Cytochrome c covalently immobilized on mesoporous silicas as a peroxidase: Orientation effect

Kao, Kun‐Che; Lee, Chia‐Hung; Lin, Tien‐Sung; Mou, Chung‐Yuan

doi:10.1039/b925331a

Cited by 43 publications

(40 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the active site or its opening are oriented towards the pore wall, the substrate accessibility will be limited; hence, a lower enzyme activity. This was also shown through surface modifications of a mesoporous material that in the model could be used to selectively control the orientation of a peroxidase [47]. The surface had been modified with three different linkers binding to different sites on the enzyme, which caused different orientations of the enzyme upon binding to the linker.…”

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 97%

“…In relation to enzyme immobilization this allows protein modeling of the enzyme structure and calculation of surface potential or charge distribution on the surface at different pH (see Figure 9) [9,47,108,113]. This information can partly be used to predict how strongly enzymes can bind to the surface and how the strength depends on the environmental conditions upon immobilization [9].…”

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 99%

“…One property often denoted immobilization efficiency is the ratio of the amount of immobilized enzyme compared to the original amount of enzyme in the soaking solution [47][48][49][50]. This…”

Section: Adsorption Isotherms and The Rate Of Immobilizationmentioning

confidence: 99%

“…Additionally, enzyme orientation has been shown to be of great importance for the enzyme activity [47]. The accessibility of substrates to the active site is greatly influenced by the enzyme orientation.…”

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 99%

“…Iron containing heme groups can alter spin state upon adsorption onto silanol covered surfaces. This was studied using electron paramagnetic resonance (EPR) where vibration energies will vary between high and low spin state which was related to conformational changes and activation of cytochrome c [47].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

See 4 more Smart Citations

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Carlsson

Gustafsson

Thörn

et al. 2014

Advances in Colloid and Interface Science

222

167

View full text Add to dashboard Cite

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 97%

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 99%

Section: Adsorption Isotherms and The Rate Of Immobilizationmentioning

confidence: 99%

Section: Protein Modeling and Molecular Dynamics Simulationsmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

See 3 more Smart Citations

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Carlsson

Gustafsson

Thörn

et al. 2014

Advances in Colloid and Interface Science

222

167

View full text Add to dashboard Cite

Dynamic Covalent Bond‐Assisted Programmed and Traceless Protein Release: High Loading Nanogel for Systemic and Cytosolic Delivery

Wang

et al. 2018

Adv Funct Materials

View full text Add to dashboard Cite

Systemic and cytosolic delivery represents a grand challenge preventing many therapeutic proteins from clinical applications. Despite tremendous progresses in the past decade, most approaches generally lack the ability of triggered traceless protein release, require complicated formulation, and/or yield low protein loading. By using the protein as a crosslinker, here, a simple and general formulation affording protein nanogels (NG) with uniformed sizes and exceptionally high protein loading (>50%) is reported. By using the fine-tuned bis-and monosubstituted maleamic anhydride-amine chemistry for the crosslinking of the protein and a 4-armed PEG-MA 4 , the NG is implemented with a tandem pH-programmed and traceless release character. The final NG, CDM-MA-NG, is stable under normal physiological conditions and effectively protects the crosslinked cargo cytochrome C from serum fouling, proteolytic and thermal degradation. In vitro, CDM-MA-NG exhibits a high level of cellular uptake and potent cancer cell killing only when incubated at pH 6.5, but not 7.4. Systemic administration of CDM-MA-NG leads to significantly inhibited tumor growth and extended survival rate. Given the abundance of the amine groups on protein surface, this work describes a universal platform for therapeutic protein formulation, and opens up enormous opportunities for the systemic, cytosolic, and traceless delivery of proteinbased nanomedicines.

show abstract

Functional Silica Nanoparticles for Redox‐Triggered Drug/ssDNA Co‐delivery

Nguyen

Borah

et al. 2012

Adv Healthcare Materials

View full text Add to dashboard Cite

A mesoporous silica nanoparticle (MSNP) based co-delivery system is developed in order to deliver simultaneously drug and single strand DNA (ssDNA) in a controlled manner. Negatively charged ssDNA as a model gene is immobilized onto the surface of positively charged ammonium-functionalized MSNPs through electrostatic interaction, effectively blocking the loaded drugs within the mesopores of MSNPs. When the pre-installed disulfide bond on the ammonium unit is broken by the addition of the reducing agent such as dithiothreitol or glutathione, the ssDNA network on the surface is freed, leading to the release of the loaded drug molecules from the mesopores. The cell investigations indicate that the functional nanoparticles have a very low cytotoxicity under the concentrations measured. The doxorubicin-loaded and ssDNA-coated nanoparticles show an enhanced cellular internalization, leading to a successful drug/ssDNA co-delivery in vitro for significant apoptosis of Hela cancer cells as compared with that of free doxorubicin. The obtained experimental results indicate promising applications of the functional nanoparticles in cancer treatment.

show abstract

Cytochrome c covalently immobilized on mesoporous silicas as a peroxidase: Orientation effect

Cited by 43 publications

References 32 publications

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Dynamic Covalent Bond‐Assisted Programmed and Traceless Protein Release: High Loading Nanogel for Systemic and Cytosolic Delivery

Functional Silica Nanoparticles for Redox‐Triggered Drug/ssDNA Co‐delivery

Contact Info

Product

Resources

About