Cytological events in tomato leaves inoculated with conidia of Blumeria graminis f. sp. hordei and Oidium neolycopersici KTP-01

Sameshima, Takeshi; Kashimoto, Koichi; Kida, Keiko; Matsuda, Yoshinori; Nonomura, Teruo; Kakutani, Koji; Nakata, Kengo; Kusakari, Shin-ichi; Toyoda, Hideyoshi

doi:10.1007/s10327-003-0078-3

Cited by 21 publications

(9 citation statements)

References 15 publications

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“…This hypothesis was supported by the observation of the conidia inoculated into nonhost barley leaves. Barley leaves suppress the penetration of nonpathogenic powdery mildew pathogens into epidermal cells by the formation of papillae (Mayama & Shishiyama 1978;Kita et al 1981;Tosa & Shishiyama 1984;Sameshima et al 2004). In fact, the tomato powdery mildew conidia were prevented from penetrating into barley leaves by the papillae formed at the sites of all appressorial projections (Fig 1A, B).…”

Section: Discussionmentioning

confidence: 92%

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

et al. 2010

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Section: Discussionmentioning

confidence: 92%

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

et al. 2010

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“…In addition, samples were prepared for a BX-60 light microscope (BX-60 LM; Olympus, Tokyo, Japan). For this, samples were fixed and chlorophyll was removed in a boiling alcoholic lactophenol solution (10 mL glycerol, 10 mL phenol, 10 mL lactic acid, 10 mL distilled water and 40 mL 99.8% ethanol) for 1–2 min and then stained with 0.1% Aniline Blue (Nacalai Tesque, Tokyo, Japan) dissolved in distilled water as described previously [ 71 ]. The samples were observed under a BX-60 LM.…”

Section: Methodsmentioning

confidence: 99%

Ampelomyces strains isolated from diverse powdery mildew hosts in Japan: Their phylogeny and mycoparasitic activity, including timing and quantifying mycoparasitism of Pseudoidium neolycopersici on tomato

et al. 2021

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A total of 26 Ampelomyces strains were isolated from mycelia of six different powdery mildew species that naturally infected their host plants in Japan. These were characterized based on morphological characteristics and sequences of ribosomal DNA internal transcribed spacer (rDNA-ITS) regions and actin gene (ACT) fragments. Collected strains represented six different genotypes and were accommodated in three different clades of the genus Ampelomyces. Morphology of the strains agreed with that of other Ampelomyces strains, but none of the examined characters were associated with any groups identified in the genetic analysis. Five powdery mildew species were inoculated with eight selected Ampelomyces strains to study their mycoparasitic activity. In the inoculation experiments, all Ampelomyces strains successfully infected all tested powdery mildew species, and showed no significant differences in their mycoparasitic activity as determined by the number of Ampelomyces pycnidia developed in powdery mildew colonies. The mycoparasitic interaction between the eight selected Ampelomyces strains and the tomato powdery mildew fungus (Pseudoidium neolycopersici strain KTP-03) was studied experimentally in the laboratory using digital microscopic technologies. It was documented that the spores of the mycoparasites germinated on tomato leaves and their hyphae penetrated the hyphae of Ps. neolycopersici. Ampelomyces hyphae continued their growth internally, which initiated the atrophy of the powdery mildew conidiophores 5 days post inoculation (dpi); caused atrophy 6 dpi; and complete collapse of the parasitized conidiphores 7 dpi. Ampelomyces strains produced new intracellular pycnidia in Ps. neolycopersici conidiophores ca. 8–10 dpi, when Ps. neolycopersici hyphae were successfully destroyed by the mycoparasitic strain. Mature pycnidia released spores ca. 10–14 dpi, which became the sources of subsequent infections of the intact powdery mildew hyphae. Mature pycnidia contained each ca. 200 to 1,500 spores depending on the mycohost species and Ampelomyces strain. This is the first detailed analysis of Ampelomyces strains isolated in Japan, and the first timing and quantification of mycoparasitism of Ps. neolycopersici on tomato by phylogenetically diverse Ampelomyces strains using digital microscopic technologies. The developed model system is useful for future biocontrol and ecological studies on Ampelomyces mycoparasites.

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“…In the nonhost interaction of tomato‐barley powdery mildew fungus ( B. graminis f. sp. hordei , Bgh ), it is the hypersensitive cell death rather than papillae that plays a pivotal role in arrest of fungal development, and the generation of HR is also delayed (Sameshima et al, ). Papillae, H 2 O 2 and HR play important roles in preventing the development of Bgt on nonhost pepper, although the time when H 2 O 2 production reaches the peak is much earlier in the nonhost interaction than in the host interaction (Hao et al, ).…”

Section: Introductionmentioning

confidence: 99%

Histochemical comparison of the nonhost tomato with resistant wheat against Blumeria graminis f. sp. tritici

Hao

Chen

Zhang

et al. 2013

Microscopy Res & Technique

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The nonhost interaction of tomato-Blumeria graminis f. sp. tritici (Bgt) and resistant host interaction of wheat-Bgt were compared histochemically. The percentage of appressorium formation had no significant difference on tomato and wheat leaves. Papilla formation occurred earlier and more on host wheat than on nonhost tomato leaves, while the incidence of hypersensitive cell death was much higher in the nonhost interaction. Whole-cell H2O2 accumulation and hypersensitive cell death usually appeared in haustorium-invaded wheat epidermal cells. In contrast, the vast majority of non-haustorium epidermal cells were associated with H2O2 accumulation and hypersensitive cell death on tomato. Localized H2O2 accumulation and hypersensitive response were detected in effective papillae in both interactions. The peak percentage of haustorium formation was less than 7% in the nonhost interaction while reached 43% in the incompatible host interaction. These results indicate that hypersensitive cell death and papillae are likely to play an important role in preventing Bgt penetration and development on tomato and wheat leaves, both defense responses involving H2O2 accumulation. This study further implies that the nonhost and incompatible interactions share similar cytological mechanisms.

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Cytological events in tomato leaves inoculated with conidia of Blumeria graminis f. sp. hordei and Oidium neolycopersici KTP-01

Cited by 21 publications

References 15 publications

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

Ampelomyces strains isolated from diverse powdery mildew hosts in Japan: Their phylogeny and mycoparasitic activity, including timing and quantifying mycoparasitism of Pseudoidium neolycopersici on tomato

Histochemical comparison of the nonhost tomato with resistant wheat against Blumeria graminis f. sp. tritici

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