Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.)

Vasudevan, Rajeswari; Staden, J. Van

doi:10.1007/s11240-011-9964-0

Cited by 40 publications

(19 citation statements)

References 27 publications

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“…Moreover, in vitro regeneration rates of explants of the orchid Ansellia africana Lindl. are significantly higher in m TR-containing media than in other tested media [ 99 ], and it has proposed utility as an alternative to other commonly used CKs in micropropagation of smoke bush ( Cotinus coggygria Scop.) [ 100 ].…”

Section: N7- and N9-sugar Cytokinin Conjugatesmentioning

confidence: 99%

Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back

et al. 2020

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Cytokinins and their sugar or non-sugar conjugates are very active growth-promoting factors in plants, although they occur at very low concentrations. These compounds have been identified in numerous plant species. This review predominantly focuses on 9-substituted adenine-based cytokinin conjugates, both artificial and endogenous, sugar and non-sugar, and their roles in plants. Acquired information about their biological activities, interconversions, and metabolism improves understanding of their mechanisms of action and functions in planta. Although a number of 9-substituted cytokinins occur endogenously, many have also been prepared in laboratories to facilitate the clarification of their physiological roles and the determination of their biological properties. Here, we chart advances in knowledge of 9-substituted cytokinin conjugates from their discovery to current understanding and reciprocal interactions between biological properties and associated structural motifs. Current organic chemistry enables preparation of derivatives with better biological properties, such as improved anti-senescence, strong cell division stimulation, shoot forming, or more persistent stress tolerance compared to endogenous or canonical cytokinins. Many artificial cytokinin conjugates stimulate higher mass production than naturally occurring cytokinins, improve rooting, or simply have high stability or bioavailability. Thus, knowledge of the biosynthesis, metabolism, and activity of 9-substituted cytokinins in various plant species extends the scope for exploiting both natural and artificially prepared cytokinins in plant biotechnology, tissue culture, and agriculture.

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Section: N7- and N9-sugar Cytokinin Conjugatesmentioning

confidence: 99%

Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back

et al. 2020

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“…BAP, Kin and 2iP were less effective in comparison with TDZ in terms of the shoot number per node and the response percentages (Table 1). The high impact of TDZ on axillary shoot proliferation has been reported in number of plant species (Faisal et al 2005, Noshad et al 2009, Vasudevan & van Staden 2011.…”

Section: Resultsmentioning

confidence: 94%

Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Kher

Joshi

Nekkala

et al. 2014

Journal of Horticultural Research

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Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

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“…The superiority of mT and its derivatives in improving shoot proliferation over the conventional cytokinins like BAP and Kn was demonstrated by some researchers in recent past (Amoo and Van Staden 2013;Aremu et al 2012;Bairu et al 2007;Baroja-Fernández et al 2002;Bose et al 2016;Gentile et al 2014;Roels et al 2005). However, a few reports on the use of mT in orchid tissue culture indicate that this group of topolins could be a new source of aromatic cytokinins with high multiplication efficiency and improved physiological activity in micropropagated plants (Vasudevan and Van Staden 2011;Bhattacharyya et al 2016). It was possible to produce an average of 22.5 buds or shoots from each tTCL explant within 40-60 days.…”

Section: Results and Discussion Ttcl Mediated Organogenesismentioning

confidence: 99%

Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis and ex vitro acclimatization of Malaxis wallichii, a threatened medicinal orchid

Bose

Kumaria

Choudhury

et al. 2017

Physiol Mol Biol Plants

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(Lindl.) Deb, a small, perennial, monopodial, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000 m asl. The pseudobulbs are important ingredients of century old drug 'Ashtavarga' and a polyherbal energetic tonic 'Chyavanprash'. An efficient genetically stable in vitro propagation protocol using transverse thin cell layer culture system was established for . In the present report, meta-topolin alone proved to be three times more beneficial compared to other routinely used cytokinins in inducing highest number of shoot buds, plant height and growth of regenerated shoots. The highest regeneration frequency (89%) along with maximum number of adventitious shoots per explant (22.5 ± 0.6) was observed in MS medium supplemented with 1.0 mg/l meta-topolin and 0.5 mg/l α-naphthalene acetic acid. Highest rooting frequency with highest number of roots (8.66 ± 0.3) was achieved in half-strength MS medium fortified with 1.0 mg/l indole acetic acid. Clonal stability of in vitro-derived plantlets was evaluated and compared to donor plant using intron splice junction (ISJ) markers and flow cytometry. ISJ markers revealed 4.76% clonal variability indicating high degree of genetic stability amongst the in vitro-derived regenerants. The nuclear DNA content of (2n) was found to be 2C = 2.760 ± 0.02 pg and therefore, 1349.64 Mbp (1C). Flow cytometry analysis of actively growing young and mature leaves from donor as well as in vitro-derived plantlets revealed presence of three peaks corresponding to 2C, 4C and 8C, while 2C was the most abundant. In the present investigation, there was no significant difference in the 2C DNA content between the mother and in vitro-derived plants; however, the frequency of endopolyploid cells varied in young and adult plants. An increased HO content as well as lipid peroxidation activities were observed during early stages of acclimatization which declined afterwards. The enhanced activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase in acclimatized plantlets as compared to in vitro-grown ones revealed their active involvement in growth and development against oxidative stress under external adverse environment.

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Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.)

Cited by 40 publications

References 27 publications

Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back

Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back

Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis and ex vitro acclimatization of Malaxis wallichii, a threatened medicinal orchid

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