Cytogenetical Analyses of some species of the genus Sisymbrium (Brassicaceae) in Turkey

Abstract

Amasya'da yetişen Calepina irregularis türünde ağır metal birikimi ve biyomonitör olarak kullanılabilirliğinin araştırılması Abstract: In this study, heavy metal accumulation (Ni, Fe, Co, Mn) in Calepina irregularis (Asso) Thell. (Brassicaceae), growing naturally in Amasya province, and the usability of it as abiomonitor was investigated. The amount of heavy metals in the root, stem and leaves of plants which were collected from the city center, near the highway, suburban and non-traffic (control) localities, were determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) and the obtained data were evaluated. The Ni, Fe, Co and Mn values in compete plants, growing in traffic areas, were found between the ranges 14.32-35.66 mgkg -1 , 827.61-2716.72 mgkg -1 , 12.52-16.51 mgkg -1 and 175.93-826.75 mgkg -1 respectively. The amount of element accumulation in the plant was listed as Fe>Mn>Ni>Co. Ni and Mn were found to be higher in plants growing near the highway while Fe and Co were higher in plants collected from city centre. Heavy metal accumulation was higher in leaves and roots of the plants growing around the highways while it was higher in stems of the plants growing in suburban areas. According to the correlation with plant and soil samples taken from the localities, the relationship between soil and plant, Fe and Mn contents was found significant at P<0.01 level. This shows that the plant receives Ni and Co elements due to air pollution, and that Fe and Mn are mostly taken from the soil through its roots. According to the results of the study, C.irregularis can be used as a biomonitor since it can monitor the short term changes in environmental pollution in urban areas due to its wide distribution area and it has several individuals in its habitat and its conformity with standard analysis methods.Özet: Bu çalışmada, Amasya ilinde doğal olarak yetişen Calepina irregularis (Asso) Thell. (Brassicaceae) türünde ağır metal birikimi (Ni, Fe, Co, Mn) ve biyomonitör olarak kullanılabilirliği araştırılmıştır. Şehiriçi, otoyol kenarı, kenar semt ve trafiğin olmadığı alanlardan toplanan bitki örneklerinin; kök, gövde ve yapraklarında ağır metal miktarları İndüktif Eşleşmiş Plazma-Optik Emisyon Spektrometresi (ICP-OES) ile belirlenmiş ve elde edilen veriler değerlendirilmiştir. Trafik bulunan alanlarda yetişen bitkilerin toplam kütlesindeki Ni, Fe, Co ve Mn değerleri sırasıyla 14.32-35.66 mgkg -1 , 827.61-2716.72 mgkg -1 , 12.52-16.51 mgkg -1 and 175.93-826.75 mgkg -1 aralığında bulunmuştur. Bitkide element biriktirme miktarı Fe>Mn>Ni>Co şeklinde sıralanmıştır. Ni ve Mn elementi yol kenarında yetişen bireylerde, Fe ve Co ise şehir içinde toplanan örneklerde yüksek değerde tespit edildi. Yol kenarında yetişen bitki örneklerinde yaprak ve kökte ağır metal birikimi daha fazla olurken, kenar semtte yetişen bitkilerde ise gövde de birikim daha fazla bulunmuştur. Lokalitelerden alınan bitki ve toprak örnekleri ile yapılan korelasyona göre toprak ve bitki Fe ve Mn içerikleri arasındaki ilişki P<0.01 düzeyi...

Dianthus cinsi Dentati seksiyonunun (Caryophyllaceae, Sileneae) yeni kromozomal verileri

“…The cytogenetic procedure consisted of the different processes; which were germination in petri dishes at room temperature, pretreatment by α-mono-bromonaphthalene at 4°C for 16 h, fixation by Carnoy's fixative at 4 °C for 24 h, storage in 70% ethanol at 4°C until use, hydrolysis by 1 N HCl at 60°C for 12 min, staining by 2% aceto-orcein for 2h, and preparation by squash method (Eroğlu et al, 2019). At least 10 metaphase microphotographs were used to detect chromosome numbers.…”

Cytotaxonomy of Eight <i>Thlaspi</i> L. <i>Sensu Lato</i> (Brassicaceae) Species Endemic to Turkey

“…(Martin et al 2009), Hesperis L. (Ünal et al 2008), Matthiola L. (Martin et al 2016), Sterigmostemum Bieb. (Sheidai and Sonboli 2011), Aethionema Aiton (Karaismailoğlu 2018), Sisymbrium L. (Eroğlu et al 2019), Arabis L. genera (Şirin 2020) and Cardamine L. (Karaismailoğlu 2021) in the family have been examined in Turkey. The absence of cytotaxonomical examinations on species of the genus Thlaspi s.l.…”