Cytokine‐Induced Expansion of Human CD34+Stem/Progenitor and CD34+CD41+Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

Ahmed, Nazish; Khokher, M. A.; Hassan, H. T.

doi:10.1002/stem.170092

Cited by 28 publications

(20 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22 As we report here, irradiation induces a profound perturbation of the BM microenvironment, composed of vascular damage and a shift in the location of megakaryocytes to the endosteal surface, where they maintain close contact with osteoblasts, suggesting interactions between these 2 cell types. Indeed, there are compelling in vitro data to support the concept that osteoblasts can maintain the growth and differentiation of megakaryocytes 47,48 and that contact between megakaryocytes and osteoblast precursors facilitates proliferation and differentiation of the latter. 49 Although the intent of cytotoxic conditioning regimen is to eradicate host BM cellularity, we noted that many resident megakaryocytes, osteoblasts, and reticular bone lining cells survived and even proliferated up to 48 hours after TBI.…”

Section: Discussionmentioning

confidence: 99%

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Dominici

Rasini

Bussolari

et al. 2009

Blood

185

172

View full text Add to dashboard Cite

Adequate recovery of hematopoietic stem cell (HSC) niches after cytotoxic conditioning regimens is essential to successful bone marrow transplantation. Yet, very little is known about the mechanisms that drive the restoration of these niches after bone marrow injury. Here we describe a profound disruption of the marrow microenvironment after lethal total body irradiation of mice that leads to the generation of osteoblasts restoring the HSC niche,

show abstract

Section: Discussionmentioning

confidence: 99%

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Dominici

Rasini

Bussolari

et al. 2009

Blood

185

172

View full text Add to dashboard Cite

show abstract

“…IL-3 alone or in combination with other early-acting hematopoietic growth factors, such as stem cell factor (SCF), IL-1, and IL-6, has been shown to support the multilineage colony formation and expansion to different lineages in liquid culture [1][2][3][4][5][6][7][8]. In addition, IL-3 has been used in culture to produce blood cells of various lineages, including neutrophils, monocytes, erythrocytes, megakaryocytes, basophils, eosinophils, mast cells, and B-lymphoid cells [2,5,[9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Correlation Between IL‐3 Receptor Expression and Growth Potential of Human CD34 ⁺ Hematopoietic Cells from Different Tissues

Huang¹,

Chen²,

Yu³

et al. 1999

STEM CELLS

View full text Add to dashboard Cite

CD123 (␣-subunit of IL-3 receptor) expression on primitive and committed human hematopoietic cells was studied by multicolor sorting and single-cell culture. The sources of cells included fetal liver (FLV), fetal bone marrow, umbilical cord blood, adult bone marrow and mobilized peripheral blood. Three subsets of CD34 + cells were defined by the levels of surface CD123: CD123 negative , CD123 low , and CD123 bright . Coexpression of lineage markers showed that a majority of CD34 + CD123 bright cells were myeloid and B-lymphoid progenitors, while erythroid progenitors were mainly in the CD34 + CD123 negative subset. The CD34 + CD123 low subset contained a heterogeneous distribution of early and committed progenitor cells. Single CD34 + cells from the CD123 subsets were cultured in a cytokine cocktail of stem cell factor, interleukin 3 (IL-3), IL-6, GM-CSF, erythropoietin, insulin-like growth factor-1, and basic fibroblast growth factor. After 14 days of incubation, a higher cloning efficiency (CE) was observed in the CD34 + CD123 negative and CD34 + CD123 low fractions (37 ± 23% and 44 ± 23%, respectively) than in the CD34 + CD123 bright fraction (15 ± 21%). Using previously published criteria that colonies containing dispersed, translucent cells (dispersed growth pattern, DGP) were derived from primitive cells and that colonies composed solely of clusters were from committed cells, early precursors were distributed evenly in the CD34 + CD123 negative and CD34 + CD123 low subsets. When CD38 and CD90 (Thy-1) were used for further characterization of CD34 + cells from FLV, CE increased from 37 ± 23% in CD123 negative to 70 ± 19% in CD123 negative CD38 -and from 44 ± 23% in CD123 low to 66 ± 19% in CD123 low CD38 -. No significant increase in CE or DGP progenitors was observed when CD34 + cells were sorted by CD90 and CD123. We concluded that: A) high levels of CD123 were expressed on B-lymphoid and myeloid progenitors; B) early erythroid progenitors had little or no surface CD123, and C) primitive hematopoietic cells are characterized by CD123 negative/low expression.

show abstract

“…Human osteoblasts have been reported to produce several hematopoietic cytokines, including IL-1β, IL-6, IL-7, G-CSF, M-CSF, GM-CSF, tumor necrosis factor-α, LIF, OPG, receptor activator of NF-ĸB ligand, SDF-1, VEGF, and osteoclast differentiation factor [1, 2,10,11,12], and not to produce IL-1α, IL-3, or SCF [10]. However, in our experiment, human osteoblasts did not produce IL-7, G-CSF, M-CSF, or GM-CSF.…”

Section: Discussionmentioning

confidence: 99%

Human Osteoblasts Support Hematopoietic Cell Development in vitro

et al. 2008

View full text Add to dashboard Cite

Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.

show abstract

Cytokine‐Induced Expansion of Human CD34⁺Stem/Progenitor and CD34⁺CD41⁺Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

Abstract: ABSTRACT

Cited by 28 publications

References 44 publications

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Correlation Between IL‐3 Receptor Expression and Growth Potential of Human CD34 ⁺ Hematopoietic Cells from Different Tissues

Human Osteoblasts Support Hematopoietic Cell Development in vitro

Contact Info

Product

Resources

About

Cytokine‐Induced Expansion of Human CD34+Stem/Progenitor and CD34+CD41+Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

Abstract: ABSTRACT

Cited by 28 publications

References 44 publications

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation

Correlation Between IL‐3 Receptor Expression and Growth Potential of Human CD34 + Hematopoietic Cells from Different Tissues

Human Osteoblasts Support Hematopoietic Cell Development in vitro

Contact Info

Product

Resources

About

Cytokine‐Induced Expansion of Human CD34⁺Stem/Progenitor and CD34⁺CD41⁺Early Megakaryocytic Marrow Cells Cultured on Normal Osteoblasts

Correlation Between IL‐3 Receptor Expression and Growth Potential of Human CD34 ⁺ Hematopoietic Cells from Different Tissues