Cytogenetic Analysis of eight Lily Genotypes

Abstract

-Chromosomal banding markers were established for four lily species -L. candidum, L. × formolongi, L. henryi, L. pumilum and four belonging to horticultural group Oriental hybrids -cultivars -'Alma Ata', 'Expression', 'Marco Polo' and 'Muscadet'. Banding markers were provided by Ag-NOR, CMA 3 /DA/DAPI and C-Giemsa stainings. These techniques enabled to establish markers for identification from 4 to 17 out of 24 chromosomes depending on genotype and technique. Negative DAPI bands were not always observed whereas Ag-NOR and CMA 3 bands were distinctly visible on chromosomes and co-localized the positions of secondary constrictions which enabled to distinguish primary from secondary constrictions, even if these structures were localized close to each other. Additional CMA 3 bands were present intercalary on chromosomes. The C banding method revealed the most markers for chromosomes identification but the technique was difficult to standardisation and results were not fully reproducible. For each genotype tested relative DNA content was evaluated.

Chromosome numbers of 9 taxa of Lilium (Liliaceae) from Turkey

“…The somatic number of Lilium candidum 2n = 24 has been previously reported (Smyth & al. 1989;Agnieszka & al. 2005).…”