Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis

Tian, Lixing; Tang, Xin; Zhu, Junmin; Luo, Li; Ma, Xiaoyuan; Cheng, Siyao; Zhang, Wei; Tang, Wanqi; Ma, Wei; Yang, Xue; Lv, Chuanzhu; Liang, Huaping

doi:10.1186/s12964-020-0523-3

Cited by 34 publications

(29 citation statements)

References 56 publications

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“…These findings suggest that repeated administration of N-ZnO is more likely to affect immune response than B-ZnO. It was reported that CYP1A1 was highly expressed in inflammatory macrophages and the mice skin, inducing release of pro-inflammatory mediators and immune cell activation [50,51]. Hence, signaling pathway involving inflammation response can be a target for further mechanistic toxicity study.…”

Section: Discussionmentioning

confidence: 92%

Particle Size and Biological Fate of ZnO Do Not Cause Acute Toxicity, but Affect Toxicokinetics and Gene Expression Profiles in the Rat Livers after Oral Administration

Jin

Choi

2021

IJMS

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Zinc oxide (ZnO) particles have been used as dietary supplements because zinc is an essential trace element for humans. Along with the rapid development of nanotechnology, the use of ZnO nanoparticles (NPs) is increasing in the food industry, but their oral toxicity potential still remains to be answered. In this study, the effects of particle size and biological fate of ZnO on acute toxicity, toxicokinetics, and gene expression profiles in the livers were investigated after oral administration of ZnO NPs (N-ZnO), bulk-sized ZnO (B-ZnO) or Zn ions in rats. The plasma concentration-time profiles after a single-dose oral administration of ZnOs differed depending on particle/ionic forms and particle size, showing high absorption of Zn ions, followed by N-ZnO and B-ZnO, although in vivo solubility did not differ from particle size. No significant acute toxicity was found after oral administration of ZnOs for 14 days in rats. However, transcriptomic responses in the livers were differently affected, showing that metabolic process and metal biding were up-regulated by Zn ions and N-ZnO, respectively, which were not pronounced in the liver treated with B-ZnO. These findings will be useful to predict the potential oral toxicity of ZnO NPs and further mechanistic and long-term exposure studies are required to assume their safety.

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Section: Discussionmentioning

confidence: 92%

Particle Size and Biological Fate of ZnO Do Not Cause Acute Toxicity, but Affect Toxicokinetics and Gene Expression Profiles in the Rat Livers after Oral Administration

Jin

Choi

2021

IJMS

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“…It has been reported that genes of the CYP450 family varied similarly in the liver of experimental mice infected with E. multilocularis [ 30 ]. Moreover, a recent study demonstrated that members of the CYP family CYP1A could enhance inflammatory responses [ 48 ]. This aligns with the results of our previous study showing several upregulated miRNAs, which are related to NF-KB inflammation pathways, and play important roles in the response of intestinal tissue to E. granulosus infection in sheep with resistant haplotypes [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Intestinal transcriptomes in Kazakh sheep with different haplotypes after experimentalEchinococcus granulosusinfection

Song

Wang

et al. 2021

Parasite

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Cystic echinococcosis (CE) is a chronic zoonosis caused by infection with the larval stage of the cestode Echinococcus granulosus. As the intermediate host, sheep are highly susceptible to this disease. Our previous studies have shown that sheep with haplotype MHC Mva Ibc-Sac IIab-Hin1I ab were resistant to CE infection, while their counterparts without this haplotype were not. In order to reveal the molecular mechanism of resistance in Kazakh sheep, after selecting the differential miRNA in our previous study, herein, transcriptome analyses were conducted to detect the differential expression genes in the intestinal tissue of Kazakh sheep with resistant and non-resistant MHC haplotypes, after peroral infection with E. granulosus eggs. A total of 3835 differentially expressed genes were identified between the two groups, with 2229 upregulated and 1606 downregulated. Further function analysis showed that the most significant genes were related to both innate immune response and adaptive response participating in the defense against E. granulosus infection and the metabolic changes associated with it. The results suggest that genes related to lectin receptors, NK cells activation, chemokines, and tumor necrosis factor, may play important roles in the response of intestinal tissue to E. granulosus.

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“…Therefore, the roles of Nrf2 in macrophage polarization remain controversial. In addition, it has been reported that 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an AhR-related inducer of the cytochrome P450 1A1 (CYP1A1), enhances the DNA binding activity of nuclear factor-kappaB (NF-κB) and activator protein-1 (AP-1) in macrophages during inflammation or sepsis 50 , 51 . In summary, the precise mechanisms by which AhR regulates macrophage polarization, especially in CaOx nephrocalcinosis, remain ambiguous and require further investigation.…”

Section: Discussionmentioning

confidence: 99%

AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization

Yang¹,

Liu²,

Ye³

et al. 2020

Theranostics

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Calcium oxalate (CaOx) crystal can trigger kidney injury, which contributes to the pathogenesis of nephrocalcinosis. The phenotypes of infiltrating macrophage may impact CaOx-mediated kidney inflammatory injury as well as crystal deposition. How aryl hydrocarbon receptor (AhR) regulates inflammation and macrophage polarization is well understood; however, how it modulates CaOx nephrocalcinosis remains unclear. Methods: Mice were intraperitoneally injected with glyoxylate to establish CaOx nephrocalcinosis model with or without the treatment of AhR activator 6-formylindolo(3,2-b)carbazole (FICZ). Positron emission tomography computed tomography (PET-CT) imaging, Periodic acid-Schiff (PAS) staining, and polarized light optical microscopy were used to evaluate kidney injury and crystal deposition in mice kidney. Western blotting, immunofluorescence, chromatin immunoprecipitation, microRNA-fluorescence in situ hybridization, and luciferase reporter assays were applied to analyze polarization state and regulation mechanism of macrophage. Results: AhR expression was significantly upregulated and negatively correlated with interferon-regulatory factor 1 (IRF1) and hypoxia inducible factor 1-alpha (HIF-1α) levels in a murine CaOx nephrocalcinosis model following administration of FICZ. Moreover, AhR activation suppressed IRF1 and HIF-1α levels and decreased M1 macrophage polarization in vitro . In terms of the mechanism, bioinformatics analysis and chromatin immunoprecipitation assay confirmed that AhR could bind to miR-142a promoter to transcriptionally activate miR-142a. In addition, luciferase reporter assays validated that miR-142a inhibited IRF1 and HIF-1α expression by directly targeting their 3'-untranslated regions. Conclusions: Our results indicated that AhR activation could diminish M1 macrophage polarization and promote M2 macrophage polarization to suppress CaOx nephrocalcinosis via the AhR-miR-142a-IRF1/HIF-1α pathway.

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Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis

Cited by 34 publications

References 56 publications

Particle Size and Biological Fate of ZnO Do Not Cause Acute Toxicity, but Affect Toxicokinetics and Gene Expression Profiles in the Rat Livers after Oral Administration

Particle Size and Biological Fate of ZnO Do Not Cause Acute Toxicity, but Affect Toxicokinetics and Gene Expression Profiles in the Rat Livers after Oral Administration

Intestinal transcriptomes in Kazakh sheep with different haplotypes after experimentalEchinococcus granulosusinfection

AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization

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