Micropropagation of <i>PLUCHEA LANCEOLATA</i> (Oliver &amp; Hiern.) Using Nodal Explant

Kher, Mafatlal M.; Joshi, Dimpal; Nekkala, Sureshkumar; Nataraj, M.; Raykundaliya, Dharmesh P.

doi:10.2478/johr-2014-0004

Cited by 21 publications

(8 citation statements)

References 20 publications

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“…1B) after that the shoot length was reduced significantly along with vitrification. The increase in number of shoots may be due to suppression of apical dominance during subculture [26], [48]. After 4–5th subculture maximum 16.45 ± 0.44 shoots could be obtained from a single node in this study on MS medium augmented with 0.5 mg l −1 of each of BAP and Kin (Table 2).…”

Section: Resultsmentioning

confidence: 69%

In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures

Shekhawat¹,

Kannan²,

Manokari³

et al. 2015

Journal of Genetic Engineering and Biotechnology

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Methods were developed in the present investigation for cloning and large scale plant production of Passiflora foetida L. germplasm selected from the East-Coast region of South India. Nodal shoot segments were used as explants. The explants were dressed and surface sterilized with 0.1% (w/v) HgCl 2 . Multiple shoots were induced (6.13 ± 0.22 shoots per explant) by proliferation of nodal shoot meristems on Murashige and Skoog (MS) semi-solid medium + 2.0 mg l À1 6-benzylaminopurine (BAP). The shoots of P. foetida were further multiplied (16.45 ± 0.44 shoots per explant) on MS medium + 0.5 mg l À1 each of BAP and Kinetin (Kin). The in vitro generated shoots were rooted on half-strength MS medium containing 2.5 mg l À1 indole-3 butyric acid (IBA). By this method 67% shoots were rooted. About 97% shoots were rooted ex vitro (8.33 ± 0.29 roots per shoot) when the cut ends of the shoots were treated with 300 mg l À1 IBA for 5 min. The in vitro rooted plants were hardened and acclimatized in the greenhouse and successfully (100%) transplanted to the field. Ó 2015 Production and hosting by Elsevier B.V. on behalf

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Section: Resultsmentioning

confidence: 69%

In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures

Shekhawat¹,

Kannan²,

Manokari³

et al. 2015

Journal of Genetic Engineering and Biotechnology

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“…1 b–g). The reports from Lindiro et al ( 2013 ) in Chrysanthemum cinerariaefolium and Kher et al ( 2014 ) in Pluchea lanceolata supported this result. In divergence to the reports on TDZ and 2-iP, Kn in Matthiola incana (Hesar et al 2011 ) and BAP with NAA in Vigna subterranea (Kone et al 2013 ) have also proved better for the multiplication of shoots.…”

Section: Resultsmentioning

confidence: 70%

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Mittal

Sharma

2017

3 Biotech

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Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of multiple shoot formation was induced when the nodal segments were cultured on MS medium supplemented with BAP (1.0 mg L−1) and 2-iP (0.5 mg L−1). The highest mean number of shoots per nodal explant (7.9 ± 0.45) with highest shoot length (9.3 ± 0.48 cm) and 86% response were achieved on this media and hormonal concentration. The optimum rooting was obtained on ½ strength of MS medium augmented with IBA (0.5 mg L−1) with 8.3 ± 0.46 cm root length and 89% response. Micropropagated plantlets were found to be identical with the mother plant when clonal fidelity of these plantlets were analyzed with inter simple sequence repeat (ISSR) marker. The berberine content was analyzed through LCMS QToF and the highest amount was found in in vitro callus (19.8 µg/gm) followed by stem (9.3 µg/gm) and leaves of field-grown plants (8.4 µg/gm). Further, presence of berberine was confirmed by ESI–MS spectra with protonated molecular ions ([M + H]+) at m/z 336. Furthermore, MS–MS fragmentation pattern confirmed for the presence of berberine in both the samples. Both the spectra (standard and samples) showed common peaks for berberine in the form of protonated molecular ions ([M + H]+) at m/z 320, m/z 304, m/z 292, m/z 278 in MS/MS mode. The study revealed that developed protocol is potent for rapid mass propagation of this plant species with high accumulation of important secondary metabolite berberine.

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“…Plant tissue culture techniques are used for in situ conservation of many medicinally important plants (Bharvad & Mohan 2012;Inamdar et al 1990;Kher et al 2014;Mehta & Subramanian 2005;Sharma & Mohan 2006a, b;Sharma et al 2007;Varghese et al 1993aVarghese et al , b, 1992. Axillary shoot proliferation technique is popularly used for clonal propagation in order to produce true to type and a high quality plants.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

Kher

Nataraj

Parmar

et al. 2015

Journal of Horticultural Research

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Merremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm -3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm -3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm -3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

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Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

Cited by 21 publications

References 20 publications

In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures

In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

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