Cytolytic peptides induce biphasic permeability changes in mammalian cell membranes

Su, Mei; He, Chunbo; West, Charles A.; Mentzer, Steven J.

doi:10.1016/s0022-1759(01)00334-9

Cited by 21 publications

(16 citation statements)

References 29 publications

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“…This biphasic kinetics of melittin-induced permeabilization has been supported by experiments carried out using model membranes (Ghosh et al 1997;El Jastimi and Lafleur, 1999;Gó mara et al 2003;Allende et al 2005) as well as in nucleated mammalian cells (Su et al 2001). Interestingly, the rates of the fast and slow kinetic phases are concentration-dependent and these rates have been interpreted in terms of the molecularity of the melittin species involved in each phase (DeGrado et al 1982).…”

Section: Hemolytic Activitymentioning

confidence: 70%

Melittin: a Membrane-active Peptide with Diverse Functions

2007

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Melittin is the principal toxic component in the venom of the European honey bee Apis mellifera and is a cationic, hemolytic peptide. It is a small linear peptide composed of 26 amino acid residues in which the amino-terminal region is predominantly hydrophobic whereas the carboxy-terminal region is hydrophilic due to the presence of a stretch of positively charged amino acids. This amphiphilic property of melittin has resulted in melittin being used as a suitable model peptide for monitoring lipid-protein interactions in membranes. In this review, the solution and membrane properties of melittin are highlighted, with an emphasis on melittin-membrane interaction using biophysical approaches. The recent applications of melittin in various cellular processes are discussed.

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Section: Hemolytic Activitymentioning

confidence: 70%

Melittin: a Membrane-active Peptide with Diverse Functions

2007

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“…Further, no direct data are available attesting to Hla-induced membrane damage in the intact alveolar epithelium, where membrane damage might be repaired spontaneously (19,33). To test this hypothesis, we evaluated epithelial membrane damage at the MA-alveolar interface in terms of epithelial calcein fluorescence, which decreases following membrane damage (34). After alveolar instillation of USA300 WT , calcein fluorescence decreased in MA-associated epithelium ( Figure 3, A and B, first bar from left), signifying epithelial membrane damage.…”

Section: Resultsmentioning

confidence: 99%

“…To test this possibility, we determined survival in USA300-infected mice. Infection with WT USA300 caused rapid, severe mortality, suggesting that severe pulmonary edema induced hypoxia and, posfying damage to the epithelial membrane (34). Clustered bacteria that failed to form bacterial adhesive interactions, namely the PhnD-deficient mutant, did not induce epithelial injury.…”

Section: Discussionmentioning

confidence: 98%

Disruption of staphylococcal aggregation protects against lethal lung injury

Hook

Islam

Parker

et al. 2018

Journal of Clinical Investigation

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“…The data were analyzed by FCS Express 4 (De Novo Software, Los Angeles, CA) and described cell marker profiling as dual parameter panels. Debris and dead cells were excluded based on forward and side scatter characteristics and 7AAD staining (Su et al, 2001). …”

Section: Methodsmentioning

confidence: 99%

Cross‐circulation and cell distribution kinetics in parabiotic mice

Gibney

Chamoto

Lee

et al. 2011

Journal Cellular Physiology

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Blood-borne nucleated cells participate not only in inflammation, but in tissue repair and regeneration. Because progenitor and stem cell populations have a low concentration in the blood, the circulation kinetics and tissue distribution of these cells is largely unknown. An important approach to tracking cell lineage is the use of fluorescent tracers and parabiotic models of cross-circulation. Here, we investigated the cross-circulation and cell distribution kinetics of C57/B6 GFP+/wild-type parabionts. Flow cytometry analysis of the peripheral blood after parabiosis demonstrated no evidence for a “parabiotic barrier” based on cell size or surface characterstics; all peripheral blood cell subpopulations in this study reached equilibrium within 14 days. Whole blood fluorescence analysis indicated that the mean exchange flow rate was 16μl/hr or 0.66% of the circulating blood volume per hour. Studies of peripheral lymphoid organs indicated differential cell distribution kinetics. Some subpopulations, such as CD8+ and CD11c+, equilibrated in both lymph nodes and spleen indicating a residence time less than 28 days; in contrast, other lymphocyte subpopulations, such as B220+ and CD4+ cells, had not yet reached equilibrium at 28 days. We conclude that parabiosis can provide important insights into defining tissue distribution, residence times, and recirculating pools using fluorochrome markers of cell lineage.

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Cytolytic peptides induce biphasic permeability changes in mammalian cell membranes

Cited by 21 publications

References 29 publications

Melittin: a Membrane-active Peptide with Diverse Functions

Melittin: a Membrane-active Peptide with Diverse Functions

Disruption of staphylococcal aggregation protects against lethal lung injury

Cross‐circulation and cell distribution kinetics in parabiotic mice

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