Cytochrome P450 1A mRNA in the guppy Phalloceros caudimaculatus and response to beta-naphthoflavone and environmental samples

Ferreira, Roger Stacke; Chivittz, Cíntia da Cruz; Santos, Guilherme Senna dos; Zanette, Juliano

doi:10.1016/j.aquatox.2016.10.023

Cited by 8 publications

(2 citation statements)

References 36 publications

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“…Similar to other cyprinodontiform fishes, e.g., Poecilia reticulata and Fundulus heteroclitus, it presents characteristics that favor its use as a model in environmental toxicology studies (Zanette, 2013). The P. caudimaculatus fish has been suggested as a model to evaluate the effects of pollution in the aquatic environment (Araújo et al, 2009;Chivittz et al, 2016;Ferreira et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Nolorbe-Payahua

Freitas

Roesch

et al. 2020

Heliyon

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Intestinal microbiota perform important functions for the health of fishes. Knowing the microbial composition and evaluating the possible effects caused by anthropogenic pollution in the intestinal microbiota of fish populations might represent an important step in defining microbial biomarkers for water pollution. This study evaluated the impact of environmental contamination on the gut microbiota of the livebearer killifish Phalloceros caudimaculatus. The 16S survey using the V4 region of the 16S rRNA gene was used to characterize and compare the microbiota of two P. caudimaculatus populations from streams with different levels of environmental contamination in Rio Grande, RS, Brazil. Twelve bacterial operational taxonomic units (OTUs) (around one-third of the total) were shared between both fish populations. They represent the core microbiota of the gut in this species. The dominant phyla were Protebacteria and Firmicutes, with more than 80% of relative abundance. The dominant genus was Burkholderia with more than 35% of the relative abundance irrespective of the environmental condition. We detected a lower microbial diversity (Shannon index and observed OTUs) in fish from the polluted stream compared to the reference stream. The PERMANOVA analysis showed that the intestinal microbial communities from fish living in the polluted stream were distinct from those found in the reference stream (p < 0.05). Finally, we identified Luteolibacter, Methylocaldum and Rhodobacter genera, which correlated strongly with the polluted stream. These taxa might represent potential microbial biomarkers of exposure to environmental contaminants in the guts of fish. Confirmation of these findings in other polluted environments might allow the development of a microbiota-based screening approach for environmental evaluation in ecotoxicological studies in aquatic ecosystems.

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Section: Introductionmentioning

confidence: 99%

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Nolorbe-Payahua

Freitas

Roesch

et al. 2020

Heliyon

Self Cite

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“…CYP1 is responsible for PAH biotransformation through aryl hydrocarbon receptormediated pathway in vertebrates (Chaty et al 2004;Jørgensen et al 2008;Won et al 2013). Many studies in shes have shown that the transcriptional level of CYP1A signi cantly increased in response to lipophilic xenobiotics (Uno et al 2012;Kim et al 2013a;Ferreira et al 2016). By contrast, CYP1A has not been observed in invertebrates.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs

Zhao

Wang

Yan

et al. 2022

Environ Sci Pollut Res

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Polychaete worms can biotransform polycyclic aromatic hydrocarbons (PAHs) in environments, and the cytochrome P450 (CYP) enzyme plays an important role in this process. Herein, a novel cytochrome P450 gene was identi ed and characterized from the polychaete worm Perinereis aibuhitensis. The full-length cDNA, which is named CYP4V82, is 1709 bp encoding a protein of 509 amino acids and has high similarity to CYP4V. The expression levels of CYP4V82 and CYP4BB4 (a CYP gene identi ed from P. aibuhitensis in a previous study, Chen et al., 2012) exposure to various concentrations of benzo[α]pyrene (B[α]P) (0.5, 2, 4, and 8 μg/L) and same mass concentrations of uoranthene (Flu, 3.2 μg/L), phenanthrene (Phe, 2.9 μg/L), B[α]P (4.0 μg/L) were detected to identify the function of the CYP4 family in P. aibuhitensis. Compared with CYP4BB4, CYP4V82 mRNA was minimally expressed on day 7 but highly sensitive on day 14. Notably, the expression level of CYP4V82 and CYP4BB4 was relatively different in response to PAHs with different benzene rings of the same concentration. The expression of CYP4V82 in the B(a)P group was the highest, while that of CYP4BB4 in the Phe group was relatively higher than the two other groups. These findings suggest that PAHs are associated with the induction of CYP4V82 and CYP4BB4 expressions, which may have different e ciencies in the detoxification of PAHs.

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Cytochrome P450 1A mRNA in the Gambusia affinis and Response to Several PAHs

et al. 2020

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Cytochrome P450 1A mRNA in the guppy Phalloceros caudimaculatus and response to beta-naphthoflavone and environmental samples

Cited by 8 publications

References 36 publications

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Environmental contamination alters the intestinal microbial community of the livebearer killifish Phalloceros caudimaculatus

Identification of a novel CYP4V gene in the polychaete Perinereis aibuhitensis: transcriptional comparison with a CYP4B gene exposed to PAHs

Cytochrome P450 1A mRNA in the Gambusia affinis and Response to Several PAHs

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