Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs

Abstract

A coaxial nanofibrous scaffold of poly (ε-caprolactone) and gelatin/cellulose acetate encapsulating anti-inflammatory and antibacterial drugs was co-electrospun for skin tissue regeneration. Indomethacin and ciprofloxacin as model drugs were added to the core and the shell solutions, respectively. The effect of the drugs’ presence and crosslinking on the scaffold properties was investigated. TEM images confirmed the core–shell structure of the scaffold. The fiber diameter and the pore size of the scaffold increased after crosslinking. The tensile properties of the scaffold improved after crosslinking. The crosslinked scaffold illustrated a higher rate of swelling, and a lower rate of degradation and drug release compared to the uncrosslinked one. Fitting the release data into the Peppas equation showed that Fickian diffusion was the dominant mechanism of drug release from the scaffolds. The results of biocompatibility evaluations showed no cytotoxicity and suitable adhesion and cell growth on the prepared core–shell structure. The antibacterial activity of the scaffolds was studied against one of the most common pathogens in skin wounds, where the existence of ciprofloxacin could prevent the growth of the Staphylococcus aureus bacteria around the scaffold. The obtained results suggested a new coaxial nanofibrous scaffold as a promising candidate for simultaneous tissue regeneration and controlled drug release.

Antiepileptic drug-loaded and multifunctional iron oxide@silica@gelatin nanoparticles for acid-triggered drug delivery

“…Furthermore, the final surface coating of MNPs with a gel shell as validated in Fig. 3 a, is evidenced by the specific absorption bands at 1645 , 1539, 1240, and 3436 cm −1 , which are associated with the functional groups of amide I, II, III, and amide A, respectively 47 .

Figure 3 The physicochemical properties; ( a ) The FTIR spectra of IO MNPs, IO@Si MNPs, and IO@Si@Gel MNPs; ( b ) XRD pattern of IO MNPs, and IO@ Si MNPs; ( c ) DSC thermograms of IO MNPs, IO@Si MNPs, and IO@Si@Gel MNPs; d) VSM curve of IO MNPs and IO@Si@Gel MNPs.

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“…Kinetic analysis of PHT release involved fitting the in vitro release data to several models, which encompassed the zero and first order, Korsmeyer-Peppas, Higuchi, and Parabolic Diffusion models. The aforementioned models were utilized to elucidate both the kinetics and release mechanism of PHT from NPs 47 . The correlation coefficient (R 2 ) of these models was employed to determine the most suitable fitting model.…”

Multifunctional Drug- and AuNRs-Loaded ROS-Responsive Selenium-Containing Polyurethane Nanofibers for Smart Wound Healing

“…Nanofibers can also mimic the natural extracellular matrix (ECM) and enhance the attachment and proliferation of cells required for effective wound healing . The process of producing nanofibers by electrospinning also involves the encapsulation of any kind of hydrophobic, hydrophilic, and biomacromolecular drugs, etc., inside the nanofibers, where they cater the advantage of increased drug storage efficacy and sustained drug release …”

Core–Shell Polyvinyl Alcohol (PVA) Base Electrospinning Microfibers for Drug Delivery

“…In this process, one polymer nanofiber is surrounded by another, resulting in a combination of both materials’ properties, leading to enhanced overall performance [ 21 , 22 , 23 , 24 ]. These systems of encapsulating drugs in polymeric fibers are particularly attractive, with better results than other techniques based on absorption or mixtures, which are frequently used for this purpose [ 25 , 26 , 27 , 28 ]. This technique allows the encapsulation of multiple biomolecules.…”