Cytochemical examination of blood cells of children with chronic tonsillitis and type 1 diabetes mellitus

Gavrylenko, Iurii; Laiko, Andrey; Karas, A.F.; Karas, Hunter

doi:10.15587/2519-4798.2018.127424

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…,Q SDWLHQWV VX˜HULQJ IURP 7'0 WKH FDUGLRvascular system undergoes the greatest changes, its disorders leading to involvement of the vascular, nervous and other systems, in particular the immune system, as well as to disability and mortality, one of the reasons for which is the accumulation of the end products of glycosylation [5].…”

Section: 'Lvfxvvlrqmentioning

confidence: 99%

Morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus

Havrylenko¹,

Laiko²,

Syniachenko³

et al. 2023

MPU

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Introduction. Increased susceptibility to infections in patients with type 1 diabetes mellitus is associated with impaired body protective functions as a result of suppressed immunity, increased cellular adhesion of microorganisms, susceptibility to catabolic processes. Purpose - to determine the morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus (EDM); to investigate the effect of the extract of seven medicinal plants BNO 1030 in them. Materials and methods. Experimental studies were conducted on 20 male Wistar rats in order to determine their level of nicotineamideadeninedinucleotide (NAD), reactive oxygen species in white blood cells and superoxide dismutase. All animals were separated: the Group 1 was the control group of healthy rats, the Group 2 - the control group receiving BNO 1030 extract, the Group 3 included animals with the EDM without treatment, the Group 4 - rats with the EDM, which received the BNO 1030 extract. Results. After 6 weeks of development of the EDM, the NAD blood level in diabetic rats was reduced and reached (0.270±0.017) µmol/L, which is lower than that in the control group ((0.357±0.021) µmol/L). The group of diabetic rats treated with BNO 1030 extract showed its increase to (0.341±0.012) μmol/L compared to the group of diabetic animals, which did not receive the drug. While assessing the viability of white blood cells, it has been established that the death of most of these cells occurs by apoptosis against the background of the EDM. When BNO 1030 was used in the group of diabetic rats, the level of apoptotic cells decreased and amounted to 13.7% compared to the group, which was not administered with the drug (20.8%). In addition, the level of apoptosis in control rats, which also received BNO 1030, decreased by 3% versus the control group. Conclusions. The reduction of NAD content in tissues and blood in experimental animals with the EDM was determined, and its increase by 24% following the administration of BNO 1030 was established, which proves positive effect of the drug on the course of energy processes and glycolysis - the main route of carbohydrate metabolism. When carrying out experiments with laboratory animals, all bioethical norms and recommendations were observed. No conflict of interests was declared by the authors.

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Section: 'Lvfxvvlrqmentioning

confidence: 99%

Morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus

Havrylenko¹,

Laiko²,

Syniachenko³

et al. 2023

MPU

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“…The course and outcomes of the disease, complete recovery in bacterial pathology, including streptococcal tonsillitis, are largely due to the timely and adequate response of specific and nonspecific protective factors, which include the state of cellular and humoral immune response and phagocytic activity of neutrophils (FAN) [9].…”

Section: Introductionmentioning

confidence: 99%

Immune response and phagocytosis of children with streptococcal tonsillitis and infected with the herpes virus type 6.

2021

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The aim of the work is to study the indicators of cellular and humoral immune response and FAN of patients with tonsillitis in children infected with HHV-6 type. Materials and methods of research. In 72 children aged 3–15 years (mean age 8.42±1.44 years), patients with streptococcal tonsillitis of moderate severity, the levels of the main subpopulations of blood lymphocytes were determined using monoclonal antibodies by solid-phase enzyme-linked immunosorbent assay, phagocytic activity of neutrophils (FAN) using the nitrosine tetrazolium reduction test (HCT test) and serum IgA, IgM, IgG levels by radial immunodiffusion. The studies were carried out in the acute period and in the period of convalescence. Mathematical and statistical processing of the data obtained was carried out using Microsoft Excel 2003 and Statistica 6.0 programs. The significance of differences between the mean values was determined using the Student's test (t). Results. Different mechanisms of formation of pathological process at streptococcal tonsillitis at children depending on the concomitant HHV-6 type infection are revealed. In patients with mixed infection in the acute period of tonsillitis determine the signs of deficiency of the cellular immune system with the maximum deviation of CD-lymphocytes (t=3.13 vs. t=2.16 in mono-infection), the tension in the humoral link of the immune response Ig deviation (t=1.63 against t=0.91 in mono-infection) on the background of phagocytosis violation in terms of FAN deviation (t=3.22 against t=2.01 in mono-infection). By the period of convalescence, the restoration of the imbalance of specific and non-specific protection factors does not occur. Increased IgG content in the acute period is one of the features of streptococcal tonsillitis in children on the background of HHV-6t infection. Conclusions. Concomitant VGL-6 type infection in children with streptococcal tonsillitis affects the mechanisms of formation of the inflammatory process and the reactivity of patients, forming the preconditions for adverse outcomes of the disease. The obtained results can be the basis for early detection of herpes virus infection in patients with tonsillitis, will help to optimize the management of patients in different periods of the disease.

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Cytochemical Status of Wistar Hannover Rats Peripheral Blood Leukocytes After the Influence of Modern Triazole Fungicides

Usenko¹

2018

VPBM

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Cytochemical examination of blood cells of children with chronic tonsillitis and type 1 diabetes mellitus

Cited by 4 publications

References 10 publications

Morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus

Morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus

Immune response and phagocytosis of children with streptococcal tonsillitis and infected with the herpes virus type 6.

Cytochemical Status of Wistar Hannover Rats Peripheral Blood Leukocytes After the Influence of Modern Triazole Fungicides

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