Cytokeratin expression in squamous metaplasia of the human uterine cervix

Stable Expression of Angiopoietin-1 and Other Markers by Cultured Pericytes: Phenotypic Similarities to a Subpopulation of Cells in Maturing Vessels During Later Stages of Angiogenesis In Vivo

“…As expected, antibodies to CD31 stained endothelial cells in both large and small vessels, whereas PAL-E staining was largely confined to microvascular (Parums et al, 1990) ϩϩϩ ϩϩϩ 0 0 PAL-E, Sanbios, Netherlands (Schlingemann et al, 1985) Ϯ ϩϩϩ 0 0 Pericytes/smooth muscle cells Desmin, DAKO, ϩϩϩ ϩϩϩ 0 0 mAb clone D33 (Van Muijen et al, 1987) Smooth muscle ␣-actin, Sigma, ϩϩϩ ϩϩϩ 0 0 mAb clone 1A4 (Skalli et al, 1986) ␣1␤1 integrin, T cell diagnostics, Woburn, Massachusetts, ϩ ϩϩϩ 0 0 mAb clone TS2/7 (Hemler, 1990) ␣ and ␥-actin, DAKO, ϩ ϩϩϩ 0 0 mAb clone HHF35 (Gown et al, 1986) Calponin, Sigma, ϩϩϩ Ϯ 0 0 mAb clone hCP Smooth muscle myosin, Sigma, ϩϩϩ ϩ 0 0 mAb clone hSM-V (Longtine et al, 1985) HMW-MAA, Sanbios, Netherlands ϩ 0 0 0 mAb clone NKI-M6 (Natali et al, 1981) PDGF ␤-receptor, 0 0 0 0 mAb Clone PDGFR-␤ 2 (Heldin et al, 1988) Fibroblasts Procollagen type 1 c-propeptide, 0 0 0 0 Biogenesis, Sandown, New Hampshire (MacDonald et al, 1986) Prolyl-4-hydroxylase, DAKO 0 0 0 0 mAb clone 5B5 (Höyhtyä et al, 1984) Pericytes and fibroblasts ASO2, Dianova, Hamburg, Germany Ϯ b ϩϩ ϩϩϩ 0 mAb clone ASO2 (Saalbach et al, 1996) Epithelium Cytokeratin, Sigma (Gigi-Leitner et al, 1986) 0 0 0 ϩϩϩ a 0, no positive cells; Ϯ, Ͻ10% of cells positive; ϩ, 10% to 40% of cells positive; ϩϩ, 40% to 70% of cells positive; ϩϩϩ, 70% to 90% of cells positive. b A few cells in the smooth muscle layer stained intensely for ASO2, whereas most smooth muscle cells were negative.…”

Immunohistochemical Study of Cytokeratin Expression in Subcolumnar Reserve Cells of Human Uterine Cervix .

“…18 antibodies recognize different epitopes, or exhibit varying affinities for their respective antigens. As we and others have shown, these cytokeratins are expressed in various simple and glandular epithelia, including endocervical columnar cells (6,13). This shows a relationship between the endocervical columnar and reserve cells.…”

Keratin Expression in Normal Cervical Epithelium, Cervical Intraepithelial Neoplasia and Cervical Cancer.

“…Thus, keratins have proved to be efficient tools for characterizing the differentiation of a particular cell type and for establishing the origin of certain cells. The keratin expression patterns in the normal cervix are known to a large extent, using specific monoclonal antibodies against the individual keratin polypeptides [5,6,8,9,16]. The distribution of the keratins in the normal cervix can be summarized as follows: ectocervical nonkeratinizing squamous epithelium shows a keratin pattern characterized by intense expression of keratins 4 and 13.…”