Cytochrome P4501A induction and DNA adduct formation in glaucous gulls (Larus hyperboreus), fed with environmentally contaminated gull eggs

Østby, Lene; Gabrielsen, Geir Wing; Krøkje, Åse

doi:10.1016/j.ecoenv.2004.12.023

Cited by 15 publications

(5 citation statements)

References 70 publications

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“…Because glaucous gulls generally are feeding at the highest trophic levels in the arctic marine food web [29], they accumulate some of the highest concentrations of organic contaminant residues of any seabird and mammal species [1]. Levels of OCs in glaucous gulls have been associated with reproductive [30], behavioral [31], developmental [32], genotoxic [33], thyroid [34], and immunological effects [35]. However, these toxicological assessments of glaucous gulls exclusively have dealt with legacy OCs.…”

Section: Introductionmentioning

confidence: 99%

New organochlorine contaminants and metabolites in plasma and eggs of glaucous gulls (Larus hyperboreus) from the Norwegian Arctic

Verreault

Letcher

Muir

et al. 2005

Enviro Toxic and Chemistry

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The present study investigated new or lesser-studied and legacy organochlorine (OC) contaminants and metabolites in plasma and eggs of glaucous gulls (Larus hyperboreus) collected from major breeding colonies on Bear Island in the Norwegian Arctic. Hexachlorobutadiene was below the method limit of detection (< 0.07 ng/g lipid wt) in all samples. The sum (sum) of 20 chlorobornane congener concentrations ranged between 294 and 986 ng/g lipid weight and 104 and 1,121 ng/g lipid weight in plasma and eggs, respectively, whereas those of sum 20polychlorinated naphthalene ranged between 1.34 and 126 ng/g lipid weight in plasma and 1.82 and 162 ng/g lipid weight in eggs. Bis(4-chlorophenyl) sulfone concentrations ranged between 5.24 and 143 ng/ g lipid weight plasma, which is the first report of this contaminant in arctic biota north of Sweden. Based on 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalents (TEQs), partial dioxin-like toxicity varied between 3.04 and 20.8 ng TEQ/g lipid weight in plasma and 0.94 and 46.5 ng TEQ/g lipid weight in eggs, and largely was due to concentrations of non-ortho polychlorinated biphenyls (PCBs), with a very minor contribution from mono-ortho PCBs and polychlorinated naphthalenes. The major aryl sulfone metabolite in plasma was an unidentified hexachlorinated MeSO2-PCB congener (range: 13.5-551 ng/g lipid wt), whereas the pentachlorinated congeners 3'- and 4'-MeSO2-CB101 (range: 4.49-38.1 ng/g lipid wt) dominated in eggs. The predominant halogenated phenolic compound (HPC) in plasma was consistently the PCB metabolite 4-OH-CB187 (range: 0.29-17.5 ng/g wet wt), whereas in eggs, detectable HPCs were at very low and transient concentrations. As part of a complex profile of contaminant exposure, these chemical classes and metabolites may be contributing factors to enhance physiological stress in breeding glaucous gulls.

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Section: Introductionmentioning

confidence: 99%

New organochlorine contaminants and metabolites in plasma and eggs of glaucous gulls (Larus hyperboreus) from the Norwegian Arctic

Verreault

Letcher

Muir

et al. 2005

Enviro Toxic and Chemistry

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“…Because DNA adducts resulted from B a P exposure, these should not have been present. Several 32 P‐postlabeling assay studies have found background levels of DNA adducts in animal and human tissues that were unexposed to other known carcinogens (Watson, ; Østby et al, ). Østby et al () remarked that the presence of background adduct levels in controls is likely due to the presence of contaminants that were transferred from the mother during egg formation or from a common diet.…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al () noted that some studies have correlated CYP1A induction with DNA adducts in fish. However, different studies have also indicated that the correlation between DNA adduct formation and CYP1A is not significant (Østby and KrØkje, ; Østby et al, ).…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 system expression and DNA adduct formation in the liver of Zacco platypus following waterborne Benzo(a)pyrene exposure: implications for biomarker determination

Lee

Kim

Yoon

et al. 2012

Environmental Toxicology

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Benzo(a)pyrene (BaP) is a polycyclic aromatic hydrocarbon that causes mutations and tumor formation. Zacco platypus is a sentinel species that is suitable for monitoring aquatic environments. We studied cytochrome P450 system (CYP system) expression and DNA adduct formation in the liver of Z. platypus following waterborne exposure to BaP. The results showed both dose and time dependency. The significant induction levels of CYP system mRNA and protein reached maximums at 2 days and 14 days, respectively, and hepatosomatic index was maximally induced at 4 days during 14 days BaP exposure. DNA adduct formation was significantly induced compared to corresponding controls (t-test, p < 0.01) after 4 days of exposure in 100 μg/L BaP. These results indicate that the only use of mRNA expression level of CYP system as a biomarker make us underestimate prolonged toxicity (4-14 days) of BaP and the only use of protein expression level of CYP system make us underestimate acute toxicity (1-2 days) of BaP. Therefore, we suggests that a combinational use of the mRNA expression level and protein expression level of CYP system, hepatosomatic index is a useful biomarker in risk assessment of waterborne BaP exposure. In addition, DNA adduct formation was a useful biomarker in risk assessment of waterborne BaP exposure at 4 days. CYP1A was a more sensitive biomarker than CYP reductase for BaP exposure when considering both the mRNA and protein level. Furthermore, our results show that Z. platypus is a useful species for assessing the risk of waterborne BaP exposure.

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“…Not much is known about the genotoxic effects of organochlorine contaminants in Arctic wildlife. Some studies have been carried out, for example, on glaucous gulls (L. hyperboreus) fed environmentally contaminated eggs (Østby et al 2005;Krøkje et al 2006) and on fasting common eiders (Somateria mollissima; Fenstad et al 2014Fenstad et al , 2016. According to the literature, no studies of genotoxicity in wild Arctic fish have been performed.…”

Section: Introductionmentioning

confidence: 99%

DNA Double‐Strand Breaks in Arctic Char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic

Neerland

Bytingsvik

Nikiforov

et al. 2019

Enviro Toxic and Chemistry

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High levels of organochlorine contaminants (OCs) have been found in arctic char (Salvelinus alpinus) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen (n = 18) compared with Laksvatn char (n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char. Environ Toxicol Chem 2019;38:2405–2413. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC

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Cytochrome P4501A induction and DNA adduct formation in glaucous gulls (Larus hyperboreus), fed with environmentally contaminated gull eggs

Cited by 15 publications

References 70 publications

New organochlorine contaminants and metabolites in plasma and eggs of glaucous gulls (Larus hyperboreus) from the Norwegian Arctic

New organochlorine contaminants and metabolites in plasma and eggs of glaucous gulls (Larus hyperboreus) from the Norwegian Arctic

Cytochrome P450 system expression and DNA adduct formation in the liver of Zacco platypus following waterborne Benzo(a)pyrene exposure: implications for biomarker determination

DNA Double‐Strand Breaks in Arctic Char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic

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