Eble, 2001;Dimitriadis et al, 2004;Robledo et al, 2006). 류들 (Owen, 1972;Morton, 1983;Henry et al, 1991;Eble, 2001;Dimitriadis et al, 2004;Robledo et al, 2006 …”